Aleksandra Czyrek
University of Wrocław
Receptor tyrosine kinaseBinding siteBiological activityProtein–protein interactionBiophysicsInternal medicineEndocrinologySomatic cellG protein-coupled receptorInternalizationMutantMembrane proteinEmbryonic stem cellAutophosphorylationCell signalingIntegral membrane proteinCell fate determinationReceptorAdipose tissueChemistryPhage displayFructose 1,6-bisphosphataseApoptosisFGF1IntegrinConjugateCell surface receptorMesenchymal stem cellImmunologySide chainLong-term potentiationSuperoxide dismutaseGrowth factorOxidative stressLTP inductionMoietyEquine metabolic syndromeFluorophoreAlkynePeptideCycloadditionBone marrowMetforminFibroblastBorageMonomethyl auristatin EMechanism (sociology)BoragoEndocytosisStem cellExtracellularMitochondrionBALB 3T3 CellsCell agingMemory formationEthyl esterIntegrin bindingFood scienceTyrosine kinaseFibroblast growth factor receptor 1Cancer researchAmino acidPlasma protein bindingEffectorSenescenceCyclic peptideCell growthSignal transductionStereochemistryFibroblast growth factorNAD+ kinaseProtein moonlightingFibroblast growth factor receptorCell divisionBiologyCell biologyCancer cell
Publications 12
#1Natalia Porębska (UWr: University of Wrocław)H-Index: 3
#2Marta Pozniak (UWr: University of Wrocław)H-Index: 1
Last. Lukasz Opalinski (UWr: University of Wrocław)H-Index: 3
view all 10 authors...
Abstract Fibroblast growth factor receptors (FGFRs) are integral membrane proteins involved in various biological processes including proliferation, migration and apoptosis. There are a number of regulatory mechanisms of FGFR signaling, which tightly control the specificity and duration of transmitted signals. The effect of the FGFRs spatial distribution in the plasma membrane on receptor-dependent functions is still largely unknown. We have demonstrated that oligomerization of FGF1 with coiled-...
1 CitationsSource
#1Martyna SochackaH-Index: 2
#2Lukasz OpalinskiH-Index: 3
Last. Malgorzata ZakrzewskaH-Index: 17
view all 8 authors...
#1Przemysław Duda (UWr: University of Wrocław)H-Index: 5
#2Tomasz Wójtowicz (Nencki Institute of Experimental Biology)H-Index: 13
Last. Dariusz Rakus (UWr: University of Wrocław)H-Index: 25
view all 7 authors...
Long-term potentiation (LTP) is a molecular basis of memory formation. Here, we demonstrate that LTP critically depends on fructose 1,6-bisphosphatase 2 (Fbp2)—a glyconeogenic enzyme and moonlighting protein protecting mitochondria against stress. We show that LTP induction regulates Fbp2 association with neuronal mitochondria and Camk2 and that the Fbp2–Camk2 interaction correlates with Camk2 autophosphorylation. Silencing of Fbp2 expression or simultaneous inhibition and tetramerization of the...
4 CitationsSource
#1Przemysław DudaH-Index: 5
#2Tomasz WójtowiczH-Index: 13
Last. Dariusz RakusH-Index: 25
view all 7 authors...
#1Martyna Sochacka (UWr: University of Wrocław)H-Index: 2
#2Lukasz Opalinski (UWr: University of Wrocław)H-Index: 3
Last. Malgorzata Zakrzewska (UWr: University of Wrocław)H-Index: 17
view all 8 authors...
Fibroblast growth factors (FGFs) via their receptors (FGFRs) transduce signals from the extracellular space to the cell interior, modulating pivotal cellular processes such as cell proliferation, motility, metabolism and death. FGF superfamily includes a group of fibroblast growth factor homologous factors (FHFs), proteins whose function is still largely unknown. Since FHFs lack the signal sequence for secretion and are unable to induce FGFR-dependent cell proliferation, these proteins were cons...
6 CitationsSource
#1Anna Szlachcic (UWr: University of Wrocław)H-Index: 10
#2Martyna Sochacka (UWr: University of Wrocław)H-Index: 2
Last. Malgorzata Zakrzewska (UWr: University of Wrocław)H-Index: 17
view all 7 authors...
Fibroblast growth factor 1 (FGF1) has been shown to interact with integrin αvβ3 through a specific binding site, involving Arg35 residue. The FGF1 mutant (R35E) with impaired integrin binding was found to be defective in its proliferative response, although it was still able to interact with FGF receptors (FGFR) and heparin and induce the activation of downstream signaling pathways. Here, we demonstrate that the lack of mitogenic potential of R35E mutant is directly caused by its decreased therm...
4 CitationsSource
#1Marta Latko (UWr: University of Wrocław)H-Index: 3
#2Aleksandra Czyrek (UWr: University of Wrocław)H-Index: 6
Last. Łukasz Opaliński (UWr: University of Wrocław)H-Index: 7
view all 7 authors...
Fibroblast growth factors (FGFs) and their receptors (FGFRs) constitute signaling circuits that transmit signals across the plasma membrane, regulating pivotal cellular processes like differentiation, migration, proliferation, and apoptosis. The malfunction of FGFs/FGFRs signaling axis is observed in numerous developmental and metabolic disorders, and in various tumors. The large diversity of FGFs/FGFRs functions is attributed to a great complexity in the regulation of FGFs/FGFRs-dependent signa...
26 CitationsSource
#1Magdalena Lipok (UWr: University of Wrocław)H-Index: 1
#2Anna Szlachcic (UWr: University of Wrocław)H-Index: 10
Last. Jacek Otlewski (UWr: University of Wrocław)H-Index: 44
view all 5 authors...
: Overexpression of fibroblast growth factor receptor 1 (FGFR1) is a common aberration in lung and breast cancers and has necessitated the design of drugs targeting FGFR1-dependent downstream signaling and FGFR1 ligand binding. To date, the major group of drugs being developed for treatment of FGFR1-dependent cancers are small-molecule tyrosine kinase inhibitors; however, the limited specificity of these drugs has led to increasing attempts to design molecules targeting the extracellular domain ...
8 CitationsSource
#1K.W. Swiderska (UWr: University of Wrocław)H-Index: 1
#2Anna Szlachcic (UWr: University of Wrocław)H-Index: 10
Last. Jacek Otlewski (UWr: University of Wrocław)H-Index: 44
view all 5 authors...
Abstract Recent advances in site-specific protein modification include the increasingly popular incorporation of unnatural amino acid(s) using amber codon, a method developed by Schultz and coworkers. In this study, we employ this technique to introduce propargyllysine (PrK) in human fibroblast growth factor 2 (FGF2). Owing to an alkyne moiety in its side chain, PrK is compatible with Cu(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition (CuAAC). We successfully tested CuAAC-mediated conjugatio...
9 CitationsSource
#1Krzysztof Marycz (Wroclaw University of Environmental and Life Sciences)H-Index: 29
#2Katarzyna Kornicka (Wroclaw University of Environmental and Life Sciences)H-Index: 16
Last. Aleksandra CzyrekH-Index: 6
view all 4 authors...
Currently, equine metabolic syndrome (EMS), an endocrine disease linked to insulin resistance, affects an increasing number of horses. However, little is known about the effect of EMS on mesenchymal stem cells that reside in adipose tissue (ASC). Thus it is crucial to evaluate the viability and growth kinetics of these cells, particularly in terms of their application in regenerative medicine. In this study, we investigated the proliferative capacity, morphological features, and accumulation of ...
55 CitationsSource