Deolinda Santinha
University of Coimbra
HaCaTDownregulation and upregulationMass spectrometryChemistryOxidative stressSerineMoietyCeramideLipopolysaccharidePhospholipidPhosphatidylserinePhosphatidylinositolPhosphatidylethanolamineCardiolipinLactosylceramideLipid oxidationLipid metabolismBiochemistryOxidative phosphorylationCell biology
13Publications
7H-index
146Citations
Publications 14
Newest
#1Patrícia R. Pitrez (UC: University of Coimbra)H-Index: 5
#2Luís M.B.B. Estronca (UC: University of Coimbra)H-Index: 8
Last. Lino Ferreira (UC: University of Coimbra)H-Index: 43
view all 21 authors...
2 CitationsSource
#1Katarzyna Buczak (University of Basel)H-Index: 7
#2Joanna Kirkpatrick (NIH: National Institutes of Health)H-Index: 12
Last. Alessandro Ori (NIH: National Institutes of Health)H-Index: 27
view all 9 authors...
Bottom-up mass spectrometry–based proteomics relies on protein digestion and peptide purification. The application of such methods to broadly available clinical samples such as formalin-fixed and paraffin-embedded (FFPE) tissues requires reversal of chemical crosslinking and the removal of reagents that are incompatible with mass spectrometry. Here, we describe in detail a protocol that combines tissue disruption by ultrasonication, heat-induced antigen retrieval and two alternative methods for ...
2 CitationsSource
#1Deolinda Santinha (University of Aveiro)H-Index: 7
#2Anna Klopot (KI: Karolinska Institutet)H-Index: 1
Last. Luisa A. Helguero (University of Aveiro)H-Index: 26
view all 14 authors...
Abstract Liver X receptor (LXR) agonists have the potential to alleviate obesity related diseases, particularly atherosclerosis. However, LXRs are transcriptional regulators that induce de novo lipogenesis and lipid accumulation in hepatocytes which represents a serious adverse effect. In this work, we sought to characterize the LXR agonist GW3965 effects on fatty acid (FA) and phospholipid (PL) remodelling and the correlation with gene expression in order to better understand the underlying eff...
2 CitationsSource
#1Ana Rita D. Araújo (University of Aveiro)
#2Tânia MeloH-Index: 15
Last. Manuel A. S. SantosH-Index: 36
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: The occurrence of protein synthesis errors (mistranslation) above the typical mean mistranslation level of 10-4 is mostly deleterious to yeast, zebrafish and mammal cells. Previous yeast studies have shown that mistranslation affects fitness and deregulates genes related to lipid metabolism, but there is no experimental proof that such errors alter yeast lipid profiles. We engineered yeast strains to misincorporate serine at alanine and glycine sites on a global scale and evaluated the putativ...
Source
#1Daniela Couto (University of Aveiro)H-Index: 3
#2Deolinda Santinha (University of Aveiro)H-Index: 7
Last. M. Rosário M. Domingues (University of Aveiro)H-Index: 32
view all 9 authors...
Galactosylceramide (GalCer) and lactosylceramide (LacCer) are structural and signaling lipids, playing important roles in signal transduction and cell adhesion. They are especially abundant in the nervous system and in important components of the myelin sheath. Although neurodegenerative disorders are associated with increased oxidative stress and lipid oxidation, the connection between oxidative stress and glycosphingolipid modification has been scarcely addressed. In this study, we aimed to ch...
9 CitationsSource
#1Deolinda Santinha (University of Aveiro)H-Index: 7
#2Emanuel Ferreira-Fernandes (University of Aveiro)H-Index: 2
Last. M. Rosário M. Domingues (University of Aveiro)H-Index: 32
view all 8 authors...
RATIONALE Glycosphingolipids are important lipid molecules namely as constituents of the plasma membrane organized in lipid rafts, in signal transduction, and cell-cell communication. Although many human diseases are associated with oxidative stress and lipid oxidation, a link between oxidative stress and modification of glycosphingolipids has never been addressed. METHODS In this study, the structural changes caused by UVA-induced photooxidation of galactosyl- (GalCer) and lactosylceramide (Lac...
5 CitationsSource
#1R. Faria (University of Aveiro)H-Index: 1
#2Magda M. Santana (UC: University of Coimbra)H-Index: 9
Last. Maria Rosário Domingues (University of Aveiro)H-Index: 27
view all 12 authors...
Abstract Depression is a worldwide disability disease associated with high morbidity and has increased dramatically in the last few years. The differential diagnosis and the definition of an individualized therapy for depression are hampered by the absence of specific biomarkers. The aim of this study was to evaluate the phospholipidomic profile of the brain and myocardium in a mouse model of depression induced by chronic unpredictable stress (CUS). The lipidomic profile was evaluated by thin la...
33 CitationsSource
#1Diana Antunes (University of Aveiro)H-Index: 1
#2Ana Isabel Padrão (University of Aveiro)H-Index: 13
Last. Rita Ferreira (University of Aveiro)H-Index: 32
view all 15 authors...
Abstract Alterations in muscle mitochondrial bioenergetics during cancer cachexia were previously suggested; however, the underlying mechanisms are not known. So, the goal of this study was to evaluate mitochondrial phospholipid remodeling in cancer-related muscle wasting and its repercussions to respiratory chain activity and fiber susceptibility to apoptosis. An animal model of urothelial carcinoma induced by exposition to N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN) and characterized by signi...
40 CitationsSource
#1Elisabete Maciel (University of Aveiro)H-Index: 16
#2Bruno Miguel Neves (University of Aveiro)H-Index: 18
Last. M. Rosário M. Domingues (University of Aveiro)H-Index: 32
view all 9 authors...
Phosphatidylserine (PS) is preferentially located in the inner leaflet of the cell membrane, and translocation of PS oxidized in fatty acyl chains to the outside of membrane has been reported as signaling to macrophage receptors to clear apoptotic cells. It was recently shown that PS can be oxidized in serine moiety of polar head-group. In the present work, a targeted lipidomic approach was applied to detecting OxPS modified at the polar head-group in keratinocytes that were exposed to the radic...
16 CitationsSource
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