In Vitro Cellular & Developmental Biology – Animal
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#1Siti Nurnasihah Md Hashim (Universiti Sains Malaysia)H-Index: 3
#2Muhammad Fuad Hilmi Yusof (Universiti Sains Malaysia)H-Index: 3
Last. Ahmad Azlina (Universiti Sains Malaysia)H-Index: 10
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The nuclear factor of activated T-cell (NFAT) signaling pathway is involved in angiogenesis following initiation by vascular endothelial growth factor (VEGF). A number of angiogenic genes have been associated with calcineurin in the NFAT pathway, forming a calcineurin-NFAT pathway. This study aims to investigate the involvement of four angiogenic genes within the calcineurin-NFAT pathway in the endothelial-like differentiation of stem cells from human exfoliated deciduous teeth (SHED) cultured o...
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#1Valentina V Nenasheva (Kurchatov Institute)
#2Irina V Makarova (Kurchatov Institute)
Last. V. Z. Tarantul (Kurchatov Institute)H-Index: 1
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Template activating factor-I (TAF-I) is a multifunctional protein involved in various biological processes including the inhibition of histone acetylation, DNA replication, cell cycle regulation, and oncogenesis. Two main TAF-I isoforms with different N-termini, TAF-Iα and TAF-Iβ (SET), are expressed in cells. There are numerous data about functional properties of TAF-Iβ, whereas the effects of TAF-Iα remain largely unexplored. Here, we employed focus formation and cell proliferation assays, TUN...
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#1Farruk M. Lutful Kabir (AU: Auburn University)H-Index: 6
#2Patricia DeInnocentes (AU: Auburn University)H-Index: 13
Last. R. Curtis Bird (AU: Auburn University)H-Index: 13
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The cyclin-dependent kinase inhibitors (CKIs) belong to a group of key cell cycle proteins that regulate important cancer drug targets such as the cyclin/CDK complexes. Gene defects in the INK4A/B CKI tumor suppressor locus are frequently associated with human cancers and we have previously identified similar defects in canine models. Many of the cancer-associated genetic alterations, known to play roles in mammary tumor development and progression, appear similar in humans and dogs. The objecti...
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#1Hua Xia (CQMU: Chongqing Medical University)H-Index: 2
#2Yamin Liu (KIZ: Kunming Institute of Zoology)
Last. Yajun Xie (CQMU: Chongqing Medical University)H-Index: 4
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Metastasis is inevitable in about 30% of patients with primary renal cell carcinoma after nephrectomy treatment. APOBEC1 complementation factor (A1CF), an RNA binding protein, participates in tumor progressions such as growth, apoptosis, differentiation, and invasion. Here, we explored biological functions of A1CF and provided a new insight into renal cell carcinoma metastasis. Wound healing assay was conducted to detect migration in A1CF overexpression and knockdown stable cell lines. Quantitat...
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LIM kinases are involved in various cellular events such as migration, cycle, and differentiation, but whether they have a role in the specification of mammalian early endoderm remains unclear. In the present study, we found that depletion of LIMK2 severely inhibited the generation of definitive endoderm (DE) from human embryonic stem cells (hESCs) and promoted an early neuroectodermal fate. Upon the silencing of LIMK2 during the endodermal differentiation, the assembly of actin stress fibers wa...
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#1Bo Pan (Dalian Medical University)H-Index: 1
#2Dongyi Zhao (Dalian Medical University)H-Index: 1
Last. Zuowei Zhao (Dalian Medical University)H-Index: 11
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Malignant pleural effusion (MPE) presents a severe medical condition in patients with advanced breast cancer (BC). We applied organoid culture technology to culture preoperative puncture specimen and corresponding surgical specimen-derived tumor cells from early BC patients and pleural effusion-derived tumor cells from advanced BC patients with MPE to study whether in vitro models could predict therapies of clinical patients. We successfully expanded pleural effusion-derived tumor organoids from...
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#1Yuefan Song (Dalian Ocean University)H-Index: 9
#2Yi QuH-Index: 3
Last. Qi Yang (SJTU: Shanghai Jiao Tong University)H-Index: 4
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Sponges are among the most primitive multicellular organisms and well-known as a major source of marine natural products. Cultivation of sponge cells has long been an attractive topic due to the prominent evolutionary and cytological significance of sponges and as a potential approach to supply sponge-derived compounds. Sponge cell culture is carried out through culturing organized cell aggregates called 'primmorphs.' Most research culturing sponge cells has used unfractionated cells to develop ...
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#1Luis Daniel Goyzueta Mamani (UFPR: Federal University of Paraná)H-Index: 2
#2Júlio Cesar de Carvalho (UFPR: Federal University of Paraná)H-Index: 16
Last. Carlos Ricardo Soccol (UFPR: Federal University of Paraná)H-Index: 60
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This study presents an in vitro evaluation of the antitumor potential of a chitin-like exopolysaccharide (EPS, produced by Mortierella alpina) on Adrenocortical carcinoma cells (ACC) compared to mitotane, a commercial drug commonly used in ACC treatment, and known for its side effects. Techniques of cellular viability determination such as MTT and fluorescence were used to measure the cytotoxic effects of the EPS and mitotane in tumoral cells (H295R) and non-tumoral cells (VERO), observing high ...
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#1Shiva Pratap Singh (ICAR: Indian Council of Agricultural Research)H-Index: 1
#2Suresh Dinkar Kharche (ICAR: Indian Council of Agricultural Research)H-Index: 7
Last. Manmohan Singh Chauhan (NDRI: National Dairy Research Institute)H-Index: 12
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Top fields of study
Molecular biology
Stem cell
Biochemistry
Cell culture
Biology
Cell biology