Shiqian Fu
Northeast Agricultural University
Linear rangeGeneColloidal goldDetection limitTransduction (genetics)ChromogenicAptamerOligonucleotideOrganismNuclear chemistryAnimal scienceBiotechnologyMolecular biologyEnumerationNaked eyePesticideDoseEnzymeChemistryConjugateCombinatorial chemistryNucleic acidQuechersRaw milkStreptavidinCronobacter sakazakiiCarbonyl ironFluorescein isothiocyanateLactobacillus acidophilusSingle-strand DNA-binding proteinBiotinHydroxynaphthol blueNanoclustersPathogenic bacteriaInfant formulaStaphylococcus aureusAcetochlorSalmonella entericaCronobacterMagnetic separationDipstickImmunomagnetic separationSalmonellaMultiplexExonuclease IIIFilter paperCronobacter speciesPositive correlationStress resistanceColorimetric sensorEnvironmental stress responsePure cultureTreatment periodDietary intakeLinear relationshipCulture independentPaper basedFood scienceChromatographyReal-time polymerase chain reactionCarbonDesiccationPrimer dimerBacteriaEscherichia coliDNALoop-mediated isothermal amplificationPrimer (molecular biology)Multilocus sequence typingDNA extractionAmpliconZeta potentialBiologyFood processingFluorescenceNanocompositeAbsorbance
12Publications
5H-index
53Citations
Publications 15
Newest
#1Lihan Wang (NEAU: Northeast Agricultural University)H-Index: 2
#2Xinyi Pang (Nanjing University of Finance and Economics)
Last. Chaoxin Man (NEAU: Northeast Agricultural University)H-Index: 8
view all 11 authors...
Abstract null null Cronobacter sakazakii (C. sakazakii) is a food-borne pathogen that can be spread by powdered infant formula (PIF) to cause illness in infants. Bacteriophages (phages) are an effective potential strategy to control food-borne pathogens. Phage JK004 which isolated from the native environment of C. sakazakii was a novel virulent phage with short latent period and large burst size. JK004 had tolerance to heat, pH and osmotic-stress. The phage JK004 did not contain known virulence,...
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#1Xinyan Yang (NEAU: Northeast Agricultural University)H-Index: 5
#2Lihan Wang (NEAU: Northeast Agricultural University)H-Index: 2
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 8 authors...
Abstract null null A novel fluorescent platform of DNA-stabilized silver nanoclusters (DNA-AgNCs) has been developed based on exonuclease III (Exo III) amplification-assisted for simple and sensitive detection of Salmonella Typhimurium (S. Typhimurium). The platform was designed by using magnetic beads, aptamer, its complementary DNA, hairpin probe (HP), Exo III, AgNO3, and NaBH4. The functionalized HP contained a cytosine-rich oligonucleotide loop (C-rich loop), which served as an effective tem...
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#1Lihan Wang (NEAU: Northeast Agricultural University)H-Index: 2
#2Stephen Forsythe (University of Nottingham)H-Index: 6
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 6 authors...
ABSTRACT null null Members of the Cronobacter genus include food-borne pathogens that can cause infections in infants, with a mortality rate as high as 40 to 80%. The high fatality rate of Cronobacter and its isolation from numerous types of food, especially from powdered infant formula, demonstrate the serious nature of this organism. The source tracking of Cronobacter spp. and the analysis of high-frequency species from different sources are helpful for a more targeted control. Furthermore, th...
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#1Yuwei Ren (NEAU: Northeast Agricultural University)H-Index: 1
#2Pingping Gao (NEAU: Northeast Agricultural University)H-Index: 1
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 11 authors...
ABSTRACT Escherichia coli O157:H7 (E. coli O157:H7), one of the most widespread foodborne pathogens, can cause a series of diseases and even lead to death. In this study, a highly sensitive method was developed by combining aptamer-exonuclease III (Exo III)–assisted amplification with lateral flow assay (LFA) based on gold nanoparticles (AuNP). The compound of single-stranded (ss) DNA-anti-E. coli O157:H7 aptamer (ssDNA-aptamer) was formed by hybridization between designed target ssDNA and aptam...
