Targeting endogenous nuclear antigens by electrotransfer of monoclonal antibodies in living cells
Abstract
Antibodies are valuable tools for functional studies in vitro, but their use in living cells remains challenging because they do not naturally cross the cell membrane. Here, we present a simple and highly efficient method for the intracytoplasmic delivery of any antibody into cultured cells. By following the fate of monoclonal antibodies that bind to nuclear antigens, it was possible to image endogenous targets and to show that inhibitory...
Paper Details
Title
Targeting endogenous nuclear antigens by electrotransfer of monoclonal antibodies in living cells
Published Date
Jul 1, 2013
Journal
Volume
5
Issue
4
Pages
518 - 522
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Notes
History