Godwin Sokpor
Ruhr University Bochum
Protein–protein interactionFunction (biology)Embryonic stem cellCellGene expressionCell fate determinationRegulation of gene expressionChromatin remodelingNeuroscienceChemistryDNA methylationProgenitor cellN6-MethyladenosineCell sortingPeptideMRNA methylationProtein BiochemistryCell specificCortical tissueEpigeneticsImmunostainingProteomicsHigh-throughput screeningRNAComputational biologyBiologyCell biologyCRISPR
3Publications
1H-index
2Citations
Publications 3
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#1Godwin Sokpor (RUB: Ruhr University Bochum)H-Index: 1
#2Yuanbin Xie (HMU: Hainan Medical University)
Last. Tran Tuoc (RUB: Ruhr University Bochum)H-Index: 16
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Dynamic modification of RNA affords proximal regulation of gene expression triggered by non-genomic or environmental changes. One such epitranscriptomic alteration in RNA metabolism is the installation of a methyl group on adenosine (m6A) known to be the most prevalent modified state of mRNA in the mammalian cell. The methylation machinery responsible for the dynamic deposition and recognition of m6A on mRNA is composed of subunits that play specific roles, including reading, writing and erasing...
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#2Godwin Sokpor (RUB: Ruhr University Bochum)H-Index: 1
Last. Tran Tuoc (RUB: Ruhr University Bochum)H-Index: 16
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Summary Identification of peptides mediating protein-protein interaction (PPI) is crucial for understanding the function of interlinked proteins in cellular processes and amino acid-associated diseases. Traditional PPI assays are laborious, involving the generation of many truncated proteins. SPOT peptide assay allows high-throughput detection of domains essential for PPI by synthesizing several hundred peptides on a cellulose membrane. Here, we present a rapid SPOT peptide protocol for identify...
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#1M. Sadman Sakib (German Center for Neurodegenerative Diseases)H-Index: 6
#2Godwin Sokpor (RUB: Ruhr University Bochum)H-Index: 1
Last. Tran Tuoc (RUB: Ruhr University Bochum)H-Index: 16
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Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types. FACS has many challenges that limit comprehensive cell purification from the brain, leading to incomplete molecular characterization. Here, we present the intranuclear immunostaining-based FACS protocol with several modified steps, which allows optimized ...
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