The Entamoeba histolytica Ehcp112 gene has a distal and weak promoter

Ehcp112 encodes the Entamoeba histolytica EhCP112 cysteine protease that is part of the EhCPADH complex. By in silico analysis we identified putative transcription factor-binding sites along 837 bp upstream the Ehcp112 gene ATG codon. A TATA-like motif (TATATAAA) was located at −36 to −29 bp, a GAAC box (GAACC) was found at −10 to −14 bp and an Inr sequence (TTCAAC) at −8 to −2 bp. These tripartite promoter elements are in non-canonical...