Yuanfei Zhu
Fudan University
EndosomeProtein subunitHost (biology)VirologyChemistryIn vitroVirusCytopathic effectChemotherapyCoronavirusPorphyrinProtoporphyrin IXVerteporfinViral ReceptorSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Tissue tropismGeneticsViral entryMedicineBiologyCell biologyEC50
14Publications
8H-index
188Citations
Publications 11
Newest
#5Shuai XiaH-Index: 18
#6Chenjian GuH-Index: 10
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#4Chenjian Gu (Fudan University)H-Index: 10
Massive production of efficacious SARS-CoV-2 vaccines is essential for controlling the ongoing COVID-19 pandemic. We report here the preclinical development of yeast-produced receptor-binding domain (RBD)-based recombinant protein SARS-CoV-2 vaccines. We found that monomeric RBD of SARS-CoV-2 could be efficiently produced as a secreted protein from transformed Pichia pastoris (P. pastoris) yeast. Yeast-derived RBD-monomer possessed functional conformation and was able to elicit protective level ...
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The SARS-CoV-2 infection is spreading rapidly worldwide. Efficacious antiviral therapeutics against SARS-CoV-2 is urgently needed. Here, we discovered that protoporphyrin IX (PpIX) and verteporfin, two FDA-approved drugs, completely inhibited the cytopathic effect produced by SARS-CoV-2 infection at 1.25 µmol/Land 0.31 µmol/L respectively, and their EC50 values of reduction of viral RNA were at nanomolar concentrations. The selectivity indices of PpIX and verteporfin were 952.74 and 368.93, resp...
12 CitationsSource
#1Yinghui Liu (Center for Infectious Disease Research and Policy)H-Index: 1
#2Gaowei Hu (Fudan University)H-Index: 5
Last. Qiang Ding (THU: Tsinghua University)H-Index: 64
view all 25 authors...
The pandemic of COVID-19, caused by SARS-CoV-2, is a major global health threat. Epidemiological studies suggest that bats (Rhinolophus affinis) are the natural zoonotic reservoir for SARS-CoV-2. However, the host range of SARS-CoV-2 and intermediate hosts that facilitate its transmission to humans remain unknown. The interaction of coronavirus with its host receptor is a key genetic determinant of host range and cross-species transmission. SARS-CoV-2 uses angiotensin-converting enzyme 2 (ACE2) ...
39 CitationsSource
#1Yunkai Zhu (Fudan University)H-Index: 4
#2Fei Feng (Fudan University)H-Index: 3
Last. Rong Zhang (Fudan University)H-Index: 10
view all 19 authors...
The global spread of SARS-CoV-2 is posing major public health challenges. One feature of SARS-CoV-2 spike protein is the insertion of multi-basic residues at the S1/S2 subunit cleavage site. Here, we find that the virus with intact spike (Sfull) preferentially enters cells via fusion at the plasma membrane, whereas a clone (Sdel) with deletion disrupting the multi-basic S1/S2 site utilizes an endosomal entry pathway. Using Sdel as model, we perform a genome-wide CRISPR screen and identify severa...
50 CitationsSource
#1Chao Zhang (CAS: Chinese Academy of Sciences)H-Index: 28
#2Yifan Wang (CAS: Chinese Academy of Sciences)H-Index: 5
Last. Zhong Huang (CAS: Chinese Academy of Sciences)H-Index: 29
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The ongoing pandemic of coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Neutralizing antibodies against SARS-CoV-2 are an option for drug development for treating COVID-19. Here, we report the identification and characterization of two groups of mouse neutralizing monoclonal antibodies (MAbs) targeting the receptor-binding domain (RBD) on the SARS-CoV-2 spike (S) protein. MAbs 2H2 and 3C1, representing the two antibody groups, respec...
21 CitationsSource
#1Yunkai Zhu (Fudan University Shanghai Medical College)
#2Fei Feng (Fudan University Shanghai Medical College)
Last. Zhenghong Yuan (Fudan University Shanghai Medical College)H-Index: 45
view all 18 authors...
The global spread of SARS-CoV-2 is posing major public health challenges. One unique feature of SARS-CoV-2 spike protein is the insertion of multi-basic residues at the S1/S2 subunit cleavage site, the function of which remains uncertain. We found that the virus with intact spike (Sfull) preferentially enters cells via fusion at the plasma membrane, whereas a clone (Sdel) with deletion disrupting the multi-basic S1/S2 site instead utilizes a less efficient endosomal entry pathway. This idea was ...
29 CitationsSource
#1Chenjian Gu (Fudan University Shanghai Medical College)
Abstract The SARS-CoV-2 infection is spreading rapidly worldwide. Efficacious antiviral therapeutics against SARS-CoV-2 is urgently needed. Here, we discovered that protoporphyrin IX (PpIX) and verteporfin, two FDA-approved drugs, completely inhibited the cytopathic effect produced by SARS-CoV-2 infection at 1.25 μM and 0.31 μM respectively, and their EC50 values of reduction of viral RNA were at nanomolar concentrations. The selectivity indices of PpIX and verteporfin were 952.74 and 368.93, re...
6 CitationsSource
#1Chenjian Gu (Fudan University Shanghai Medical College)
The infection of SARS-CoV-2 has spread to more than 200 countries and regions and the numbers of infected people and deaths worldwide are expected to continue to rise. Current treatment of COVID-19 is limited and mostly supportive. At present, there is no specific therapeutics against SARS-CoV-2. In this study, we discovered that protoporphyrin IX and verteporfin, two FDA-approved drugs for treatment of human diseases, had significant antiviral effect against SARS-CoV-2, with EC50 values for the...
10 CitationsSource
#1Yinghui Liu (THU: Tsinghua University)H-Index: 1
#2Gaowei Hu (Fudan University)H-Index: 5
Last. Qiang Ding (THU: Tsinghua University)H-Index: 64
view all 19 authors...
The pandemic of a newly emerging coronavirus (SARS-CoV-2), the causative agent of severe pneumonia disease (COVID-19), is threatening global health. Epidemiological studies suggested that bats are the natural reservoir hosts for SARS-CoV-2, however, the host range of SARS-CoV-2 and the identity of intermediate hosts facilitating the transmission to humans remains unknown. Coronavirus-receptor interaction is a key genetic determinant of the host range, cross-species transmission, and tissue tropi...
49 CitationsSource