Unrestricted somatic stem cells (USSCs) loaded in nanofibrous polycaprolactone (PCL) scaffolds can be used for skin regeneration when grafted onto full-thickness skin defects of rats. Nanofibrous PCL scaffolds were designed by the electrospinning method and crosslinked with laminin protein. Afterwards, the scaffolds were evaluated by scanning electron microscopy, and physical and mechanical assays. In this study, nanofibrous PCL scaffolds loaded with USSCs were grafted onto the skin defects. The wounds were subsequently investigated 21 days after grafting. Results of mechanical and physical analyses showed good resilience and compliance to movement as a skin graft. In animal models; study samples exhibited the most pronounced effect on wound closure, with statistically significant improvement in wound healing being seen at 21 days post-operatively. Histological examinations of healed wounds from all samples showed a thin epidermis plus recovered skin appendages in the dermal layer for samples with cell. T...
Nanofibers have improved the performance of biomaterials, and can be considered effective. In this study, PHBV nanofibers were designed and then modified and crosslinked by oxygen plasma and laminin. The samples were evaluated by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscope (SEM), contact angle, mechanical analyses, and cell culture. ATR-FTIR structural analysis showed the presence of functional groups on the nanofibrous surfaces. ...
#1Esmaeil Biazar(IAU: Islamic Azad University)H-Index: 24
#2Saeed Heidari Keshel(Shahid Beheshti University of Medical Sciences and Health Services)H-Index: 18
AbstractThe aim of this study was to produce a chitosan-crosslinked nanofibrous biodegradable poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nerve conduit. The artificial scaffold was designed by electrospinning method, and cross-linked with chitosan by chemical method. The scaffolds were evaluated by microscopic, physical, and mechanical analyses, and cell culture assays with Schwann cells. Results of analyses showed a good resilience and compliance with movement as a neural graft. Cellul...
#1Esmaeil Biazar(IAU: Islamic Azad University)H-Index: 24
Polyhydroxyalkanoates (PHAs) have been demonstrated to be a family of biopolymers with good biodegradability and biocompatibility. Designed scaffolds comprising natural and synthetic materials are now widely used in the reconstruction of damaged tissues especially neural tissues. Polyhydroxyalkanoate scaffolds with properties similar to neural structures can be more effective in the reconstruction process. Better cell adhesion and migration, more guiding of axons, and structural features, such a...
Unrestricted somatic stem cells (USSCs) loaded in nanofi brous PHBV scaff old can be used for skin regeneration when grafted into full-thickness skin defects of rats. Nanofi brous PHBV scaff olds were designed using electrospinning method and then, modifi ed with the immobilized collagen via the plasma method. After ward, the scaff olds were evaluated using scanning electron microscopy, physical and mechanical assays. In this study; nanofi brous PHBV scaff olds loaded with and without USSCs were...
#1Esmaeil Biazar(IAU: Islamic Azad University)H-Index: 24
#2S. Heidari Keshel(Shahid Beheshti University of Medical Sciences and Health Services)H-Index: 2
The authors have demonstrated the ability of cord blood–derived unrestricted somatic stem cells (USSCs) and gelatin-modified PHBV scaffold to promote skin regeneration. The scaffolds were designed by electrospinning method and evaluated by different analyses. Results of physical and mechanical analyses also were shown a good resilience as a skin graft. In animal model; all groups excluding the control group exhibited the most pronounced effect on wound closure, with the statistically significant...
#1Esmaeil Biazar(IAU: Islamic Azad University)H-Index: 24
Introduction: Potential use of umbilical cord (UC) is one of the most exciting frontiers in medicine for repairing damaged tissues. UC and cord blood-derived stem cells are the world's largest potential sources of stem cells. UC contains a mixture of stem and progenitor cells at different lineage commitment stages and UC has been verified as a candidate for cell-based therapies and tissue engineering applications due to the capability of these cells for extensive self-renewal and multi-lineage c...
: In this study, we have demonstrated the ability of cord blood (CB)-derived unrestricted somatic stem cells (USSCs) and chitosan-modified poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffold to promote skin regeneration. Afterward, the scaffolds were evaluated by structural, microscopic, physical, and mechanical assays and cell culture analyses. Results of structural, physical, and mechanical analyses also showed a good resilience and compliance with movement as a skin graft. Cellular e...
