Eva Hitz
University of Basel
Protein subunitGenePhosphorylationMutantCasein kinase 2Cell fate determinationConditional gene knockoutGene knockdownKinaseCytoplasmImmunologyWild typeAxonemePlasmodium bergheiMalariaPlasmodium falciparumLysophosphatidylcholinePlasmodiumGametogenesisMutationGeneticsMotilitySexual differentiationParasite hostingDNA replicationTranscription factorBiologyActivator (genetics)Heterochromatin protein 1Cell biologyNonsense mutationProtein kinase ACRISPRGametocyte
6Publications
3H-index
138Citations
Publications 6
Newest
#1Eva Hitz (University of Basel)H-Index: 3
#2Natalie Wiedemar (University of Basel)H-Index: 8
Last. Till S. Voss (University of Basel)H-Index: 25
view all 7 authors...
Cyclic AMP (cAMP) signalling is crucial for the propagation of asexual malaria blood stage parasites. Recent work on Plasmodium falciparum demonstrated that phosphorylation of the invasion ligand AMA1 by the catalytic subunit of cAMP-dependent protein kinase A (PfPKAc) is an essential step during parasite invasion into red blood cells. However, the exact mechanisms regulating PfPKAc activity are only partially understood and PfPKAc function has not been extensively studied in gametocytes, the se...
Source
#1Marta Tibúrcio (Francis Crick Institute)H-Index: 3
#2Eva Hitz (Swiss TPH: Swiss Tropical and Public Health Institute)H-Index: 3
Last. Moritz Treeck (Francis Crick Institute)H-Index: 23
view all 9 authors...
Malaria is a mosquito-borne disease caused by apicomplexan parasites of the genus Plasmodium. Completion of the parasite's life cycle depends on the transmission of sexual stages, the gametocytes, from an infected human host to the mosquito vector. Sexual commitment occurs in only a small fraction of asexual blood-stage parasites and is initiated by external cues. The gametocyte development protein 1 (GDV1) has been described as a key facilitator to trigger sexual commitment. GDV1 interacts with...
Source
#1Eva Hitz (University of Basel)H-Index: 3
#2Olivia Grüninger (University of Basel)
Last. Till S. Voss (University of Basel)H-Index: 25
view all 9 authors...
Casein kinase 2 (CK2) is a pleiotropic kinase phosphorylating substrates in different cellular compartments in eukaryotes. In the malaria parasite Plasmodium falciparum, PfCK2 is vital for asexual proliferation of blood-stage parasites. Here, we applied CRISPR/Cas9-based gene editing to investigate the function of the PfCK2α catalytic subunit in gametocytes, the sexual forms of the parasite that are essential for malaria transmission. We show that PfCK2α localizes to the nucleus and cytoplasm in...
Source
#1Marta Tibúrcio (Francis Crick Institute)H-Index: 3
#2Eva Hitz (University of Basel)H-Index: 3
Last. Moritz Treeck (Francis Crick Institute)H-Index: 23
view all 9 authors...
Abstract Malaria is a mosquito-borne disease caused by apicomplexan parasites of the genus Plasmodium. Completion of the parasite’s life cycle depends on the transmission of sexual stages, the gametocytes, from an infected human host to the mosquito vector. Sexual commitment occurs in only a small fraction of asexual blood stage parasites and is initiated by external cues. The gametocyte development protein 1 (GDV1) has been described as a key facilitator to trigger sexual commitment. GDV1 inter...
3 CitationsSource
#1Eva Hitz (University of Basel)H-Index: 3
#2Aurélia C. Balestra (University of Geneva)H-Index: 3
Last. Till S. Voss (University of Basel)H-Index: 25
view all 4 authors...
In malaria parasites, male gametogenesis is a proliferative stage essential for parasite transmission to the mosquito vector. It is a rapid process involving three rounds of genome replication alternating with closed endomitoses, and assembly of axonemes to produce eight flagellated motile microgametes. Studies in Plasmodium berghei have highlighted tight regulation of gametogenesis by a network of kinases. The P. berghei MAPK homologue PbMAP-2 is dispensable for asexual development but importan...
6 CitationsSource
#1Nicolas M. B. Brancucci (Glas.: University of Glasgow)H-Index: 15
#2Joseph P. Gerdt (Harvard University)H-Index: 8
Last. Matthias Marti (Glas.: University of Glasgow)H-Index: 37
view all 28 authors...
Transmission represents a population bottleneck in the Plasmodium life cycle and a key intervention target of ongoing efforts to eradicate malaria. Sexual differentiation is essential for this process, as only sexual parasites, called gametocytes, are infective to the mosquito vector. Gametocyte production rates vary depending on environmental conditions, but external stimuli remain obscure. Here, we show that the host-derived lipid lysophosphatidylcholine (LysoPC) controls P. falciparum cell fa...
117 CitationsSource