David Scully
Hull York Medical School
Regenerative medicineInternal medicineEndocrinologyRegeneration (biology)ChemistryMyocyteEx vivoMyoDSkeletal musclePlatelet-rich plasmaStem cellMyoblast proliferationCardiotoxinMyogenesisC2C12MedicineCell cultureBiologyPlateletCell biology
9Publications
3H-index
43Citations
Publications 9
Newest
#1Joseph Barlow (Hull York Medical School)
#2Pagona Panagiota Sfyri (Hull York Medical School)H-Index: 2
Last. Antonios Matsakas (Hull York Medical School)H-Index: 18
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NEW FINDINGS What is the central question of this study? What is the impact of obesity-independent hyperlipidaemia on skeletal muscle stem cell function of ApoE-deficient (ApoE-/- ) mice? What is the main finding and its importance? Compromised muscle stem cell function accounts for the impaired muscle regeneration in hyperlipidaemic ApoE-/- mice. Importantly, impaired muscle regeneration is normalised by administration of platelet releasate. ABSTRACT Muscle satellite cells are important stem ce...
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#1Sandrine Verpoorten (Hull York Medical School)H-Index: 3
#2Peggy Sfyri (Hull York Medical School)H-Index: 4
Last. Antonios Matsakas (Hull York Medical School)H-Index: 18
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Aim: The prevalence of obesity is a major risk factor for cardiovascular and metabolic diseases including impaired skeletal muscle regeneration. Since skeletal muscle regenerative capacity is regulated by satellite cells, we aimed to investigate whether a high-fat diet impairs satellite cell function and whether this is linked to fatty acid uptake via CD36. We also aimed to determine whether loss of CD36 impacts on muscle redox homeostasis and skeletal muscle regenerative capacity. Methods: We s...
3 CitationsSource
#1David Scully (Hull York Medical School)H-Index: 3
#2Peggy Sfyri (Hull York Medical School)H-Index: 4
Last. Antonios MatsakasH-Index: 18
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Promoting cell proliferation is the cornerstone of most tissue regeneration therapies. As platelet‐based applications promote cell division and can be customised for tissue‐specific efficacy, this makes them strong candidates for developing novel regenerative therapies. Therefore, the aim of this study was to determine if platelet releasate could be optimised to promote cellular proliferation and differentiation of specific tissues. Growth factors in platelet releasate were profiled for physiolo...
3 CitationsSource
#1Antonios Matsakas (Hull York Medical School)H-Index: 18
#2David Scully (Hull York Medical School)H-Index: 3
Last. Matthew J. HardmanH-Index: 34
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Aim Restoration of dysfunctional tissue using platelet-based applications has become an attractive field of research. Due to the cost and safety of such approaches to deliver growth factors in injured tissue; many studies have investigated their potential in regenerative medicine. However, the mechanisms involved in platelet-mediated regeneration are currently poorly understood. The aim of this study was to determine the effect of platelets on cardiac and skeletal myoblast proliferation and diff...
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#1Antonios Matsakas (Hull York Medical School)H-Index: 18
#2Joseph Barlow (Hull York Medical School)
Last. David Scully (Hull York Medical School)H-Index: 3
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Introduction Apolipoprotein E deficient (ApoE KO) mice are an established model of hyperlipidemia and atherosclerosis, which displays increased oxidative stress and triglyceride contents in the skeletal muscle. Experimental evidence suggests that ApoE KO mice have impaired regeneration after injury. Skeletal muscle stem cells are responsible for the regenerative capacity of skeletal muscle and undergo activation, proliferation and differentiation upon injury in order to support muscle repair and...
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#1David Scully (Hull York Medical School)H-Index: 3
#2Antonios Matsakas (Hull York Medical School)H-Index: 18
Platelet-based applications are currently used for the delivery of growth factors and other biomolecules as autologous biomaterials in regenerative medicine and cosmetic therapies. Many studies have revealed that platelet-based applications such as platelet-rich plasma and platelet releasate exhibit beneficial biological effects after a sports injury or trauma when administered locally by intramuscular injections. At present, treatment of the public, patients and athletes with platelet-based app...
3 CitationsSource
#1David Scully (Hull York Medical School)H-Index: 3
#2Peggy Sfyri (Hull York Medical School)H-Index: 4
Last. Antonios Matsakas (Hull York Medical School)H-Index: 18
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Aim: The use of platelets as biomaterials has gained intense research interest. However, the mechanisms regarding platelet-mediated skeletal myogenesis remain to be established. The aim of this study was to determine the role of platelet releasate in skeletal myogenesis and muscle stem cell fate in vitro and ex vivo respectively. Methods: We analysed the effect of platelet releasate on proliferation and differentiation of C2C12 myoblasts by means of cell proliferation assays, immunohistochemistr...
11 CitationsSource
#1David Scully (Hull York Medical School)H-Index: 3
#2Khalid M. Naseem (University of Leeds)H-Index: 23
Last. Antonios Matsakas (Hull York Medical School)H-Index: 18
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: Platelet-based applications such as platelet-rich plasma (PRP) and platelet releasate have gained unprecedented attention in regenerative medicine across a variety of tissues as of late. The rationale behind utilizing PRP originates in the delivery of key cytokines and growth factors from α-granules to the targeted area, which in turn act as cell cycle regulators and promote the healing process across a variety of tissues. The aim of the present review is to assimilate current experimental evi...
16 CitationsSource
#1David ScullyH-Index: 3
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