Jingheng Zhou
National Institutes of Health
Protein subunitBiophysicsDownregulation and upregulationG protein-coupled receptorDopaminergicHippocampal formationEndoplasmic reticulumEndogenyNeuroscienceReceptorHEK 293 cellsMembrane potentialChemistryMonoamine neurotransmitterGatingModel organismIn vivoProteasomeKainic acidConformational changeEpilepsyLocus coeruleusNorepinephrineXenopusTemporal lobeExtracellularFish <Actinopterygii>Classical conditioningDopamineGene isoformBiochemistryHsp70Premovement neuronal activityPotassium channelBiologyFluorescenceCell biologyFörster resonance energy transfer
19Publications
9H-index
519Citations
Publications 18
Newest
#1Weiting Zhang (UNC: University of North Carolina at Chapel Hill)
#2Tzu-Hao Harry Chao (UNC: University of North Carolina at Chapel Hill)
Last. Hongtu Zhu (UNC: University of North Carolina at Chapel Hill)H-Index: 67
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Fiber-photometry is an emerging technique for recording fluorescent sensor activity in the brain. Here we demonstrate significant hemoglobin-absorption artifacts that may be misinterpreted as sensor activity changes. Because hemoglobin exists in nearly every location in the brain and its concentration varies over time, such artifacts could significantly impede the accuracy of photometry recording results. We present a novel use of spectral photometry technique and propose a computational method ...
Source
#1Fangmiao Sun (PKU: Peking University)H-Index: 8
#2Jingheng Zhou (NIH: National Institutes of Health)H-Index: 9
Last. Yulong Li (PKU: Peking University)H-Index: 21
view all 16 authors...
Dopamine (DA) plays a critical role in the brain, and the ability to directly measure dopaminergic activity is essential for understanding its physiological functions. We therefore developed red fluorescent G-protein-coupled receptor-activation-based DA (GRABDA) sensors and optimized versions of green fluorescent GRABDA sensors. In response to extracellular DA, both the red and green GRABDA sensors exhibit a large increase in fluorescence, with subcellular resolution, subsecond kinetics and nano...
36 CitationsSource
#1Fangmiao Sun (PKU: Peking University)H-Index: 8
#2Jingheng Zhou (NIH: National Institutes of Health)H-Index: 9
Last. Yulong Li (PKU: Peking University)H-Index: 21
view all 15 authors...
The monoamine neuromodulator dopamine (DA) plays a critical role in the brain, and the ability to directly measure dopaminergic activity is essential for understanding its physiological functions. We therefore developed the first red fluorescent GPCR-activation‒based DA (GRABDA) sensors and optimized versions of green fluorescent GRABDA sensors following our previous studies. In response to extracellular DA, both the red and green GRABDA sensors have a large increase in fluorescence (ΔF/F0 value...
15 CitationsSource
#1Yizhou ZhuoH-Index: 2
#2Fangmiao SunH-Index: 8
Last. Yulong LiH-Index: 21
view all 10 authors...
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#1Jiesi Feng (PKU: Peking University)H-Index: 10
#2Zhang C (CAS: Chinese Academy of Sciences)H-Index: 1
Last. Yulong LiH-Index: 21
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Summary Norepinephrine (NE) is a key biogenic monoamine neurotransmitter involved in a wide range of physiological processes. However, its precise dynamics and regulation remain poorly characterized, in part due to limitations of available techniques for measuring NE in vivo. Here, we developed a family of GPCR activation-based NE (GRABNE) sensors with a 230% peak ΔF/F0 response to NE, good photostability, nanomolar-to-micromolar sensitivities, sub-second kinetics, and high specificity. Viral- o...
130 CitationsSource
#1Fang Hu (Qingdao University)H-Index: 1
#2Jingheng Zhou (PKU: Peking University)H-Index: 9
Last. KeWei Wang (Qingdao University)H-Index: 1
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Summary The heat shock protein 70 (Hsp70) is upregulated in response to stress and has been implicated as a stress marker in temporal lobe epilepsy (TLE). However, whether Hsp70 plays a pathologic or protective role in TLE remains unclear. Here we report a deleterious role of Hsp70 in kainic acid (KA)-induced seizures. Hsp70 expression is upregulated in a KA model of TLE, and silencing or inhibition of Hsp70 suppresses neuronal hyperexcitability and attenuates acute or chronic epilepsy by enhanc...
10 CitationsSource
#1Fangmiao Sun (PKU: Peking University)H-Index: 8
#2Jianzhi Zeng (PKU: Peking University)H-Index: 9
Last. Yulong Li (PKU: Peking University)H-Index: 21
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Summary Dopamine (DA) is a central monoamine neurotransmitter involved in many physiological and pathological processes. A longstanding yet largely unmet goal is to measure DA changes reliably and specifically with high spatiotemporal precision, particularly in animals executing complex behaviors. Here, we report the development of genetically encoded GPCR-activation-based-DA (GRAB DA ) sensors that enable these measurements. In response to extracellular DA, GRAB DA sensors exhibit large fluores...
264 CitationsSource
#1Chengbo Meng (NIH: National Institutes of Health)H-Index: 1
#2Jingheng Zhou (NIH: National Institutes of Health)H-Index: 9
Last. Guohong Cui (NIH: National Institutes of Health)H-Index: 11
view all 6 authors...
Summary To achieve simultaneous measurement of multiple cellular events in molecularly defined groups of neurons in vivo , we designed a spectrometer-based fiber photometry system that allows for spectral unmixing of multiple fluorescence signals recorded from deep brain structures in behaving animals. Using green and red Ca 2+ indicators differentially expressed in striatal direct- and indirect-pathway neurons, we were able to simultaneously monitor the neural activity in these two pathways in ...
52 CitationsSource
Neuromodulation of neural networks, whereby a selected circuit is regulated by a particular modulator, plays a critical role in learning and memory. Among neuromodulators, acetylcholine (ACh) plays a critical role in hippocampus-dependent memory and has been shown to modulate neuronal circuits in the hippocampus. However, it has remained unknown how ACh modulates hippocampal output. Here, using in vitro and in vivo approaches, we show that ACh, by activating oriens lacunosum moleculare (OLM) int...
31 CitationsSource
#1Fang Hu (Qingdao University)H-Index: 1
#2Jingheng Zhou (PKU: Peking University)H-Index: 9
Last. KeWei Wang (PKU: Peking University)H-Index: 22
view all 8 authors...
The heat shock protein 70 (Hsp70) is upregulated in response to stress and has been implicated as a stress marker in temporal lobe epilepsy (TLE). However, whether Hsp70 plays a pathologic or protective role in TLE remains unclear. Here we report that Hsp70 exerts an unexpected deleterious role in kainic acid (KA)-induced seizures, and inhibition of Hsp70 suppresses neuronal hyperexcitability and attenuates both acute and chronic seizures via enhancing A-type potassium currents primarily formed ...
1 CitationsSource