Ayman Abouzayed
Uppsala University
Denaturation (biochemistry)Ankyrin repeatCancerBiophysicsDownregulation and upregulationCircular dichroismMolecular imagingInternalizationEffective dose (radiation)ReceptorChemistryConjugateChelationIn vitroIn vivoBinding domainPositron emission tomographyIn silicoAlbuminTyrosineProstateEpithelial cell adhesion moleculeGastrin-releasing peptide receptorAntagonistDARPinPhenylalanineIC50BiodistributionEmission computed tomographyBombesinHuman serum albuminCell sortingPeptideLNCaPPeptide synthesisDOTATriple-negative breast cancerProstate cancerPancreatic cancerSingle-photon emission computed tomographyLinkerAdenocarcinomaTargeted therapyCancer therapyBlood circulationNuclear medicineRadionuclide therapyNegative chargePet imagingComparative evaluationDelayed imagingTumor targetingTumour targetingMurine modelMoleculeTechnetium-99mCancer researchAmino acidPiBreast cancerScaffold proteinPEG ratioDomain (ring theory)Signal transductionText miningMedicineDosimetryMechanism (biology)Directed evolutionCell biologyCancer cellBinding selectivityPharmacology
14Publications
4H-index
34Citations
Publications 15
Newest
#1Sara S. RinneH-Index: 8
Last. Anna Orlova (Uppsala University)H-Index: 53
view all 8 authors...
HER3 (human epidermal growth factor receptor type 3) is a challenging target for diagnostic radionuclide molecular imaging due to the relatively modest overexpression in tumors and substantial expression in healthy organs. In this study, we compared four HER3-targeting PET tracers based on different types of targeting molecules in a preclinical model: the 89Zr-labeled therapeutic antibody seribantumab, a seribantumab-derived F(ab)2-fragment labeled with 89Zr and 68Ga, and the 68Ga-labeled affibo...
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#1Sara S. Rinne (Uppsala University)H-Index: 8
#2Ayman Abouzayed (Uppsala University)H-Index: 4
Last. Anna Orlova (Tomsk Polytechnic University)H-Index: 53
view all 5 authors...
Molecular imaging of the gastrin-releasing peptide receptor (GRPR) could improve patient management in prostate cancer. This study aimed to produce gallium-66 (T½ = 9.5 h) suitable for radiolabeling, and investigate the imaging properties of gallium-66 labeled GRPR-antagonist NOTA-PEG2-RM26 for later-time point PET-imaging of GRPR expression. Gallium-66 was cyclotron-produced using a liquid target, and enriched [66Zn]Zn(NO3)2. In vitro, [66Ga]Ga-NOTA-PEG2-RM26 was characterized in GRPR-expressin...
2 CitationsSource
#1Ayman Abouzayed (Uppsala University)H-Index: 4
#2Sara S. Rinne (Uppsala University)H-Index: 8
Last. Anna Orlova (Uppsala University)H-Index: 53
view all 7 authors...
Background: Gastrin-releasing peptide receptor (GRPR) is an important target for imaging of prostate cancer. The wide availability of single-photon emission computed tomography/computed tomography (SPECT/CT) and the generator-produced 99mTc can be utilized to facilitate the use of GRPR-targeting radiotracers for diagnostics of prostate cancers. Methods: Synthetically produced mercaptoacetyl-Ser-Ser-Ser (maSSS)-PEG2-RM26 and mercaptoacetyl-Ser-Glu-Ser (maSES)-PEG2-RM26 (RM26 = d-Phe-Gln-Trp-Ala-V...
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Increasing thermal stability and improving biodistribution of VEGFR2-binding affibody molecules by a combination of in silico and directed evolution approaches
1 CitationsSource
#1Ayman AbouzayedH-Index: 4
#2Hanna TanoH-Index: 1
Last. Anna OrlovaH-Index: 53
view all 10 authors...
The targeting of gastrin-releasing peptide receptors (GRPR) was recently proposed for targeted therapy, e.g., radiotherapy. Multiple and frequent injections of peptide-based therapeutic agents would be required due to rapid blood clearance. By conjugation of the GRPR antagonist RM26 (D-Phe-Gln-Trp-Ala-Val-Gly-His-Sta-Leu-NH2) to an ABD (albumin-binding domain), we aimed to extend the blood circulation of peptides. The synthesized conjugate DOTA-ABD-RM26 was labelled with indium-111 and evaluated...
1 CitationsSource
#1Anzhelika Vorobyeva (Uppsala University)H-Index: 11
#2Ekaterina Bezverkhniaia (Tomsk Polytechnic University)H-Index: 1
Last. Vladimir Tolmachev (Uppsala University)H-Index: 61
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Efficient treatment of disseminated triple-negative breast cancer (TNBC) remains an unmet clinical need. The epithelial cell adhesion molecule (EpCAM) is often overexpressed on the surface of TNBC cells, which makes EpCAM a potential therapeutic target. Radionuclide molecular imaging of EpCAM expression might permit selection of patients for EpCAM-targeting therapies. In this study, we evaluated a scaffold protein, designed ankyrin repeat protein (DARPin) Ec1, for imaging of EpCAM in TNBC. DARPi...
4 CitationsSource
#1Fanny Lundmark (Uppsala University)H-Index: 1
#2Ayman Abouzayed (Uppsala University)H-Index: 4
Last. Anna Orlova (Uppsala University)H-Index: 53
view all 9 authors...
Prostate-specific membrane antigen (PSMA) and gastrin-releasing peptide receptor (GRPR) are promising targets for molecular imaging of prostate cancer (PCa) lesions. Due to the heterogenic overexpression of PSMA and GRPR in PCa, a heterodimeric radiotracer with the ability to bind to both targets could be beneficial. Recently, our group reported the novel heterodimer BQ7800 consisting of a urea-based PSMA inhibitor, the peptide-based GRPR antagonist RM26 and NOTA chelator. The study reported her...
2 CitationsSource
#1Sergey M. Deyev (Tomsk Polytechnic University)H-Index: 29
#2Anzhelika Vorobyeva (Tomsk Polytechnic University)H-Index: 11
Last. Vladimir Tolmachev (Tomsk Polytechnic University)H-Index: 61
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Abstract Radionuclide-based imaging of molecular therapeutic targets might facilitate stratifying patients for specific biotherapeutics. New type of imaging probes, based on designed ankyrin repeat proteins (DARPins), have demonstrated excellent contrast of imaging of human epidermal growth factor type 2 (HER2) expression in preclinical models. We hypothesized that labeling approaches, which result in lipophilic radiometabolites (non-residualizing labels), would provide the best imaging contrast...
9 CitationsSource
Conjugation of GRPR-targeting antagonist RM26 to albumin-binding domain extends antagonist's blood circulation and residence in tumours
#1Sara S. Rinne (Uppsala University)H-Index: 8
#2Charles Dahlsson Leitao (KTH: Royal Institute of Technology)H-Index: 6
Last. Anna Orlova (Uppsala University)H-Index: 53
view all 9 authors...
Upregulation of the human epidermal growth factor receptor type 3 (HER3) is a common mechanism to bypass HER-targeted cancer therapy. Affibody-based molecular imaging has the potential for detecting and monitoring HER3 expression during treatment. In this study, we compared the imaging properties of newly generated 68Ga-labeled anti-HER3 affibody molecules (HE)3-ZHER3-DOTA and (HE)3-ZHER3-DOTAGA with previously reported [68Ga]Ga-(HE)3-ZHER3-NODAGA. We hypothesized that increasing the negative ch...
10 CitationsSource