Milena Simovic
German Cancer Research Center
Homologous recombinationInduced pluripotent stem cellCancerDNA repairGenomeDNA damageCell typeApoptosisEmbryoOocyteGameteBlastocystLarge cellDNA Damage RepairIn Vitro Oocyte Maturation TechniquesGerminal vesicleReproductive technologyRepair processesMycn amplificationIn vitro toxicologyCancer researchDNAChromatinBiologyCell biologyChromothripsis
6Publications
2H-index
36Citations
Publications 5
Newest
#1Parra Rg (DKFZ: German Cancer Research Center)
#2Przybilla Mj (Wellcome Trust Sanger Institute)
Last. Thorsten Kolb (DKFZ: German Cancer Research Center)H-Index: 10
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Chromothripsis is a form of genome instability, whereby a presumably single catastrophic event generates extensive genomic rearrangements of one or few chromosome(s). However, little is known about the heterogeneity of chromothripsis across different clones from the same tumor, as well as changes in response to treatment. We analyzed single-cell genomic and transcriptomic alterations linked with chromothripsis in human p53-deficient medulloblastoma (n=7). We reconstructed the order of somatic ev...
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#1Thorsten Kolb (DKFZ: German Cancer Research Center)H-Index: 10
#2Umar Khalid (DKFZ: German Cancer Research Center)H-Index: 1
Last. Aurélie Ernst (DKFZ: German Cancer Research Center)H-Index: 16
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In vitro assays for clustered DNA lesions will facilitate the analysis of the mechanisms underlying complex genome rearrangements such as chromothripsis, including the recruitment of repair factors to sites of DNA double-strand breaks. We present a novel method generating localized DNA double-strand breaks using UV-irradiation with photomasks. The size of the damage foci and the spacing between lesions are fully adjustable, making the assay suitable for different cell types and targeted areas. W...
1 CitationsSource
#1Manasi RatnaparkheH-Index: 2
#2John K.L. WongH-Index: 9
Last. Aurélie ErnstH-Index: 16
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Chromothripsis and chromoanasynthesis are catastrophic events leading to clustered genomic rearrangements. Whole-genome sequencing revealed frequent complex genomic rearrangements (n= 16/26) in brain tumors developing in mice deficient for factors involved in homologous recombination repair or non-homologous end-joining. Catastrophic events were tightly linked to Myc/Mycn amplification, with increased DNA damage and inefficient apoptotic response already observable at early postnatal stages. Inh...
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#1Federica Cavalera (UNIPV: University of Pavia)H-Index: 2
#2Milena Simovic (DKFZ: German Cancer Research Center)H-Index: 2
Last. Maurizio Zuccotti (UNIPV: University of Pavia)H-Index: 31
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In the ovary, acquisition of oocyte developmental competence depends on a bidirectional exchange between the gamete and its companion cumulus cells (CCs). In this study we investigated the contribution of CCs surrounding oocytes of known developmental competence or incompetence to the acquisition of oocyte developmental competence. To this end, feeder layers of CCs (FL-CCs) were prepared using CCs isolated either from: (1) developmentally competent mouse oocytes whose nucleolus was surrounded by...
1 CitationsSource
#1Manasi Ratnaparkhe (DKFZ: German Cancer Research Center)H-Index: 2
#2John K.L. Wong (DKFZ: German Cancer Research Center)H-Index: 9
Last. Aurélie Ernst (DKFZ: German Cancer Research Center)H-Index: 16
view all 24 authors...
Chromothripsis and chromoanasynthesis are catastrophic events leading to clustered genomic rearrangements. Whole-genome sequencing revealed frequent complex genomic rearrangements (n = 16/26) in brain tumors developing in mice deficient for factors involved in homologous-recombination-repair or non-homologous-end-joining. Catastrophic events were tightly linked to Myc/Mycn amplification, with increased DNA damage and inefficient apoptotic response already observable at early postnatal stages. In...
32 CitationsSource