Daniel F. Earley
University of Zurich
PharmacokineticsSurface modificationFlow chemistryAntibodyNanotechnologyCovalent bondBiochemical engineeringChemistryConjugateChelationIn vitroIn vivoCombinatorial chemistryBioconjugationRadiosynthesisHuman serum albuminPalette (painting)Context (language use)Cover (algebra)LinkerScope (project management)AzideArylFlow processLight activatedWater solubleMoleculeFlow (mathematics)Monoclonal antibodyPEG ratioYield (chemistry)BiochemistryFeature (computer vision)PhotochemistrySelection (genetic algorithm)MicrofluidicsFluorescence
8Publications
2H-index
6Citations
Publications 8
Newest
#1Amaury Guillou (UZH: University of Zurich)H-Index: 2
#2Daniel F. Earley (UZH: University of Zurich)H-Index: 2
Last. Jason P. Holland (UZH: University of Zurich)H-Index: 30
view all 7 authors...
Most experimental work in the space of bioconjugation chemistry focuses on using new methods to construct covalent bonds between a cargo molecule and a protein of interest such as a monoclonal antibody (mAb). Bond formation is important for generating new diagnostic tools, yet when these compounds advance to preclinical in vitro and in vivo studies, and later for translation to the clinic, understanding the fate of potential metabolites that arise from chemical or enzymatic degradation of the co...
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#1Daniel F. EarleyH-Index: 2
#2Amaury GuillouH-Index: 2
Last. Jason P. HollandH-Index: 30
view all 5 authors...
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#1Daniel F. Earley (UZH: University of Zurich)H-Index: 2
#2Amaury Guillou (UZH: University of Zurich)H-Index: 2
Last. Jason P. Holland (UZH: University of Zurich)H-Index: 30
view all 5 authors...
89Zr-radiolabelled proteins functionalised with desferrioxamine B are a cornerstone of diagnostic positron emission tomography. In the clinical setting, 89Zr-labelled proteins are produced manually. Here, we explore the potential of using a microfluidic photochemical flow reactor to prepare 89Zr-radiolabelled proteins. The light-induced functionalisation and 89Zr-radiolabelling of human serum albumin ([89Zr]ZrDFO-PEG3-Et-azepin-HSA) was achieved by flow photochemistry with a decay-corrected radi...
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#1Daniel F. EarleyH-Index: 2
#2Amaury GuillouH-Index: 2
Last. Jason P. HollandH-Index: 30
view all 5 authors...
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#1Jennifer Lamb (UZH: University of Zurich)H-Index: 2
#2Faustine d'Orchymont (UZH: University of Zurich)H-Index: 1
Last. Jason P. Holland (UZH: University of Zurich)H-Index: 30
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The synthesis, characterisation and application of radiolabelled compounds for use in diagnostic and therapeutic medicine requires a diverse skill set. This article highlights a selection of our ongoing projects that aim to provide new synthetic methods and radiochemical tools for building molecular imaging agents with various radionuclides.
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#1Amaury Guillou (UZH: University of Zurich)H-Index: 2
#2Daniel F. Earley (UZH: University of Zurich)H-Index: 2
Last. Jason P. Holland (UZH: University of Zurich)H-Index: 30
view all 4 authors...
Efficient methods to functionalize proteins are essential for the development of many diagnostic and therapeutic compounds, such as fluorescent probes for immunohistochemistry, zirconium-89 radiolabeled mAbs (89Zr-mAbs) for positron emission tomography and antibody-drug conjugates (ADCs). This protocol describes a step-by-step procedure for the light-induced functionalization of proteins with compounds bearing the photochemically active aryl azide group. As an illustration of the potential utili...
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#1Amaury Guillou (UZH: University of Zurich)H-Index: 2
#2Daniel F. Earley (UZH: University of Zurich)H-Index: 2
Last. Jason P. Holland (UZH: University of Zurich)H-Index: 30
view all 3 authors...
Protein-conjugates are vital tools in biomedical research, drug discovery and imaging science. For example, functionalised monoclonal antibodies (mAbs) coupled to the desferrioxamine B (DFO) chelate and radiolabelled with 89Zr are used as radiopharmaceuticals for diagnostic positron emission tomography (PET). In this context, protein functionalisation requires the formation of a covalent bond which must be achieved without compromising the biological properties of the mAb. Photochemistry offers ...
2 CitationsSource
#1Amaury Guillou (UZH: University of Zurich)H-Index: 2
#2Daniel F. Earley (UZH: University of Zurich)H-Index: 2
Last. Jason P. Holland (UZH: University of Zurich)H-Index: 30
view all 3 authors...
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