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#1Tao Yang (NEAU: Northeast Agricultural University)H-Index: 3
#2Zhenghui Wang (NEAU: Northeast Agricultural University)H-Index: 2
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 10 authors...
Effective testing tools for Escherichia coli O157:H7 can prevent outbreaks of foodborne illness. In this paper, a smartphone-based colorimetric aptasensor was developed using functionalized gold nanoparticles (GNP) and multi-walled carbon nanotubes (MWCNT) for monitoring E. coli O157:H7 in milk. The maximum absorption peak of GNP bonded with aptamer (Apt) generated evident transformation from 518 to 524 nm. The excess GNP-Apt was removed by functionalized MWCNT magnetized with carbonyl iron powd...
Source
#1Pingping GaoH-Index: 1
#2Lihan WangH-Index: 2
Last. Yujun JiangH-Index: 8
view all 10 authors...
In this paper, a novel and ultrasensitive lateral flow assay (LFA) based on aptamer–magnetic separation, and multifold Au nanoparticles (AuNPs) was developed for visual detecting Salmonella enterica ser. Typhimurium (S. Typhimurium). The method realized magnetic enrichment and signal transduction via magnetic separation and achieved signal amplification through hybridizing AuNPs–capture probes and AuNPs–amplification probes to form multifold AuNPs. Two different thiolated single-strand DNA (ssDN...
Source
#1Shiqian Fu (NEAU: Northeast Agricultural University)H-Index: 5
#2Xue Qin (NEAU: Northeast Agricultural University)H-Index: 3
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 9 authors...
Due to the lack of specific genes for rapid detection methods of Cronobacter sakazakii in food samples, whole genome sequence analysis was performed in this investigation using the basic local alignment search tool. Forty-two DNA fragments unique to C. sakazakii were mined, then primers were designed and screened by PCR and loop-mediated isothermal amplification (LAMP). Two primer sets, CS1 and CS31, were found as specific and stable primers, with their corresponding nucleic acid targets the CSK...
Source
#1Xue Qin (NEAU: Northeast Agricultural University)H-Index: 3
#2Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 13
Last. Wei Zhang (NEAU: Northeast Agricultural University)H-Index: 6
view all 12 authors...
Acetochlor has been widely used globally for its effective weed control, but the dietary intake of associated residues by people has become a major concern nowadays. Milk is regarded as the best solvent to dissolve pesticides due to its fat-rich characteristic. In this study, we aimed to evaluate the transfer of acetochlor from feed to raw milk. Twenty lactating Australian Holstein cows were randomly chosen and divided into 1 control group and 3 treatment groups, feeding acetochlor at the dosage...
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#1Tao Yang (NEAU: Northeast Agricultural University)H-Index: 3
#2Xinyan Yang (NEAU: Northeast Agricultural University)H-Index: 5
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 11 authors...
ABSTRACT Escherichia coli O157:H7 is an extremely serious foodborne pathogen accounting for a vast number of hospitalizations. In this system, a simple, rapid, and safe compound method was developed based on carbonyl iron powder (CIP) and multiwalled carbon nanotubes (MWCNT). Then, the CIP@MWCNT-based aptasensor was constructed by strong π-stacking between nanocomposite and aptamer, single-strand DNA, causing fluorescent quenching of the dye-labeled aptamer. The restoration of dye fluorescence c...
Source
#1Sihan Chen (NEAU: Northeast Agricultural University)H-Index: 5
#2Xinyan Yang (NEAU: Northeast Agricultural University)H-Index: 5
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 7 authors...
Abstract A sensitive and accurate strategy was presented based on gold nanoparticles (AuNPs) for visual detecting viable Salmonella typhimurium (S. typhimurium). In this paper, dual aptamers were used to capture S. typhimurium and PCR amplification, respectively. The highly stable network structure formed by the hybridization between amplicons and AuNPs-probes protects AuNPs from self-aggregation and color change in the presence of salt. Under optimized conditions, the ratio of the absorbance in...
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