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#1Marco Tigano (NYU: New York University)H-Index: 6
#1M. Tigano (NYU: New York University)
Last. Agnel Sfeir (NYU: New York University)H-Index: 23
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Mitochondrial double-strand breaks (mtDSBs) are toxic lesions that compromise mitochondrial function. Mito-nuclear communication is essential to maintain cellular homeostasis, however, the nuclear response to mtDSBs remains unknown. Using mitochondrial-targeted TALENs, we show that mtDSBs activate a type I interferon response evidenced by phosphorylation of STAT1 and activation of interferon stimulated genes (ISG). Following mtDNA break formation, BAK-BAX mediated herniation releases mitochondri...
2 CitationsSource
#1Mariko Katoh-Kurasawa (BCM: Baylor College of Medicine)H-Index: 8
Last. Gad Shaulsky (BCM: Baylor College of Medicine)H-Index: 50
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Development of the social amoeba Dictyostelium discoideum begins by starvation of single cells and ends in multicellular fruiting bodies 24 hours later. These major morphological changes are accompanied by sweeping gene expression changes, encompassing nearly half of the 13,000 genes in the genome. To explore the relationships between the transcriptome and developmental morphogenesis, we performed time-series RNA-sequencing analysis of the wild type and 20 mutant strains with altered morphogenes...
#1Eva HitzH-Index: 3
Last. Till S. VossH-Index: 25
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Cyclic AMP (cAMP) signalling is crucial for the propagation of asexual malaria blood stage parasites. Recent work on Plasmodium falciparum demonstrated that phosphorylation of the invasion ligand AMA1 by the catalytic subunit of cAMP-dependent protein kinase A (PfPKAc) is an essential step during parasite invasion into red blood cells. However, the exact mechanisms regulating PfPKAc activity are only partially understood and PfPKAc function has not been extensively studied in gametocytes, the se...
#1Brandon J. Rea (UI: University of Iowa)H-Index: 3
#2Levi P. SowersH-Index: 10
Last. Andrew F. RussoH-Index: 46
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We developed an automated squint assay using both black C57BL/6J and white CD1 mice that measured the interpalpebral fissure area between the upper and lower eyelids as an objective quantification of pain. In C57BL/6J mice, we observed a squint response to increasing doses of a migraine trigger, the neuropeptide CGRP, including a significant response in female mice at a dose below detection by the manual grimace scale. Using the automated software, both C57BL/6J and CD1 mice lacked a detectable ...
#1Christine R. Zheng (Wyss Institute for Biologically Inspired Engineering)
#2Abhyudai Singh (UD: University of Delaware)H-Index: 33
Last. Elizabeth A. Libby (Wyss Institute for Biologically Inspired Engineering)
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A lack of direct single-cell readouts for bacterial kinase activity remains a major barrier to our understanding of most signaling systems. At the single-cell-level, protein kinase activity is typically inferred by the activity of downstream transcriptional reporters. Complicating this approach in vivo, promoters are often co-regulated by several pathways, making the activity of a specific kinase difficult to deconvolve. Here, we have designed and constructed new, direct and specific sensors of ...
#1Jeffrey A. Ruffolo (Johns Hopkins University)H-Index: 2
#2Jeremias SulamH-Index: 4
Last. Jeffrey J. GrayH-Index: 51
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Therapeutic antibodies make up a rapidly growing segment of the biologics market. However, rational design of antibodies is hindered by reliance on experimental methods for determining antibody structures. In recent years, deep learning methods have driven significant advances in general protein structure prediction. Here, we present DeepAb, a deep learning method for predicting accurate antibody FV structures from sequence. We evaluate DeepAb on two benchmark sets - one balanced for structural ...
#1Rawsthorne-Manning H (University of Southampton)
#2Fernando Calahorro (University of Southampton)H-Index: 9
Last. James Dillon (University of Southampton)H-Index: 9
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Multiple advances have been made to increase the efficiency of CRISPR/Cas9 editing using the model genetic organism Caenorhabditis elegans (C. elegans). Here we report on the use of co-CRISPR marker genes: worms in which co-CRISPR events have occurred have overt, visible phenotypes which facilitates the selection of worms that harbour CRISPR events in the target gene. Mutation in the co-CRISPR gene is then removed by outcrossing to wild type but this can be challenging if the CRISPR and co-CRISP...
#1Qiao Yang (WHU: Wuhan University)
Background. The central dogma of radiation biology is that the cytotoxic and mutagenic effects of radiation are principally the result of biological macromolecules (DNA, RNA, polysaccharides, proteins and lipids) damage caused during the course of irradiation. Previous studies on the radioresistance of the extremely radiation-resistant red-carotenoid-pigmented bacterium Deinococcus radiodurans have been focused on DNA repair system. However, relative little is known about the biochemical basis o...
#1Fan L (UCR: University of California, Riverside)
#2Zhang C (CAS: Chinese Academy of Sciences)H-Index: 1
Last. Xi Chen (UCR: University of California, Riverside)H-Index: 122
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Mobile microRNAs (miRNAs) serve as local and long-distance signals in developmental patterning and stress responses in plants. However, mechanisms governing the non-cell autonomous activities of miRNAs remain elusive. Here, we show that mutations that disrupt microtubule dynamics are specifically defective for the non-cell autonomous actions of mobile miRNAs, including miR165/6 that is produced in the endodermis and moves to the vasculature to pattern xylem cell fates in Arabidopsis roots. We sh...
#1Edina Szabo (U of C: University of Calgary)H-Index: 1
Last. Constance A. M. FinneyH-Index: 15
view all 10 authors...
Co-infections are a common reality but understanding how the immune system responds in this context is complex and can be unpredictable. Despite this, it is key to develop models that will provide better translatability to real world situations. Heligmosomoides polygyrus (parasitic roundworm) and Toxoplasma gondii (protozoan parasite) are well studied organisms that stimulate a characteristic Th2 and Th1 response respectively. IFN{gamma}-producing T cells, NK and {gamma}{delta} T cells contribut...
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Computer science
Cell biology