Jeffrey J. Yourick
United States Department of the Army
PathologyMolecular biologyCytotoxic T cellNeurotrophic factorsEnzymeChemistryIn vitroIn vivoAcetylcholineOxidative stressProteaseInhalationLungGuinea pigAcetylcholinesteraseSuperior cervical ganglionAchéErythemaHairlessRespiratory tractPlasminogen activatorNicotinamideInhalation exposureSomanSulfur mustardEdemaElastaseAtelectasisProteolysisSkin blistersNiacinamideMicrovesicleNAD+ ADP-RibosyltransferaseHuman PathologyToxicityNecrosisRespiratory systemBiochemistryMedicineNAD+ kinaseCell cultureBiologyMechanism (biology)Pharmacology
Publications 7
#1William J. SmithH-Index: 3
#1William J. SmithH-Index: 3
Last. Jeffrey J. YourickH-Index: 5
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Sulfur mustard (HD) is an alkylating agent that has been shown to have mutagenic, cytotoxic, and vesicating properties. Its use in combat situations has resulted in lethal, incapacitating, and disfiguring injuries. The principal incapacitating injuries come from the vesicating capacity of HD, i.e., production of skin blisters (1). Despite decades of medical research, the mechanism by which HD induces vesication is not known, and no effective antidotes are currently available. The studies in this...
1 CitationsSource
#1Dana R. AndersonH-Index: 25
#2Jeffrey J. Yourick (DA: United States Department of the Army)H-Index: 1
Last. Susan L. ByersH-Index: 5
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AbstractAlthough the majority of deaths resulting from exposure to sulfur mustard (HD) have been due to pulmonary dysfunction, there are no detailed accounts of the pathogenesis of HD-induced lesions in the respiratory tract. Accordingly, we investigated the early changes within the trachea and lungs of rats following inhalation exposure to HD. Anesthetized rats were exposed by intratracheal intubation to vaporized HD (0.35 mg in 100 m l absolute ethanol) or ethanol alone for 50 min. Animals wer...
41 CitationsSource
AbstractThe pathologic mechanisms underlying sulfur mustard (HD)-induced skin vesication are as yet undefined. Enhanced proteolysis activity has been postulated as the cause of HD-induced dermal epidermal separation. Using a chromogenic assay, we previously reported that HD-exposed hairless guinea pig skin biopsies demonstrated increased proteolysis of the peptide substrate Tosyl-gly-pro-arg-p-nitroanilide, Chromozym TH (TH). Elastaise activity has been used as a biomarker of inflammation in ski...
19 CitationsSource
#1Jeffrey J. YourickH-Index: 5
#1Jeffrey J. YourickH-Index: 9
Last. Larry W. MitcheltreeH-Index: 12
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Abstract Developing skin lesions on hairless guinea pigs due to 2,2′-dichlorodiethyl sulfide (sulfur mustard, HD) exposure were examined to determine the time course for the appearance of histopathologic markers in relationship to skin NAD + and NADP + content after HD exposure. Hairless guinea pig skin was exposed to HD for 8 min by means of a vapor cup. Skin punches were taken at 1, 2, 4, 8, 12, 16, 20 and 24 h after HD exposure. Intracellular edema (IE) appeared at 2 h and increased steadily ...
23 CitationsSource
#1Fred M. CowanH-Index: 9
#2J. J. YourickH-Index: 1
Last. William J. SmithH-Index: 20
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The pathologic mechanisms underlying sulfur mustard (HD)-induced skin vesication are as yet undefined. Papirmeister et al. (1985) postulate enhanced proteolytic activity as a proximate cause of HD-induced cutaneous injury. Using a chromogenic peptide substrate assay, we previously reported that in vitro exposure of cell cultures to HD enhances proteolytic activity. We have continued our investigation of HD-increased proteolytic activity in vitro and have expanded our studies to include an in viv...
43 CitationsSource
Abstract It has been postulated that sulfur mustard (HD) damage may activate poly(ADP-ribose) polymerase (PADPRP), resulting in depletion of cellular NAD + . This biochemical alteration is postulated to result in blister (vesicle) formation. It has been previously demonstrated that niacinamide (NAM), an inhibitor of PADPRP and a precursor for NAD + synthesis, may be useful as a pretreatment compound to reduce HD-induced microvesication. The present study was undertaken to determine whether niaci...
23 CitationsSource
#1Jeffrey J. YourickH-Index: 8
#1Jeffrey J. Yourick (DA: United States Department of the Army)H-Index: 5
Last. Radharaman RayH-Index: 1
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Acetylcholinesterase (AChE) in the clonal NG108-15 cell line has been previously characterized. This cell line represents an in vitro system to study AChE regulation and effects of chemical compounds that may alter AChE activity. Recently, glycyl-L-glutamine (GLG) was demonstrated to function as a neurotrophic factor for maintenance of AChE content in cat denervated superior cervical ganglion cells. In the present study, regeneration of AChE activity in cultures of undifferentiated NG108-15 cell...
1 CitationsSource