Abstract In this study, the characteristic patterns of ferroptosis in clear cell renal cell carcinoma (ccRCC) were systematically investigated, with the interactions between ferroptosis and the tumor microenvironment (TME). On the mRNA expression profiles of 57 ferroptosis-related genes (FRGs), three ferroptosis patterns were constructed, with distinct prognosis and immune cell infiltrations (especially T cells and dendritic cells). The high ferroptosis scores were characterized by poorer progno...
Last. Raman Bahal(UConn: University of Connecticut)H-Index: 19
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Peptide nucleic acids (PNAs), a synthetic DNA mimic, have been extensively utilized for antisense- and antigene-based biomedical applications. Significant efforts have been made to increase the cellular uptake of PNAs, but here we examined relatively unexplored aspects of intracellular trafficking and endocytic recycling of PNAs. For proof-of-concept, we used anti-microRNA (miR) PNA targeting miR-155. The sub-cellular localization of PNA was studied via confocal and flow-cytometry-based assays i...
Last. Shiwei Duan(NBU: Ningbo University)H-Index: 38
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Abstract miR-940 is a microRNA located on chromosome 16p13.3, which has varying degrees of expression imbalance in many diseases. It binds to the 3'untranslated region (3'UTR) and affects the transcription or post-transcriptional regulation of target protein-coding genes. For a diversity of cellular processes including cell proliferation, migration, invasion, apoptosis, EMT, cell cycle, and osteogenic differentiation, miR-940 can affect them not only by regulating protein-coding genes but also L...
Last. Fang Wang(SJTU: Shanghai Jiao Tong University)H-Index: 5
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Abstract Postischemic myocardial fibrosis is a factor for the development of cardiac dysfunction and malignant cardiac arrhythmias, and no effective therapy is currently available. Circular RNAs are emerging as important epigenetic players in various biological functions; however, their roles in cardiac fibrosis are unknown. Using a rat model of postischemic myocardial fibrosis, we identified an increase in circHNRNPH1 in the ischemic myocardium after myocardial infarction, particularly in cardi...
#1Hengzhi Du(HUST: Huazhong University of Science and Technology)H-Index: 2
#2Zhongwei Yin(HUST: Huazhong University of Science and Technology)H-Index: 15
Last. Dao Wen Wang(HUST: Huazhong University of Science and Technology)H-Index: 62
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Abstract null null A variety of studies indicate that microRNAs (miRNAs) are involved in diabetes. However, the direct role of miR-320a in the pathophysiology of pancreatic β cells under diabetes mellitus remains unclear. In the current study, islet transplantation and hyperglycemic clamp assays were performed in miR-320a transgenic mice to explore the effects of miR-320a on pancreatic β cells in vivo. Meanwhile, β cell-specific overexpression or inhibition of miR-320a was delivered by adeno-ass...
Abstract null null Zinc-finger nucleases (ZFNs), transcription activator-like endonucleases (TALENs) and CRISPR-associated Cas9 endonucleases are three major generations of genome editing tools. However, no parallel comparison about the efficiencies and off-target activity of the three nucleases has been reported, which is critical for the final clinical decision. We for the first time developed the GUIDE-seq method in ZFNs and TALENs with novel bioinformatic algorithms to evaluate the off-targe...
Abstract null Autosomal dominant optic atrophy (DOA) is the most common inherited optic neuropathy in the United Kingdom. DOA has an insidious onset in early childhood, typically presenting with bilateral, central visual loss caused by the preferential loss of retinal ganglion cells. 60-70% of genetically confirmed DOA cases are associated with variants in OPA1, a ubiquitously expressed, GTPase that regulates mitochondrial homeostasis through coordination of inner membrane fusion, maintenance of...
Last. Ruben Artero(University of Valencia)H-Index: 24
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Abstract null Skeletal muscle symptoms strongly contribute to mortality of myotonic dystrophy type 1 (DM1) patients. DM1 is a neuromuscular genetic disease caused by CTG repeat expansions that, upon transcription, sequester the Muscleblind-like family of proteins and dysregulate alternative splicing of hundreds of genes. However, mis-splicing does not satisfactorily explain muscle atrophy and wasting, and several other contributing factors have been suggested, including hyperactivated autophagy ...
Abstract null null Doxorubicin (DOX)-induced cardiotoxicity has been one of the major limitations for its clinical use. Although extensive studies have been conducted to decipher the molecular mechanisms underlying DOX cardiotoxicity, no effective preventive or therapeutic measures have yet been identified. Microarray analysis showed that multiple lncRNAs are differentially expressed between control and doxorubicin treated cardiomyocytes. Functional enrichment analysis indicated that the differe...
Abstract null null After angiogenesis-activated embryonic and early postnatal vascularization, endothelial cells (ECs) in most tissues enter a quiescent state necessary for proper tissue perfusion and EC functions. Notch signaling is essential for maintaining EC quiescence, but the mechanisms of action remain elusive. Here, we show that miR-218 is a downstream effector of Notch in quiescent ECs. Notch activation upregulated, while Notch blockade downregulated, miR-218 and its host gene Slit2, li...