Clara Nassrin Kriebel
Goethe University Frankfurt
Binding siteChemical shiftDeprotonationDark stateKineticsSolid-state nuclear magnetic resonanceInfrared spectroscopyProtomerChemistryRhodopsinIon pumpPolyeneContext (language use)Retinal bindingProtonationPeptide bondSodiumNuclear magnetic resonance spectroscopyPhotochemistryCrystallographyIon transporter
Publications 3
#1Orawan Jakdetchai (Goethe University Frankfurt)H-Index: 2
#2Peter Eberhardt (Goethe University Frankfurt)H-Index: 4
Last. Clemens Glaubitz (Goethe University Frankfurt)H-Index: 35
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The functional mechanism of the light-driven sodium pump Krokinobacter eikastus rhodopsin 2 (KR2) raises fundamental questions since the transfer of cations must differ from the better-known principles of rhodopsin-based proton pumps. Addressing these questions must involve a better understanding of its photointermediates. Here, dynamic nuclear polarization-enhanced solid-state nuclear magnetic resonance spectroscopy on cryo-trapped photointermediates shows that the K-state with 13-cis retinal d...
#1Jagdeep Kaur (Goethe University Frankfurt)H-Index: 5
#2Clara Nassrin Kriebel (Goethe University Frankfurt)H-Index: 2
Last. Clemens Glaubitz (Goethe University Frankfurt)H-Index: 35
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Abstract Krokinobacter eikastus rhodopsin 2 (KR2) is a pentameric, light-driven ion pump, which selectively transports sodium or protons. The mechanism of ion selectivity and transfer is unknown. By using conventional as well as dynamic nuclear polarization (DNP)-enhanced solid-state NMR, we were able to analyse the retinal polyene chain between positions C10 and C15 as well as the Schiff base nitrogen in the KR2 resting state. In addition, 50% of the KR2 13C and 15N resonances could be assigned...
13 CitationsSource
#1Marvin Asido (Goethe University Frankfurt)H-Index: 1
#2Peter Eberhardt (Goethe University Frankfurt)H-Index: 4
Last. Josef Wachtveitl (Goethe University Frankfurt)H-Index: 49
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We report a comparative study on the structural dynamics of the light-driven sodium pump Krokinobacter eikastus rhodopsin 2 wild type under sodium and proton pumping conditions by means of time-resolved IR spectroscopy. The kinetics of KR2 under sodium pumping conditions exhibits a sequential character, whereas the kinetics of KR2 under proton pumping conditions involves several equilibrium states. The sodium translocation itself is characterized by major conformational changes of the protein ba...
7 CitationsSource