Optimisation of a simple method to transiently transfect a CHO cell line in high-throughput and at large scale
Abstract
Transient expression of heterologous proteins in mammalian systems is a powerful way to generate protein reagents quickly. However, it has historically suffered from poor yields in comparison to methods where the recombinant gene is stably integrated into the genome and high expressing clones isolated. Transient methods have been well described for HEK-based systems. In this paper we show the use of a design of experiments (DoE) approach to...
Paper Details
Title
Optimisation of a simple method to transiently transfect a CHO cell line in high-throughput and at large scale
Published Date
Dec 1, 2015
Volume
116
Pages
113 - 119
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