Human unrestricted somatic stem cells loaded in nanofibrous PCL scaffold and their healing effect on skin defects

Published on Aug 17, 2016in Artificial Cells Nanomedicine and Biotechnology3.343
· DOI :10.3109/21691401.2015.1062390
H Bahrami3
Estimated H-index: 3
(Tehran University of Medical Sciences),
Saeed Heidari Keshel18
Estimated H-index: 18
(Shahid Beheshti University of Medical Sciences and Health Services)
+ 1 AuthorsEsmaeil Biazar24
Estimated H-index: 24
(IAU: Islamic Azad University)
Sources
Abstract
Unrestricted somatic stem cells (USSCs) loaded in nanofibrous polycaprolactone (PCL) scaffolds can be used for skin regeneration when grafted onto full-thickness skin defects of rats. Nanofibrous PCL scaffolds were designed by the electrospinning method and crosslinked with laminin protein. Afterwards, the scaffolds were evaluated by scanning electron microscopy, and physical and mechanical assays. In this study, nanofibrous PCL scaffolds loaded with USSCs were grafted onto the skin defects. The wounds were subsequently investigated 21 days after grafting. Results of mechanical and physical analyses showed good resilience and compliance to movement as a skin graft. In animal models; study samples exhibited the most pronounced effect on wound closure, with statistically significant improvement in wound healing being seen at 21 days post-operatively. Histological examinations of healed wounds from all samples showed a thin epidermis plus recovered skin appendages in the dermal layer for samples with cell. T...
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