Molecular therapy. Nucleic acids
Papers 1,789
1 page of 179 pages (1,789 results)
#1Christopher Barnes (UCB: University of California, Berkeley)H-Index: 1
#2Hyun-Cheol Lee (QB3)H-Index: 8
Last. David V. SchafferH-Index: 82
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We describe a genome-wide screening strategy to identify target genes whose modulation increases the capacity of a cell to produce recombinant adeno-associated viral (AAV) vector. Specifically, a single-guide RNA (sgRNA) library for a CRISPR-based genome-wide transcriptional activation screen was inserted into an AAV vector, and iterative rounds of viral infection and rescue in HEK293 producer cells enabled the enrichment of sgRNAs targeting genes whose upregulation increased AAV production. Num...
#1Yue Chang (NU: Nanjing University)H-Index: 2
#2Yulu Zhao (NMU: Nanjing Medical University)H-Index: 2
Last. Bibo Wang (NU: Nanjing University)
view all 22 authors...
Dysregulated alternative splicing (AS) plays critical roles in driving cancer progression, and the underlying mechanisms remain largely unknown. Here, we demonstrated that PHF5A, a component of U2 small nuclear ribonucleoproteins, was frequently upregulated in colorectal cancer (CRC) samples and associated with poor prognosis. PHF5A promoted proliferation and metastasis of CRC cells in vitro and in vivo. Transcriptomic analysis identified PHF5A-regulated AS targets and pathways. Particularly, PH...
#1Yongpeng Li (SZU: Shenzhen University)
#2Yanan Tan (CAS: Chinese Academy of Sciences)
Last. Qifang Lei (SZU: Shenzhen University)H-Index: 3
view all 11 authors...
Triple-negative breast cancer (TNBC) is an aggressive and highly lethal disease. The lack of targeted therapies and poor patient outcome have fostered efforts to discover new molecular targets to treat patients with TNBC. Here, we showed that baculoviral IAP repeat containing 6 (BIRC6) is overexpressed and positively correlated with epidermal growth factor (EGF) receptor (EGFR) in TNBC cells and tissues and that BIRC6 overexpression is associated with poor patient survival. Mechanistic studies r...
A major change in the transcriptome of testicular Sertoli cells (Scs) at the onset of puberty enables them to induce robust spermatogenesis. Through comprehensive literature mining, we generated a list of genes crucial for Sc functioning and computationally predicted the microRNAs regulating them. Differential expression analysis of microRNAs in infant and pubertal rat Scs showed that miR-382-3p levels decline significantly in pubertal Scs. Interestingly, miR-382-3p was found to regulate genes l...
#1Dawei Rong (SEU: Southeast University)
#2Fan Wu (NMU: Nanjing Medical University)H-Index: 4
Last. Jinren Zhou (NMU: Nanjing Medical University)
view all 13 authors...
Abstract null N6-methyladenosine (m6A) is capable of mediating circRNA generation in carcinoma biology. Nevertheless, the posttranscriptional systems of m6A and circRNA in hepatocellular carcinoma (HCC) development are still unclear. The present study identified a circRNA with m6A modification, circHPS5, which was increased in neoplasm HCC tissues and indicated poor patient survival. Silencing of circHPS5 inhibited epithelial-mesenchymal transition (EMT) and cancer stem-like cell (CSC) phenotype...
#1Xue Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 1
#2Peng Wang (SJTU: Shanghai Jiao Tong University)H-Index: 6
Last. Wei Liang (SJTU: Shanghai Jiao Tong University)H-Index: 7
view all 8 authors...
Abstract null Arteriosclerosis obliterans (ASO) of the lower extremities is identified as a kind of cardiovascular disease with aberrant proliferation and apoptosis of vascular smooth muscle cells (VSMCs). Accumulating studies have demonstrated the vital role of Yes1 associated transcriptional regulator (YAP1) in VSMCs, while its upstream regulatory mechanism in VSMCs in ASO of the lower extremities needs to be further elucidated. Herein, hsa_circ_0024093, a circular RNA (circRNA) from YAP1, was...
#1Ding Lei (Jilin University)
#2Taiyuan Liu (Jilin University)
Last. Shanyong Zhang (Jilin University)
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Osteosarcoma is a highly aggressive cancer common in children and adolescents. There is still a lack of effective treatments for metastatic or recurrent osteosarcoma. The role of long non-coding RNAs (lncRNAs) in osteosarcoma has gradually attracted attention. Here, we identified lncRNAs that were abnormally expressed in metastatic osteosarcoma through analyzing the sequencing data of osteosarcoma tissues and selected upregulated lncRNA MELTF-AS1 for detailed study. The qRT-PCR analysis showed t...
#1Masayuki Sano (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 12
#2Kana Morishita (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 5
Last. Yoshio Kato (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 27
view all 6 authors...
Abstract null null MicroRNAs (miRNAs) are small noncoding RNAs that regulate complex gene expression networks in eukaryotic cells. Because of their unique expression patterns, miRNAs are potential molecular markers for specific cell states. Although a system capable of imaging miRNA in living cells is needed for visually detecting miRNA expression, very few fluorescence signal-on sensors that respond to the expression of target miRNA (miR-ON sensor) are available. Here, we report an miR-ON senso...
#1Jie Pan (SU: Stanford University)
#2Nana MaH-Index: 4
Last. Wei ZhangH-Index: 46
view all 6 authors...
Blood-brain barrier (BBB) dysfunction is associated with an accumulation of neurotoxic molecules and increased infiltration of peripheral cells within the brain parenchyma. Accruing evidence suggests that microglia and astrocytes play a crucial role in the recovery of BBB integrity and the corralling of infiltrating cells into clusters after brain damage, but the mechanisms involved remain unclear. Intriguingly, the results of flow cytometry and immunofluorescence analyses have shown that BBB pe...
#1Androulla N. Miliotou (Aristotle University of Thessaloniki)H-Index: 3
#2Ioannis S. Pappas (University of Thessaly)H-Index: 14
Last. Lefkothea C. Papadopoulou (Aristotle University of Thessaloniki)H-Index: 12
view all 7 authors...
The potential clinical applications of the powerful in vitro-transcribed (IVT)-mRNAs, to restore defective protein functions, strongly depend on their successful intracellular delivery and transient translation through the development of safe and efficient delivery platforms. In this study, an innovative (international patent-pending) methodology was developed, combining the IVT-mRNAs with the protein transduction domain (PTD) technology, as an efficient delivery platform. Based on the PTD techn...
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