Elizabeth I. Harper
University of Notre Dame
CancerVirologyRNA interferenceExtracellular matrixIn vivoSmall interfering RNATumor microenvironmentAnopheles gambiaeLarvicidePeritoneal cavityMetastasisMesotheliumMosquito controlOvarian tumorOvarian cancerAnophelesReceptor complexCancer researchRNAMedicineBiologyImmune systemCell biology
13Publications
6H-index
152Citations
Publications 9
Newest
Proteases play a crucial role in the progression and metastasis of ovarian cancer. Pericellular protein degradation and fragmentation along with remodeling of the extracellular matrix (ECM) is accomplished by numerous proteases that are present in the ovarian tumor microenvironment. Several proteolytic processes have been linked to cancer progression, particularly those facilitated by the matrix metalloproteinase (MMP) family. These proteases have been linked to enhanced migratory ability, extra...
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#1Yueying Liu (ND: University of Notre Dame)H-Index: 16
#2Jing Yang (ND: University of Notre Dame)H-Index: 6
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 28 authors...
Abstract The majority of women with ovarian cancer are diagnosed with metastatic disease, therefore elucidating molecular events that contribute to successful metastatic dissemination may identify additional targets for therapeutic intervention and thereby positively impact survival. Using two human high grade serous ovarian cancer cell lines with inactive TP53 and multiple rounds of serial in vivo passaging, we generated sublines with significantly accelerated intra-peritoneal (IP) growth. Comp...
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#1Marwa Asem (ND: University of Notre Dame)H-Index: 5
#2Allison Young (ND: University of Notre Dame)H-Index: 2
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 14 authors...
The non-canonical Wnt ligand Wnt5a is found in high concentrations in ascites of women with ovarian cancer. In this study, we elucidated the role of Wnt5a in ovarian cancer metastasis. Wnt5a promoted ovarian tumor cell adhesion to peritoneal mesothelial cells as well as migration and invasion, leading to colonization of peritoneal explants. Host components of the ovarian tumor microenvironment, notably peritoneal mesothelial cells and visceral adipose, secreted Wnt5a. Conditional knockout of hos...
6 CitationsSource
#1Elizabeth I. Harper (ND: University of Notre Dame)H-Index: 6
#2Emma Sheedy (ND: University of Notre Dame)H-Index: 2
Last. M. Stack (ND: University of Notre Dame)H-Index: 3
view all 3 authors...
: Age is one of the biggest risk factors for ovarian cancer. Older women have higher rates of diagnosis and death associated with the disease. In mouse models, it was shown that aged mice had greater tumor burden than their younger counterparts when intraperitoneally injected with ovarian tumor cells. While very few papers have been published looking at the direct link between ovarian cancer metastasis and age, there is a wealth of information on how age affects metastatic microenvironments. Mes...
13 CitationsSource
#1Elizabeth I. Harper (ND: University of Notre Dame)H-Index: 6
#2Elizabeth Loughran (ND: University of Notre Dame)H-Index: 7
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 14 authors...
Ovarian cancer (OvCa) is the deadliest cancer of the female reproductive system, ranking fifth in overall cancer deaths among women. Unlike the majority of cancers, OvCa metastasizes via diffusion through the peritoneal cavity, resulting in multiple metastatic sites, including the omentum and peritoneum. These metastasizing OvCa cells induce rapid mesothelial cell retraction and readily adhere to the sub-mesothelial collagen of the extracellular matrix. Epidemiologic data identifies age as a sig...
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#1Elizabeth Loughran (ND: University of Notre Dame)H-Index: 7
#2Annemarie K. Leonard (ND: University of Notre Dame)H-Index: 2
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 17 authors...
Abstract Ovarian cancer, the most deadly gynecological malignancy in U.S. women, metastasizes uniquely, spreading through the peritoneal cavity and often generating widespread metastatic sites before diagnosis. The vast majority of ovarian cancer cases occur in women over 40 and the median age at diagnosis is 63. Additionally, elderly women receive poorer prognoses when diagnosed with ovarian cancer. Despite age being a significant risk factor for the development of this cancer, there are little...
9 CitationsSource
#1Keshava Mysore (IU: Indiana University)H-Index: 12
#2Limb K. Hapairai (IU: Indiana University)H-Index: 5
Last. Molly Duman-Scheel (IU: Indiana University)H-Index: 16
view all 11 authors...
Background Although larviciding can reduce the number of outdoor biting malaria vector mosquitoes, which may help to prevent residual malaria transmission, the current larvicide repertoire is faced with great challenges to sustainability. The identification of new effective, economical, and biorational larvicides could facilitate maintenance and expansion of the practice of larviciding in integrated malaria vector mosquito control programmes. Interfering RNA molecules represent a novel class of ...
21 CitationsSource
#1Limb K. Hapairai (IU: Indiana University)H-Index: 5
#2Keshava Mysore (IU: Indiana University)H-Index: 12
Last. Molly Duman-Scheel (IU: Indiana University)H-Index: 16
view all 10 authors...
New mosquito control strategies are vitally needed to address established arthropod-borne infectious diseases such as dengue and yellow fever and emerging diseases such as Zika and chikungunya, all of which are transmitted by the disease vector mosquito Aedes aegypti. In this investigation, Saccharomyces cerevisiae (baker’s yeast) was engineered to produce short hairpin RNAs (shRNAs) corresponding to the Aedes aegypti orthologs of fasciculation and elongation protein zeta 2 (fez2) and leukocyte ...
14 CitationsSource
#1Yueying Liu (ND: University of Notre Dame)H-Index: 16
#2Matthew N. Metzinger (ND: University of Notre Dame)H-Index: 3
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 22 authors...
Epithelial ovarian cancer (EOC) is the leading cause of death from gynecologic malignancy, with high mortality attributable to widespread intraperitoneal metastases. Recent meta-analyses report an association between obesity, ovarian cancer incidence, and ovarian cancer survival, but the effect of obesity on metastasis has not been evaluated. The objective of this study was to use an integrative approach combining in vitro , ex vivo , and in vivo studies to test the hypothesis that obesity contr...
48 CitationsSource