Mariko Katoh-Kurasawa
Baylor College of Medicine
GenePhenotypeGenomeGATA transcription factorMutantCellular differentiationGene expressionTranscriptomeRegulation of gene expressionConserved sequenceAllorecognitionDictyosteliumDictyostelium discoideumDictyostelium purpureumTranscription (biology)MorphogenesisGATA2GeneticsRegulonRobustness (evolution)Gene regulatory networkComputational biologyMulticellular organismTranscription factorBiologyCell biologyProtein kinase A
16Publications
8H-index
433Citations
Publications 16
Newest
#1Mariko Katoh-Kurasawa (BCM: Baylor College of Medicine)H-Index: 8
#2Karin Hrovatin (University of Ljubljana)H-Index: 3
Last. Gad Shaulsky (BCM: Baylor College of Medicine)H-Index: 50
view all 0 authors...
Dictyostelium development begins with single-cell starvation and ends with multicellular fruiting bodies. Developmental morphogenesis is accompanied by sweeping transcriptional changes, encompassing nearly half of the 13,000 genes in the genome. We performed time-series RNA-sequencing analyses of the wild type and 20 mutants to explore the relationships between transcription and morphogenesis,. These strains exhibit developmental arrest at different stages, accelerated development, or atypical m...
1 CitationsSource
#1Shigenori Hirose (BCM: Baylor College of Medicine)H-Index: 6
#2Mariko Katoh-Kurasawa (BCM: Baylor College of Medicine)H-Index: 8
Last. Gad Shaulsky (BCM: Baylor College of Medicine)H-Index: 50
view all 0 authors...
Allorecognition and tissue formation are interconnected processes that require signaling between matching pairs of the polymorphic transmembrane proteins TgrB1 and TgrC1 in Dictyostelium. Extracellular and intracellular cAMP signaling are essential to many developmental processes. The three adenylate cyclase genes, acaA, acrA and acgA are required for aggregation, culmination, and spore dormancy, respectively, and some of their functions can be suppressed by activation of the cAMP-dependent prot...
Source
#1Mariko Katoh-Kurasawa (BCM: Baylor College of Medicine)H-Index: 8
#2Karin Hrovatin (University of Ljubljana)H-Index: 3
Last. Gad Shaulsky (BCM: Baylor College of Medicine)H-Index: 50
view all 7 authors...
Development of the social amoeba Dictyostelium discoideum begins by starvation of single cells and ends in multicellular fruiting bodies 24 hours later. These major morphological changes are accompanied by sweeping gene expression changes, encompassing nearly half of the 13,000 genes in the genome. To explore the relationships between the transcriptome and developmental morphogenesis, we performed time-series RNA-sequencing analysis of the wild type and 20 mutant strains with altered morphogenes...
Source
#1Peter Kundert (BCM: Baylor College of Medicine)H-Index: 3
#2Alejandro Sarrion-Perdigones (BCM: Baylor College of Medicine)H-Index: 11
Last. Gad Shaulsky (BCM: Baylor College of Medicine)H-Index: 50
view all 8 authors...
GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent...
1 CitationsSource
#1Michelle RubinH-Index: 1
#2Amber D. Miller (BCM: Baylor College of Medicine)H-Index: 4
Last. Gad ShaulskyH-Index: 50
view all 6 authors...
The eukaryotic amoeba Dictyostelium discoideum is commonly used to study sociality. The amoebae cooperate during development, exhibiting altruism, cheating, and kin-discrimination, but growth while preying on bacteria has been considered asocial. Here we show that Dictyostelium are cooperative predators. Using mutants that grow poorly on Gram-negative bacteria but grow well on Gram-positive bacteria, we show that growth depends on cell-density and on prey type. We also found synergy, by showing ...
1 CitationsSource
#1William S. Swatson (MU: University of Missouri)H-Index: 2
#2Mariko Katoh-Kurasawa (BCM: Baylor College of Medicine)H-Index: 8
Last. Stephen Alexander (MU: University of Missouri)H-Index: 21
view all 4 authors...
Botanicals are widely used as dietary supplements and for the prevention and treatment of disease. Despite a long history of use, there is generally little evidence supporting the efficacy and safety of these preparations. Curcumin has been used to treat a myriad of human diseases and is widely advertised and marketed for its ability to improve health, but there is no clear understanding how curcumin interacts with cells and affects cell physiology. D. discoideum is a simple eukaryotic lead syst...
8 CitationsSource
#1Mariko Katoh-Kurasawa (BCM: Baylor College of Medicine)H-Index: 8
#2Balaji Santhanam (BCM: Baylor College of Medicine)H-Index: 12
Last. Gad Shaulsky (BCM: Baylor College of Medicine)H-Index: 50
view all 3 authors...
ABSTRACT The GATA transcription factor GtaG is conserved in Dictyostelids and is essential for terminal differentiation in Dictyostelium discoideum , but its function is not well understood. Here, we show that gtaG is expressed in prestalk cells at the anterior region of fingers and in the extending stalk during culmination. The gtaG − phenotype is cell-autonomous in prestalk cells and non-cell-autonomous in prespore cells. Transcriptome analyses reveal that GtaG regulates prestalk gene expressi...
3 CitationsSource
#1Balaji Santhanam (BCM: Baylor College of Medicine)H-Index: 12
#2Huaqing Cai (Johns Hopkins University)H-Index: 16
Last. Mariko Katoh-Kurasawa (BCM: Baylor College of Medicine)H-Index: 8
view all 5 authors...
Development involves dynamic transcriptional changes. By serial ChIP- and RNA-sequencing, here, the authors show that GtaC, a GATA type transcription factor, exhibits temporally distinctive DNA binding and regulation of gene expression concordant with the development in the social amoeba Dictyostelium discoideum.
10 CitationsSource
#1Elizabeth A. Ostrowski (UH: University of Houston)H-Index: 10
#2Yufeng Shen (Columbia University)H-Index: 73
Last. David C. Queller (WashU: Washington University in St. Louis)H-Index: 71
view all 26 authors...
Summary Cooperative systems are susceptible to invasion by selfish individuals that profit from receiving the social benefits but fail to contribute. These so-called "cheaters" can have a fitness advantage in the laboratory, but it is unclear whether cheating provides an important selective advantage in nature. We used a population genomic approach to examine the history of genes involved in cheating behaviors in the social amoeba Dictyostelium discoideum , testing whether these genes experience...
28 CitationsSource
#1Rafael D. Rosengarten (BCM: Baylor College of Medicine)H-Index: 4
#2Balaji Santhanam (BCM: Baylor College of Medicine)H-Index: 12
Last. Gad Shaulsky (BCM: Baylor College of Medicine)H-Index: 50
view all 7 authors...
Development of the soil amoeba Dictyostelium discoideum is triggered by starvation. When placed on a solid substrate, the starving solitary amoebae cease growth, communicate via extracellular cAMP, aggregate by tens of thousands and develop into multicellular organisms. Early phases of the developmental program are often studied in cells starved in suspension while cAMP is provided exogenously. Previous studies revealed massive shifts in the transcriptome under both developmental conditions and ...
35 CitationsSource