Gina Lisignoli
University of Bologna
ChemokineEndocrinologyPathologyMolecular biologyStromal cellAdipose tissueChemistryMesenchymal stem cellImmunologyOsteoarthritisOsteoblastCytokineBone marrowCartilageChondrogenesisCancer researchMedicineBiologyScaffoldCell biology
196Publications
37H-index
3,758Citations
Publications 191
Newest
#1Shikha ChawlaH-Index: 8
#2Giovanna DesandoH-Index: 15
Last. Sourabh GhoshH-Index: 23
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Major limitation of 3D bioprinting is the poor understanding of the role of bioink in modulating molecular signaling pathways. Phenotypically stable engineered articular cartilage was fabricated using silk fibroin–gelatin (SF-G) bioink and progenitor cells or mature articular chondrocytes. In the current study, role of SF-G bioink in modulating in vitro chondrogenic signaling pathways in human bone marrow-derived stromal cells (hMSCs) is elucidated. The interaction between SF-G bioink and hMSCs ...
1 CitationsSource
#1Luciana Sartore (University of Brescia)H-Index: 23
#2Cristina ManferdiniH-Index: 19
Last. Gina LisignoliH-Index: 37
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Abstract Selection of feasible hybrid-hydrogels for best chondrogenic differentiation of human mesenchymal stromal cells (hMSCs) represents an important challenge in cartilage regeneration. In this study, three-dimensional hybrid hydrogels obtained by chemical crosslinking of poly (ethylene glycol) diglycidyl ether (PEGDGE), gelatin (G) without or with chitosan (Ch) or dextran (Dx) polysaccharides were developed. The hydrogels, namely G-PEG, G-PEG-Ch and G-PEG-Dx, were prepared with an innovativ...
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#2Veronica IacovacciH-Index: 10
Last. Leonardo RicottiH-Index: 24
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This paper describes the design of a chip modeling the proximal renal tubule, namely the nephron segment that is most targeted by drug toxicants. The tubule model is based on a 3D convoluted geometry whose printing parameters and Pluronic concentration were optimized to achieve a diameter of 320~\mu \text{m}without discontinuities. A perfusion system guaranteeing a 1~\mu \text{L}/min flow was also integrated. Moreover, the tubule model was integrated into a gel recapitulating the compositi...
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#1Diego TruccoH-Index: 3
#2Lorenzo VannozziH-Index: 7
Last. Leonardo RicottiH-Index: 24
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#1Diego Trucco (Sant'Anna School of Advanced Studies)H-Index: 3
#2Lorenzo Vannozzi (Sant'Anna School of Advanced Studies)H-Index: 7
Last. Leonardo Ricotti (Sant'Anna School of Advanced Studies)H-Index: 24
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Articular cartilage (AC) is a specialized connective tissue able to provide a low-friction gliding surface supporting shock-absorption, reducing stresses, and guaranteeing wear-resistance thanks to its structure and mechanical and lubrication properties. Being an avascular tissue, AC has a limited ability to heal defects. Nowadays, conventional strategies show several limitations, which results in ineffective restoration of chondral defects. Several tissue engineering approaches have been propos...
1 CitationsSource
#1Saverio AffatatoH-Index: 32
#2Diego TruccoH-Index: 3
Last. Gina LisignoliH-Index: 37
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This paper aims to characterize the wear behavior of hydrogel constructs designed for human articular cartilage replacement. To this purpose, poly (ethylene glycol) diacrylate (PEGDA) 10% w/v and gellan gum (GG) 1.5% w/v were used to reproduce the superior (SUP) cartilage layer and PEGDA 15% w/v and GG 1.5% w/v were used to reproduce the deep (DEEP) cartilage layer, with or without graphene oxide (GO). These materials (SUP and DEEP) were analyzed alone and in combination to mimic the zonal archi...
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#1Andrea BianchettiH-Index: 6
#2Clizia Chinello (University of Milano-Bicocca)H-Index: 19
Last. Camillo AlmiciH-Index: 23
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Human Platelet Lysate (hPL) is considered a valid substitute to foetal bovine serum (FBS) in mesenchymal stromal cells (MSC) expansion and it is commonly produced starting from intermediate side-products of whole blood donations. Through freeze-thaw cycles, hPL is highly enriched in chemokines, growth factors and adhesion and immunologic molecules. Cell therapy protocols, using hPL instead of FBS for cells’ expansion, are approved by regulatory authorities without concerns and administration in ...
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#1Cristina ManferdiniH-Index: 19
#2Yasmin SalehH-Index: 1
Last. Gina LisignoliH-Index: 37
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There is a lack ofin vitromodels able to properly represent osteoarthritis (OA) synovial tissue (ST). We aimed to characterize OA ST and to investigate whether a mechanical or enzymatic digestion procedures influence synovial cell functional heterogeneity in vitro. Procedures using mechanical nondigested fragments (NDF), synovial digested fragments (SDF), and filtrated synovial digested cells (SDC) were compared. An immunophenotypic profile was performed to distinguish synovial fibroblasts (CD55...
2 CitationsSource
#1Federica Costa (University of Parma)H-Index: 11
#2Rosanna Vescovini (University of Parma)H-Index: 23
Last. Nicola Giuliani (University of Parma)H-Index: 58
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#1Elisa AssirelliH-Index: 9
#2Lia PulsatelliH-Index: 27
Last. Riccardo MeliconiH-Index: 26
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Objective To investigate complement(C) factors(F) and their activation fragments expression in OA joint tissues. Design Immunohistochemistry and quantitative imaging were performed to analyze C3, C4, and CF (factor) B expression on osteochondral biopsies (43 patients) collected during arthroplasty. Isolated chondrocytes and synoviocytes, cartilage and synovial tissues obtained from surgical specimens of OA patients (15 patients) were cultured with or without IL-1β. Real time PCR for CFB, C3, and...
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