Journal of Visualized Experiments
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#1Weiyan Jiang (WMU: Wenzhou Medical College)
Last. Wang Wei (First Affiliated Hospital of Wenzhou Medical University)
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The mechanisms contributing to the natural onset of cerebral venous sinus thrombosis (CVST) are mostly unknown, and a variety of uncontrollable factors are involved in the course of the disease, resulting in great limitations in clinical research. Therefore, the establishment of stable CVST animal models that can standardize a variety of uncontrollable confounding factors have helped to circumvent shortcomings in clinical research. In recent decades, a variety of CVST animal models have been con...
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#1Raphaëlle Brissette (HEC Montréal)
#2Pierre-Majorique Léger (HEC Montréal)H-Index: 20
Last. Sylvain Sénécal (HEC Montréal)H-Index: 20
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The goal of this methodology is to assess explicit and implicit measures of engagement of spectators during social digital games in a group of participants with motion tracking systems. In the context of games that are not confined within a screen, measuring the different dimensions of engagement such as physiological arousal can be challenging. The focus of the study is made on the spectators of the game and the differences in their engagement according to interactivity. Engagement is measured ...
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#1M. Fethullah Simsek (Cincinnati Children's Hospital Medical Center)H-Index: 2
#2Ertugrul M. Ozbudak (Cincinnati Children's Hospital Medical Center)H-Index: 12
Vertebrate embryos pattern their major body axis as repetitive somites, the precursors of vertebrae, muscle, and skin. Somites progressively segment from the presomitic mesoderm (PSM) as the tail end of the embryo elongates posteriorly. Somites form with regular periodicity and scale in size. Zebrafish is a popular model organism as it is genetically tractable and has transparent embryos that allow for live imaging. Nevertheless, during somitogenesis, fish embryos are wrapped around a large, rou...
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#1Shalhevet Azriel (BIU: Bar-Ilan University)
#2Hadar Bootz (BIU: Bar-Ilan University)
Last. Nissan Yissachar (BIU: Bar-Ilan University)H-Index: 5
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The structure of the gut tissue facilitates close and mutualistic interactions between the host and the gut microbiota. These cross-talks are crucial for maintaining local and systemic homeostasis; changes to gut microbiota composition (dysbiosis) associate with a wide array of human diseases. Methods for dissecting host-microbiota interactions encompass an inherent tradeoff among preservation of physiological tissue structure (when using in vivo animal models) and the level of control over the ...
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#1Amira D. Rghei (U of G: University of Guelph)H-Index: 3
#2Brenna A. Y. Stevens (U of G: University of Guelph)
Last. Sarah K. Wootton (U of G: University of Guelph)H-Index: 19
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Adeno-associated virus (AAV) vectors are among the most clinically advanced gene therapy vectors, with three AAV gene therapies approved for humans. Clinical advancement of novel applications for AAV involves transitioning from small animal models, such as mice, to larger animal models, including dogs, sheep, and nonhuman primates. One of the limitations of administering AAV to larger animals is the requirement for large quantities of high-titer virus. While suspension cell culture is a scalable...
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#1Mario Wenger (University of Salzburg)H-Index: 1
#2Athanasios Bethanis (University of Salzburg)H-Index: 1
Last. Lorenz Aglas (University of Salzburg)H-Index: 8
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Mediator release assays analyze in vitro immunoglobulin E (IgE)-mediated degranulation and secretion of mediators by effector cells, such as mast cells and basophils, upon stimulation with serial dilutions of putative allergens. Therefore, these assays represent an essential tool that mimics the in vivo degranulation process, which occurs upon allergen exposure in sensitized patients or in skin prick tests. Additionally, these assays are usually employed to investigate the allergenic potential o...
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#1Yue-li SunH-Index: 6
#2Gan LiH-Index: 2
Last. Yongjun WangH-Index: 24
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