Medicinal plants contain natural bio-active constituents in various parts called photochemical that are of therapeutic importance. This research was undertaken to determine the phytochemical constituents and in-vitro antioxidant activities of ethyl acetate extract of Dryopteris dilatata leaves (DDL). Phytochemical analysis was determined on the plant samples following established protocols. The antioxidant property of Dryopteris dilatata leaves was evaluated using ferric reducing antioxidant pow...
The prospects for using Moringa oleifera leaves extract (MLE) supplemented with different concentrations of kanwa or sodium bicarbonate (NaHCO3) as a low-cost alternative growth medium of Spirulina platensis were evaluated in a small-scale outdoor cultivation system. The present study was aimed to evaluate the potential of MLE growth medium enriched with different concentrations (4 or 8 g L-1) of kanwa and NaHCO3 on growth, chlorophyll content, biochemical characteristics, antioxidant compounds ...
Aerobic rice production system provides a sustainable alternative to the traditional rice cultivation. To evaluate the agronomic and economic effect of intercropping aerobic rice with four leafy vegetables, field experiments were conducted at University of Agriculture Science Bangalore research station, India during 2017 and 2018. The experiments consisted of 9 treatments with 4 replications and, a Randomized Completely Block Design was applied. The treatments were as follows: Intercropping (IC1...
Taro (Colocasia esculenta L. Schott) is an important crop for food and nutrition security, incomes, and livelihood of people in developing countries. Its cultivation and productivity have been limited mainly due to a lack of quality planting materials. Therefore, this study developed an effective micropropagation method for two common taro varieties in Kenya (Purple Wild and Dasheen) using apical meristem derived from portions of the corm and base of leaf petioles. This study optimized the 6-ben...
Due to its economic importance, wheat (Triticum aestivum L.) has been the subject of most breeding studies, most of which having as a starting point the in vitro cultures required for initiating different cell and tissues cultures such as protoplasts, ovules and pollen cells. All these procedures are used to obtain new varieties or hybrids regarding the improvement of different qualities such as higher productivity, more resistant towards pests and diseases, and to handle different environmental...
This work aimed at evaluating the behavioral, biochemical and hematological effects of pharmaceutical effluent in laboratory population of Clarias gariepinus using a static renewal bioassay system. Fish specimens were collected and exposed to five (0.04, 0.06, 0.08, 0.10 and 0.12 mgL-1) sublethal concentrations of the effluent including a control experiment. Different dose dependent behavioral responses such as erratic swimming, gasp for breath, restlessness, and constant upward movement were ob...
Ginseng plants associated with a number of fungi, several of which are reported to protect it from pathogens, thus improving plant growth. This study aimed to screen Panax ginseng leaves for endophytic fungi and to assess these fungi for their efficacy to inhibit ginseng black spot disease caused by Alternaria panax. A total of 256 endophytic fungal isolates were obtained from P. ginseng leaves. Most of the fungal isolates belonged to Chaetomium, Nemania, Xylaria, Nodulisporium and Alternaria; t...
Bacterial wilt caused by Ralstonia solanacearum is a major constraint to production of potatoes (Solanum tuberosum L). To date, there are no known conventional bactericides which provide effective control of this soil borne pathogen. This study aimed at investigating the antibacterial activity of eugenol and thymol which are the major components of thyme and clove essential oils, respectively against R. solanacearum. The pathogen was isolated from potato tubers exhibiting bacterial wilt symptoms...
Improved agricultural production of essential crops through advanced breeding is important for increasing access to nutritious food for the world's rapidly growing population, which is expected to reach 9.8 billion by 2050. Recent advancements in the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein9 (CRISPR/Cas9) genome editing process, which uses single-guide RNA for genome editing, have made it easy, stable and efficient tool for targeted gene mutations, knoc...
Acid phosphatase from mung bean (Vigna radiata) germinated seeds was partially purified via ammonium sulfate precipitation and fully purified via gel excision and heat denaturation, yielding a 29 kDa protein. Using 4-nitrophenylphosphate as a substrate, Vmax and Km values of 0.10 µmol/min and 0.27 mM, respectively, were obtained. The acid phosphatase was heat resistant, enhanced by the presence of Fe2+ and Mn2+ salts, and inhibited by the presence of citrate. Mung bean acid phosphatase reacted i...