#1Divya Sahu(UCSD: University of California, San Diego)H-Index: 4
#2Jianya Huan(OHSU: Oregon Health & Science University)
Last. Donna E. Hansel(OHSU: Oregon Health & Science University)H-Index: 4
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Bladder cancer invasion depends on mammalian target of rapamycin complex-2 (mTORC2) activity, although the downstream mTORC2 effectors that mediate this effect have not been fully defined. One potential downstream effector is the arginine derivative nitric oxide (NO). Here, we identified a stage-associated increase in the expression of NO-generating enzymes endothelial NOS (eNOS) and inducible NOS (iNOS) in human bladder cancer. Reduction of NOS activity by pharmacological inhibition or silencin...
Transdifferentiation (or activation) of hepatic stellate cells (HSCs) to myofibroblasts is a key event in liver fibrosis, and activated HSCs in tumor microenvironment reportedly promote tumor progression. This study aimed to analyze the effect of an inhibitor of HSC activation, R-III (a retinol-binding protein - albumin domain III fusion protein), on HSC pro-tumorigenic functions. Although conditioned medium (CM) collected from activated HSCs enhanced the migration, invasion, and proliferation o...
Abstract: null null The lacrimal glands are highly susceptible to aging and exhibit age-related structural and functional alterations. However, the mechanisms by which aging affects the lacrimal glands are not well-established. Here, this study explores the crosstalk between the aging process, gut microbiota, and circadian rhythm in age-associated lacrimal gland dysfunction. C57BL/6J mice were divided into young, old, and fecal microbiota transplant (FMT)-treated old groups. The gut bacterial co...
#1Łukasz Zadka(Wrocław Medical University)H-Index: 3
#2Igor Buzalewicz(Wrocław University of Technology)H-Index: 10
Last. Piotr Dziegiel(Wrocław Medical University)H-Index: 30
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3D imaging and quantitative analysis of extracellular vesicles (EVs) remain largely unexplored mainly due to limitations in detection techniques. In this study, EVs from patients diagnosed with colorectal cancer (CRC) and ulcerative colitis (UC) were examined. To investigate the spatial heterogeneity and 3D refractive index (RI) distributions of single EVs, a label-free digital holographic tomography (DHT) technique was used at a submicrometer spatial resolution. The presented image-processing a...
Last. Jonas Nilsson(University of Gothenburg)H-Index: 54
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Angiogenesis supplies oxygen and nutrients to growing tumors. Inhibiting angiogenesis may stop tumor growth, but VEGF inhibitors have limited effect in most tumors. The limited effect may be explained by an additional, less VEGF-driven, form of angiogenesis known as intussusceptive angiogenesis. The importance of intussusceptive angiogenesis in human tumors is not known. Epifluorescence and confocal microscopy was used to visualize intravascular pillars, the hallmark structure of intussusceptive...
Aquaporins (AQPs) are normally expressed in the corneal epithelium. The aim of this study was to determine whether AQP5 played a role in corneal epithelial wound healing. AQP5 knockout (AQP5-/-) mice were constructed using CRISPR/Cas9 technology. A corneal wound healing model was performed using epithelial debridement on corneas. The time to corneal epithelial and nerve regeneration was significantly delayed in the AQP5-/- mice. Reduced Ki-67 positive cells and nerve growth factor (NGF) expressi...
Hyaluronan (HA)-binding protein involved in HA depolymerization (HYBID) is involved in cartilage destruction via HA depolymerization in human knee osteoarthritis. However, roles of HYBID in the progression of osteoarthritis remain elusive. This study sought to examine whether genetic depletion of Hybid could suppress surgically induced osteoarthritis of mouse knee joints. In osteoarthritis induced by medial collateral ligament transection with meniscus removal, articular cartilage destruction an...
Abstract null null Myelodysplastic syndromes (MDS) are clonal neoplasms of the hematopoietic stem cell that result in aberrant differentiation of hematopoietic lineages due to a wide range of underlying genetic, epigenetic, and other causes. Despite the myriad etiologies, there is a recognizable MDS phenotype that has been associated with microRNA (miRNA) aberrant expression. A model of aberrant myeloid maturation mimicking MDS that is seen in MDS has been created using a stable knockdown of miR...
Last. Sudhanshu Shukla(IITs: Indian Institutes of Technology)H-Index: 18
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Abstract null null Lung adenocarcinoma (LUAD) is a major health problem with minimal prognosis. Heterogeneity is a central determinant of the treatment outcome, requiring identification of new subclass. Senescence has emerged as a crucial mechanism regulating metastasis and drug response. We used IR- and Dox-induced senescence-associated genes in lung fibroblasts and performed K-means clustering to identify high (HS) and low senescence (LS) classes in TCGA-LUAD patients. The LS group patients sh...