Methods of Molecular Biology
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#1Flavia Pedrini (UFSCar: Federal University of São Carlos)
#2Moema Hausen (PUC-SP: Pontifícia Universidade Católica de São Paulo)H-Index: 7
Last. Eliana A R Duek (UFSCar: Federal University of São Carlos)
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Bioreactor systems that allow the simulation of in vivo variables in a controlled in vitro environment, were a great advance in the field of tissue engineering. Due to the dynamic-mechanical features that some tissues present, 3D-engineered constructs often do not exhibit the biomechanical properties of these native tissues. Thus, a successful approach must not only achieve tissue repair but also restore its function after injury. Here, we describe a method to improve cell activity in 3D scaffol...
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#1Mohammad Izadifar (Lunenfeld-Tanenbaum Research Institute)H-Index: 9
#2Tünde BereczH-Index: 2
Last. Andras Nagy (Lunenfeld-Tanenbaum Research Institute)H-Index: 106
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Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) hold great promise for cardiovascular disease modeling, drug screening and personalized medicine. A crucial requirement to establish an hPSC-CM-based disease model is the availability of a reliable differentiation protocol and a functional assessment of phenotypic properties of CMs in a disease context. Characterization of relative changes in contractile behavior of CMs can provide insight not only about drug effects but into the path...
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#1Xi Yao (French Institute of Health and Medical Research)H-Index: 5
#2Christian Dani (French Institute of Health and Medical Research)H-Index: 67
Beige/brite/brown-like adipocytes (BAs), dispersed in white adipose tissue, represent promising cell targets to counteract obesity and associated diseases. However, there are major limitations for a BA-based treatment of obesity, among which the main ones are the rareness of BAs in adult humans and the lack of a relevant cell culture condition for modeling the development of BAs. We describe in this chapter the capacity of human induced pluripotent stem cells-derived BA progenitors (hiPSC-BAPs) ...
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#1Álvaro Mourenza (University of León)H-Index: 4
#2José A. Gil (University of León)H-Index: 31
Last. Michal Letek (University of León)H-Index: 19
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Antibiotherapy is the main therapeutic strategy in the fight against bacterial pathogens. However, the misuse of antimicrobials has led to the appearance of antimicrobial-resistant strains. The rate at which we isolate multidrug-resistant bacteria is now much faster than the discovery rate of new antimicrobials. Therefore, the repurposing of approved drugs against multidrug-resistant bacteria is a very promising strategy to find new therapies against these pathogens. Some antibiotics generate ox...
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Antibiotic resistance is not only a European or American problem: it is a global crisis. The emergence of resistance to multiple antimicrobial agents has become a major threat to public health. The main objective of the institution's antimicrobial policy is to adapt the effective treatment to each patient, with a minimum of complications, avoid adverse reactions, control the development and spread of strains of resistant microorganisms as well as reduce hospital costs whenever possible. World He...
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#1Hasan Yuksel (Celal Bayar University)H-Index: 24
#2Merve Öcalan (Celal Bayar University)
The epithelial barrier is the basic unit that ensures the continuation of life for all living things. It provides separation of living cells or organelles from nature and microenvironment. Thus, life and functions continue. It is the same for the human organism. However, the normal properties of this epithelial barrier may differ in each organ and tissue. The two most important barriers that separate humans from nature and their microenvironment are the respiratory tract and the gastrointestinal...
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#1O. F. Gordeeva (RAS: Russian Academy of Sciences)H-Index: 8
The 3D embryoid body (EB) differentiation model is a promising tool for fundamental cell biology and drug discovery studies assessing the compound effects on mammalian and human development. This 3D cell model allows for analyzing spatiotemporal changes during morphogenesis and differentiation. A combination of confocal microscopy with high content image analysis (HCIA) can significantly improve the study of spatiotemporal patterns of early embryonic lineages and compound efficacy and toxicity t...
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#1Shermaine Huiping Tay (Agency for Science, Technology and Research)
#2Winanto (Agency for Science, Technology and Research)H-Index: 3
Last. Shi-Yan Ng (Agency for Science, Technology and Research)H-Index: 17
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The use of patient-derived induced pluripotent stem cells (iPSCs) and their neural derivatives is becoming increasingly important in the study of neurodegenerative disorders such as Alzheimer's disease, Parkinson's disease, Lewy body dementia, amyotrophic lateral sclerosis, peripheral neuropathy, and so on. Increasingly, iPSC-derived neurons also reveal key pathways and signaling defects in psychiatric disorders such as autism spectrum disorders, schizophrenia, and bipolar disorder. With recent ...
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#1Marites T. Woon (Lonza Group)H-Index: 1
#2Puspa R. Pandey (Lonza Group)H-Index: 13
Last. Inbar Friedrich Ben-Nun (Lonza Group)H-Index: 1
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Bioreactor technolology enables the expansion of mammalian cells, which can be translated to processes compatible with Current Good Manufacturing Practice (cGMP) regulations. Cells are introduced to the bioreactor vessel, wherein key parameters such as temperature, pH, and oxygen levels are tightly controlled to facilitate growth over time. Here, we describe the microcarrier-based expansion of human pluripotent stem cells in a 3 L stirred tank bioreactor.
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#1Vlasta Lungova (UW: University of Wisconsin-Madison)H-Index: 5
#2Susan L. Thibeault (UW: University of Wisconsin-Madison)H-Index: 30
Healthy vocal fold mucosa is composed of two major cell types, non-keratinized stratified squamous epithelium and vocal fold fibroblasts. Although dysfunction of the epithelium may play a significant pathogenic role in vocal fold diseases, studies at the genetic and molecular level using primary epithelial cells or models of human vocal fold mucosa have been significantly limited by the availability of relevant tissue types, poor growth, and heterogeneity of primary vocal fold epithelial cells. ...
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