Maryam Ghayeb Zamharir
Host (biology)Nested polymerase chain reactionVirologyRestriction fragment length polymorphismPhylogenetic treeInducerBroomPhytoplasmaTaqIDiseaseRRNA OperonSample collection16S ribosomal RNAVeterinary medicineGeneticsResistance (ecology)Phytosanitary certificationBotanySequence analysisRibosomal RNABiology
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#2Samanta Paltrinieri (UNIBO: University of Bologna)H-Index: 15
Last. Assunta Bertaccini (UNIBO: University of Bologna)H-Index: 6
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Grapevine (Vitis vinifera) is one of the most important fruits in Iran where the provinces of Qazvin, Lorestan and Markazi are main producers. During 2013–2015, vineyards located in these provinces were surveyed to verify the presence of phytoplasma. The sample collection was based on symptomatology including decline, leaf yellowing and shortening of internodes. Total DNA was extracted from symptomatic and symptomless grapevine samples and used in nested-polymerase chain reaction (PCR) assays wi...
16 CitationsSource
Pistachio is an important crop in Iran, which is a major producer and exporter of pistachio nuts. The occurrence of a new disease of pistachio trees, characterized by the development of severe witches' broom, stunted growth and leaf rosetting, was observed in Ghazvin Province. A phytoplasma was detected in infected trees by polymerase chain reaction (PCR) amplification of rRNA operon sequences. Nested PCR with primer pairs P1/P7 and R16F2n/R16R2 was used for specific detection of the phytoplasma...
7 CitationsSource