Wim Jiskoot
Leiden University
Analytical chemistryBiophysicsProtein aggregationAntigenMolecular biologyNanoparticleVirologyAntibodyLiposomeChemistryMaterials scienceImmunologyVaccinationAdjuvantImmunogenicityChromatographyMonoclonal antibodyBiochemistryMedicineBiologyImmune systemMicrobiologyPharmacology
Publications 330
#1Shravan Sreenivasan (IITD: Indian Institute of Technology Delhi)H-Index: 2
#2Wim Jiskoot (LEI: Leiden University)H-Index: 85
Last. Anurag S. Rathore (IITD: Indian Institute of Technology Delhi)H-Index: 34
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Abstract null null Degradation of therapeutic monoclonal antibodies (mAb) due to interfacial agitation through air bubbling was investigated. Samples containing mAb in phosphate buffered saline were subjected to rapid bubbling by using a peristaltic pump at an air flow rate of 11.5 mL/min. Samples were analyzed by visual observation, UV–Vis, fluorescence, circular dichroism and infrared spectroscopy, size-exclusion chromatography (SEC), dynamic light scattering, microscopy, and cell-based activi...
#1Tapan K. Das (BMS: Bristol-Myers Squibb)H-Index: 27
#2Alavattam Sreedhara (Genentech)H-Index: 27
Last. Wim Jiskoot (LEI: Leiden University)H-Index: 85
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Abstract null null Injectable protein-based medicinal products (drug products, or DPs) must be produced by using sterile manufacturing processes to ensure product safety. In DP manufacturing the protein drug substance, in a suitable final formulation, is combined with the desired primary packaging (e.g., syringe, cartridge, or vial) that guarantees product integrity and enables transportation, storage, handling and clinical administration. The protein DP is exposed to several stress conditions d...
#1Bart Boersma (University of Geneva)
#2Wim Jiskoot (LEI: Leiden University)H-Index: 85
Last. Carole Bourquin (University of Geneva)H-Index: 31
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The interleukin-1 (IL-1) family is one of the first described cytokine families and consists of eight cytokines (IL-1β, IL-1α, IL-18, IL-33, IL-36α, IL-36β, IL-36γ and IL-37) and three receptor antagonists (IL-1Ra, IL-36Ra and IL-38). The family members are known to play an essential role in inflammation. The importance of inflammation in cancer has been well established in the past decades. This review sets out to give an overview of the role of each IL-1 family member in cancer pathogenesis an...
#1Adam Grabarek (LEI: Leiden University)H-Index: 3
#2Wim Jiskoot (LEI: Leiden University)H-Index: 85
Last. T. Menzen (LEI: Leiden University)
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Abstract null null Cell-based medicinal products (CBMPs) offer ground-breaking opportunities to treat diseases with limited or no therapeutic options. However, the intrinsic complexity of CBMPs results in great challenges with respect to analytical characterization and stability assessment. In our study, we submitted Jurkat cell suspensions to forced degradation studies mimicking conditions to which CBMPs might be exposed from procurement of cells to administration of the product. Flow imaging m...
#1Linde Schoenmaker (LEI: Leiden University)H-Index: 1
#2Dominik Witzigmann (UBC: University of British Columbia)H-Index: 20
Last. Daan J.A. Crommelin (UU: Utrecht University)H-Index: 90
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Abstract A drawback of the current mRNA-lipid nanoparticle (LNP) COVID-19 vaccines is that they have to be stored at (ultra)low temperatures. Understanding the root cause of the instability of these vaccines may help to rationally improve mRNA-LNP product stability and thereby ease the temperature conditions for storage. In this review we discuss proposed structures of mRNA-LNPs, factors that impact mRNA-LNP stability and strategies to optimize mRNA-LNP product stability. Analysis of mRNA-LNP st...
#1Thomas J. M. Michiels (LEI: Leiden University)H-Index: 4
#2Madelief A van Veen (LEI: Leiden University)
Last. Bernard MetzH-Index: 17
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Currently, animal tests are being used to confirm the potency and lack of toxicity of toxoid vaccines. In a consistency approach, animal tests could be replaced if production consistency (compared to known good products) can be proven in a panel of in vitro assays. By mimicking the in vivo antigen processing in a simplified in vitro approach, it may be possible to distinguish aberrant products from good products. To demonstrate this, heat-exposed diphtheria toxoid was subjected to partial digest...
#1Jihui Lee (LEI: Leiden University)H-Index: 1
#2Koen van der Maaden (LUMC: Leiden University Medical Center)H-Index: 15
Dissolving microneedle arrays (dMNAs) are promising devices for intradermal vaccine delivery. The aim of this study was to develop a reproducible fabrication method for dMNAs based on an automated nano-droplet dispensing system that minimizes antigen waste. First, a polymer formulation was selected to dispense sufficiently small droplets (<18 nL) that can enter the microneedle cavities (base diameter 330 µm). Besides, three linear stages were assembled to align the dispenser with the cavities, a...
#1Karin H. Hoogendoorn (LUMC: Leiden University Medical Center)H-Index: 6
#2Daan J.A. Crommelin (UU: Utrecht University)H-Index: 90
Last. Wim Jiskoot (LEI: Leiden University)H-Index: 85
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Abstract The formulation of cell-based medicinal products (CBMPs) poses major challenges because of their complexity, heterogeneity, interaction with their environment (e.g., the formulation buffer, interfaces), and susceptibility to degradation. These challenges can be quality, safety, and efficacy related. In this commentary we discuss the current status in formulation strategies of off-the-shelf and non-off-the-shelf (patient-specific) CBMPs and highlight advantages and disadvantages of each ...
#1Jeroen HeutsH-Index: 2
#2Wim JiskootH-Index: 85
Last. Koen van der MaadenH-Index: 15
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Cationic nanoparticles have been shown to be surprisingly effective as cancer vaccine vehicles in preclinical and clinical studies. Cationic nanoparticles deliver tumor-associated antigens to dendritic cells and induce immune activation, resulting in strong antigen-specific cellular immune responses, as shown for a wide variety of vaccine candidates. In this review, we discuss the relation between the cationic nature of nanoparticles and the efficacy of cancer immunotherapy. Multiple types of li...
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