Audrey Stoessel
University of Strasbourg
Polyacrylamide gel electrophoresisColloidal goldCytotoxicityConjugated systemImmunotoxinTransduction (genetics)Fusion proteinBiophysicsGenomic libraryDNA damageAntigenReplisomeMolecular biologyAntibodyCovalent bondKinaseChemistryPhage displayCytoplasmMaterials scienceConjugateIn vitroDNA polymerasePeptide libraryToxinIntrabodyTCEPEpidermal growth factor receptorCetuximabPhosphorylated Histone H2AXAntibody DiversityHistone H2AXProgrammed cell deathProliferating cell nuclear antigenProteomicsMonoclonal antibodyEscherichia coliDNABiochemistryDNA replicationChromatinComplementarity determining regionBiologyCell biologyCancer cellBinding selectivityMutagenesis (molecular biology technique)
Publications 7
#1Eric MoeglinH-Index: 2
#2Dominique DesplancqH-Index: 12
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Histone H2AX phosphorylated at serine 139 (γ-H2AX) is a hallmark of DNA damage, signaling the presence of DNA double-strand breaks and global replication stress in mammalian cells. While γ-H2AX can be visualized with antibodies in fixed cells, its detection in living cells was so far not possible. Here, we used immune libraries and phage display to isolate nanobodies that specifically bind to γ-H2AX. We solved the crystal structure of the most soluble nanobody in complex with the phosphopeptide ...
#1Audrey Stoessel (UDS: University of Strasbourg)H-Index: 5
#2Nadja Groysbeck (UDS: University of Strasbourg)H-Index: 2
Last. Mariel Donzeau (UDS: University of Strasbourg)H-Index: 5
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Immunotoxins are emerging candidates for cancer therapeutics. These biomolecules consist of a cell-targeting protein combined to a polypeptide toxin. Associations of both entities can be achieved either chemically by covalent bonds or genetically creating fusion proteins. However, chemical agents can affect the activity and/or stability of the conjugate proteins, and additional purification steps are often required to isolate the final conjugate from unwanted byproducts. As for fusion proteins, ...
#1Nadja Groysbeck (UDS: University of Strasbourg)H-Index: 2
#2Audrey Stoessel (UDS: University of Strasbourg)H-Index: 5
Last. Guy Zuber (UDS: University of Strasbourg)H-Index: 26
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: Therapeutic monoclonal antibodies benefit to patients and the conjugation to gold nanoparticles (AuNPs) might bring additional activities to these macromolecules. However, the behavior of the conjugate will largely depend on the bulkiness of the AuNP and small sizes are moreover preferable for diffusion. Water-soluble thiolate-protected AuNPs having diameters of 2-3 nm can be synthesized with narrow polydispersity and can selectively react with incoming organic thiols via a SN2-like mechanism....
8 CitationsSource
#1Eric Moeglin (UDS: University of Strasbourg)H-Index: 2
#2Dominique Desplancq (UDS: University of Strasbourg)H-Index: 12
Last. Etienne Weiss (UDS: University of Strasbourg)H-Index: 25
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Phosphorylated histone H2AX (γ-H2AX), a central player in the DNA damage response (DDR), serves as a biomarker of DNA double-strand break repair. Although DNA damage is generally visualized by the formation of γ-H2AX foci in injured nuclei, it is unclear whether the widespread uniform nuclear γ-H2AX (called pan-nuclear) pattern occurring upon intense replication stress (RS) is linked to DDR. Using a novel monoclonal antibody that binds exclusively to the phosphorylated C-terminus of H2AX, we dem...
26 CitationsSource
#1Dominique Desplancq (UDS: University of Strasbourg)H-Index: 12
#2Guillaume Freund (UDS: University of Strasbourg)H-Index: 4
Last. Etienne Weiss (UDS: University of Strasbourg)H-Index: 25
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Although chemical inhibition of the DNA damage response (DDR) in cancer cells triggers cell death, it is not clear if the fork blockade achieved with inhibitors that neutralise proteins of the replisome is sufficient on its own to overcome the DDR. Monoclonal antibodies to PCNA, which block the DNA elongation process in vitro, have been developed. When these antibodies were transduced into cancer cells, they are able to inhibit the incorporation of nucleoside analogues. When co-delivered with an...
14 CitationsSource
#1Guillaume Freund (UDS: University of Strasbourg)H-Index: 4
#2Dominique Desplancq (UDS: University of Strasbourg)H-Index: 12
Last. Etienne Weiss (UDS: University of Strasbourg)H-Index: 25
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Intrabodies, when expressed in cells after genetic fusion to fluorescent proteins, are powerful tools to study endogenous protein dynamics inside cells. However, it remains challenging to determine the conditions for specific imaging and precise labelling of the target antigen with such intracellularly expressed antibody fragments. Here, we show that single-chain Fv (scFv) antibody fragments can be generated that specifically recognize proliferating cell nuclear antigen (PCNA) when expressed in ...
8 CitationsSource
#1Pascal Philibert (CNRS: Centre national de la recherche scientifique)H-Index: 25
#2Audrey Stoessel (CNRS: Centre national de la recherche scientifique)H-Index: 5
Last. Pierre Martineau (University of Montpellier)H-Index: 30
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Background Intrabodies are defined as antibody molecules which are ectopically expressed inside the cell. Such intrabodies can be used to visualize or inhibit the targeted antigen in living cells. However, most antibody fragments cannot be used as intrabodies because they do not fold under the reducing conditions of the cell cytosol and nucleus.
89 CitationsSource