Abstract Despite rapid progress in our understanding of wound pathobiology, transformative developments in the care of acute and chronic cutaneous wounds have been elusive. Thus, patient quality of life remains poor and the economic burden remains enormous. Although several natural and engineered skin substitutes are commercially available, many contain tissue cells or cell remnants, which make quality control difficult, shelf-life under ambient conditions short, or user interface less than frie...
Last. Farzaneh Avani(AUT: Amirkabir University of Technology)H-Index: 1
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Nanofibrous biomaterials, because of their structural similarity to the extracellular matrix, can be employed as a scaffold in tissue engineering. The use of biopolymers to nanofibers fabrication a...
Polymer scaffolds constitute a very interesting strategy for tissue engineering. Even though they are generally non-toxic, in some cases, they may not provide suitable support for cell adhesion, proliferation, and differentiation, which decelerates tissue regeneration. To improve biological properties, scaffolds are frequently enriched with bioactive molecules, inter alia extracellular matrix proteins, adhesive peptides, growth factors, hormones, and cytokines. Although there are many papers des...
We report a water-soluble and non-toxic method to incorporate additional extracellular matrix proteins into gelatin hydrogels, while obviating the use of chemical crosslinkers such as glutaraldehyde. Gelatin hydrogels were fabricated using a range of gelatin concentrations (4%–10%) that corresponded to elastic moduli of approximately 1 kPa–25 kPa, respectively, a substrate stiffness relevant for multiple cell types. Microbial transglutaminase was then used to enzymatically crosslink a layer of l...
: In the last decade, the Islamic Republic of Iran has witnessed significant improvement and growth in the field of interdisciplinary medicine and in its translation to patients, including the field of cell and stem cell therapy. The main aim of this report is to highlight various advances in regenerative medicine for skin and dermatology using stem cell technology, and its translation to clinic in the past two decades, in Iranian academic centers, clinical institutes and hospitals. While there ...
Abstract Emulsion templating is an emerging route for the production of highly porous scaffolds (PolyHIPEs) with interconnected porosity. Polycaprolactone (PCL) is one of the most extensively used synthetic, bioresorbable polymer for scaffold materials for both hard and soft tissues. PolyHIPEs have previously been shown to be challenging to formulate from PCL due to the high viscosity of the polymer, which limits the efficient mixing of the two phases of the emulsion. Herein, we present the deve...
#2Hossein Ghanbari(Tehran University of Medical Sciences)H-Index: 22
Last. Reza Faridi-Majidi(Tehran University of Medical Sciences)H-Index: 19
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Full-thickness skin defect is one of the main clinical problems, which cannot be repaired spontaneously. The aim of this study was to evaluate the feasibility of combining nanofibers with ADM as a bilayer scaffold for treatment of full-thickness skin wounds in a single-step procedure. The nanofibrous polycaprolactone/fibrinogen scaffolds were fabricated by electrospinning. Subsequently, mesenchymal stem cells were isolated from rat adipose tissues and characterized by flow cytometry. Cell adhesi...
#1Arian Ehterami(Shahroud University of Medical Sciences)H-Index: 13
#2Majid Salehi(Shahroud University of Medical Sciences)H-Index: 20
Last. Arash Goodarzi(Tehran University of Medical Sciences)H-Index: 9
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Abstract In the current study, insulin delivering chitosan nanoparticles were coated onto the electrospun poly (e-caprolactone) (PCL)/Collagen (COLL) to produce a potential wound care material. Electrospun matrices were fabricated from PCL/COLL (1:1 (w/w)) solution. The insulin-loaded chitosan nanoparticles were produced by ionic gelation process and then attached onto the yarns. The dressings were investigated regarding their surface wettability, microstructure, the capacity to absorb water, wa...
#2Nasim Annabi(MIT: Massachusetts Institute of Technology)H-Index: 60
Last. Azadeh Montaseri(Tabriz University of Medical Sciences)H-Index: 13
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AbstractThe tissue engineering field has developed in response to the shortcomings related to the replacement of the tissues lost to disease or trauma: donor tissue rejection, chronic inflammation and donor tissue shortages. The driving force behind the tissue engineering is to avoid the mentioned issues by creating the biological substitutes capable of replacing the damaged tissue. This is done by combining the scaffolds, cells and signals in order to create the living, physiological, three-dim...