Journal of Visualized Experiments
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1.35
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#1Anthos Christofides (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 3
#2Carol Cao (Harvard University)
Last. Vassiliki A. Boussiotis (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 70
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The respiratory tract is in direct contact with the outside environment and requires a precisely regulated immune system to provide protection while suppressing unwanted reactions to environmental antigens. Lungs host several populations of innate and adaptive immune cells that provide immune surveillance but also mediate protective immune responses. These cells, which keep the healthy pulmonary immune system in balance, also participate in several pathological conditions such as asthma, infecti...
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#1Hans WienkH-Index: 22
#2Lucia BanciH-Index: 89
Last. Anastassis PerrakisH-Index: 64
view all 8 authors...
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#1Anna Theodossiou (TAMU: Texas A&M University)
#2Linghao Hu (TAMU: Texas A&M University)H-Index: 1
Last. Alex J. Walsh (TAMU: Texas A&M University)H-Index: 19
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Cellular metabolism is the process by which cells generate energy, and many diseases, including cancer, are characterized by abnormal metabolism. Reduced nicotinamide adenine (phosphate) dinucleotide (NAD(P)H) and oxidized flavin adenine dinucleotide (FAD) are coenzymes of metabolic reactions. NAD(P)H and FAD exhibit autofluorescence and can be spectrally isolated by excitation and emission wavelengths. Both coenzymes, NAD(P)H and FAD, can exist in either a free or protein-bound configuration, e...
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#1Lichen Zhang (XXMU: Xinxiang Medical University)H-Index: 10
#2Rong Huang (XXMU: Xinxiang Medical University)H-Index: 4
Last. Yinming Liang (XXMU: Xinxiang Medical University)H-Index: 11
view all 10 authors...
Functional genomics studies of the immune system require genetic manipulations that involve both deletion of target genes and addition of elements to proteins of interest. Identification of gene functions in cell line models is important for gene discovery and exploration of cell-intrinsic mechanisms. However, genetic manipulations of immune cells such as T cells and macrophage cell lines using CRISPR/Cas9-mediated knock-in are difficult because of the low transfection efficiency of these cells,...
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#1Yue Zhang (BUCM: Beijing University of Chinese Medicine)H-Index: 2
#2Peng Cao (BUCM: Beijing University of Chinese Medicine)H-Index: 2
Last. Huihua Qu (BUCM: Beijing University of Chinese Medicine)H-Index: 3
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Membrane-based lateral flow immunochromatographic strips (ICSs) are useful tools for low-cost self-diagnosis and have been efficiently applied to toxin, physiological index and clinical biomarker detection. In this protocol, we provide a detailed description of the steps to develop a rapid, sensitive and quantitative lateral-flow immunoassay (using AuNPs as a marker and mAbs as a probe). The procedure describes the preparation and characterization of colloidal gold, synthesis of the AuNP-mAb con...
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#1Ayushi Verma (IITB: Indian Institute of Technology Bombay)H-Index: 2
#2Vipin Kumar (IITB: Indian Institute of Technology Bombay)H-Index: 9
Last. Sanjeeva Srivastava (IITB: Indian Institute of Technology Bombay)H-Index: 30
view all 5 authors...
Recent advances in mass spectrometry have resulted in deep proteomic analysis along with the generation of robust and reproducible datasets. However, despite the considerable technical advancements, sample preparation from biospecimens such as patient blood, CSF, and tissue still poses considerable challenges. For identifying biomarkers, tissue proteomics often provides an attractive sample source to translate the research findings from the bench to the clinic. It can reveal potential candidate ...
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#1Brooke Burris (WUSTL: Washington University in St. Louis)H-Index: 1
#2Nicholas O. Jensen (WUSTL: Washington University in St. Louis)
Last. Mayssa H. Mokalled (WUSTL: Washington University in St. Louis)H-Index: 11
view all 3 authors...
Due to their renowned regenerative capacity, adult zebrafish are a premier vertebrate model to interrogate mechanisms of innate spinal cord regeneration. Following complete transection of their spinal cord, zebrafish extend glial and axonal bridges across severed tissue, regenerate neurons proximal to the lesion, and regain their swim capacities within 8 weeks of injury. Recovery of swim function is thus a central readout for functional spinal cord repair. Here, we describe a set of behavioral a...
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#1Takehiro Kazama (NIES: National Institute for Environmental Studies)H-Index: 2
#2Kazuhide HayakawaH-Index: 14
Last. Akio Imai (NIES: National Institute for Environmental Studies)H-Index: 21
view all 4 authors...
Fast repetition rate fluorometer (FRRf) is a beneficial method for measuring photosystem II (PSII) photophysiology and primary productivity. Although FRRf can measure PSII absorption cross-section (σPSII), maximum photochemical efficiency (Fv/Fm), effective photochemical efficiency (Fq'/Fm'), and non-photochemical quenching (NPQNSV) for various eukaryotic algae and cyanobacteria, almost all FRRf studies to date have focused on phytoplankton. Here, the protocol describes how to measure PSII photo...
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#1Ning Ma (Sun Yat-sen University)H-Index: 5
#2Zhen Zong (NCU: Nanchang University)H-Index: 6
Last. Taicheng Zhou (Sun Yat-sen University)H-Index: 7
view all 9 authors...
Laparoscopic transabdominal preperitoneal hernia repair (TAPP) is one of the most widely used methods in inguinal hernia surgery. After the mesh is placed, the peritoneum must be resutured to avoid contact with the tissues and organs in the abdominal cavity. If the peritoneal suture time is too long, the operation and anesthesia time will be prolonged, increasing the burden on the patient. Moreover, improper suture methods cause serious consequences, such as intestinal obstruction and mesh infec...
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