Methods of Molecular Biology
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#1Marie Zufferey (SIB: Swiss Institute of Bioinformatics)H-Index: 3
#2Daniele Tavernari (SIB: Swiss Institute of Bioinformatics)H-Index: 7
Last. Giovanni Ciriello (SIB: Swiss Institute of Bioinformatics)H-Index: 32
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Chromatin folding in the 3D space of the nucleus can be explored through high-throughput chromosome conformation capture (Hi-C) approaches. These experiments quantify the number of interactions between any pair of genomic loci in the genome and, thus, allow building genome-scale maps of intra- and inter-chromosomal contacts (contact maps). Statistical and algorithmic analyses of Hi-C data consist in extracting information from these contact maps. One of the most striking patterns observed in int...
#1Xu Zhang (Duke University)H-Index: 13
#2Nadia O. Abutaleb (Duke University)H-Index: 4
Last. George A. Truskey (Duke University)H-Index: 50
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Human tissue-engineered blood vessels (TEBVs) that exhibit vasoactivity can be used to test drug toxicity, modulate pro-inflammatory cytokines, and model disease states in vitro. We developed a novel device to fabricate arteriole-scale human endothelialized TEBVs in situ with smaller volumes and higher throughput than previously reported. Both primary and induced pluripotent stem cell (iPSC)-derived cells can be used. Four collagen TEBVs with 600μm inner diameter and 2.9 mm outer diameter are fa...
#1Adam W. Kiefer (UNC: University of North Carolina at Chapel Hill)H-Index: 21
#2Cortney Armitano-Lago (UNC: University of North Carolina at Chapel Hill)H-Index: 1
Last. Paula L. Silva (UC: University of Cincinnati)H-Index: 17
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The best predictor of future injury is previous injury and this has not changed in a quarter century despite the introduction of evidence-based medicine and associated revisions to post-injury treatment and care. Nearly nine million sports-related injuries occur annually, and the majority of these require medical intervention prior to clearance for the athlete to return to play (RTP). Regardless of formal care, these athletes remain two to four times more likely to suffer a second injury for sev...
#2Obdulia RabalH-Index: 19
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The discovery and development of drugs is a long and expensive process with a high attrition rate. Computational drug discovery contributes to ligand discovery and optimization, by using models that describe the properties of ligands and their interactions with biological targets. In recent years, artificial intelligence (AI) has made remarkable modeling progress, driven by new algorithms and by the increase in computing power and storage capacities, which allow the processing of large amounts o...
#1Yuhong Han (The University of Texas Southwestern Medical Center)H-Index: 7
#2Jin Jiang (The University of Texas Southwestern Medical Center)H-Index: 40
The Hedgehog (Hh) family of secreted proteins governs embryonic development and adult tissue homeostasis by regulating the abundance, localization, and activity of the GPCR family protein Smoothened (Smo). Smo trafficking and subcellular accumulation are controlled by multiple posttranslational modifications (PTMs) including phosphorylation, ubiquitination, and sumoylation, which appears to be conserved from Drosophila to mammals. Smo ubiquitination is dynamically regulated by E3 ubiquitin ligas...
#1Sheikh I. Hossain (UTS: University of Technology Sydney)H-Index: 5
#2Mohammad Zohurul Islam (UTS: University of Technology Sydney)
Last. Evelyne Deplazes (UTS: University of Technology Sydney)H-Index: 12
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The lung surfactant monolayer (LSM) is a thin layer of lipids and proteins that forms the air/water interface of the alveoli. The primary function of the LSM is to reduce the surface tension at the air/water interface during breathing. The LSM also forms the main biological barrier for any inhaled particles, including drugs, to treat lung diseases. Elucidating the mechanism by which these drugs bind to and absorb into the LSM requires a molecular-level understanding of any drug-induced changes t...
#1Enrique A Sampedro-Carrillo (UNAM: National Autonomous University of Mexico)H-Index: 2
The basic goal of all histological techniques is the identification of tissue components, both normal and pathologic. In the case of any immunodetection technique we expect: specific detection of the antigen with a mark strong enough to be easily detected; and a clear preservation of histological and cytological details of the environment. To achieve these expectations the technical procedures employed must be clearly understood so we can control the previous methodological steps, avoiding any f...
#1Mario Latendresse (SRI: SRI International)H-Index: 20
#2Wai Kit Ong (SRI: SRI International)H-Index: 7
Last. Peter D. Karp (SRI: SRI International)H-Index: 58
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The MetaFlux software supports creating, executing, and solving quantitative metabolic flux models using flux balance analysis (FBA). MetaFlux offers four modes of operation: (1) solving mode executes an FBA model for an individual organism or for an organism community, (2) gene knockout mode executes an FBA model with one or many gene knockouts, (3) development mode assists the user in creating and improving FBA models, and (4) flux variability analysis mode generates a report of the robustness...
#1Caleb Jensen (GRU: Augusta University)H-Index: 2
#2Chloe Shay (Emory University)H-Index: 12
Last. Yong Teng (GRU: Augusta University)H-Index: 30
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In vitro cancer research models require the utmost accuracy and precision to effectively investigate physiological pathways and mechanisms, as well as test the therapeutic efficacy of anticancer drugs. Although two-dimensional (2D) cell culture models have been the traditional hallmark of cancer research, increasing evidence suggests 2D tumor models cannot accurately recapitulate complex aspects of tumor cells and drug responses. Three-dimensional (3D) cell cultures, however, are more physiologi...
#1Lauren A Lowe (UNSW: UNSW Sydney)
#2Daniel W K Loo (UNSW: UNSW Sydney)
Last. Anna Wang (UNSW: UNSW Sydney)
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Fatty acids readily assemble into bilayer membranes at a pH near their apparent pKa. Fatty acid vesicles are not only useful for research in the fields of origins of life, soft matter science, biophysics, and drug delivery, but are also cost-effective and easy to manipulate, making them ideal for teaching students about self-assembly and lipid bilayers. Here, we describe simple ways to make giant, unilamellar fatty acid vesicles suitable for microscopy and encapsulation studies.
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