Marco Tigano
New York University
GeneNuclear DNAReplication (statistics)DNA repairGenomeDNA damageReplisomeTranslation (biology)In vivoNuclear geneMitochondrial DNAInterferonThymidineMitochondrial biogenesisMitochondrial ribosomeMitochondrionMtDNA replicationDirectionalityDsb repairCellular homeostasisTranscription activator-like effector nucleaseTranscription (biology)EffectorGeneticsDNAMicrohomology-mediated end joiningOrigin of replicationComputational biologyDNA replicationBiologyRibosomeCell biology
11Publications
6H-index
368Citations
Publications 6
Newest
#1Marco Tigano (NYU: New York University)H-Index: 6
#2Danielle C. Vargas (NYU: New York University)H-Index: 1
Last. Agnel Sfeir (NYU: New York University)H-Index: 23
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Mitochondrial DNA double-strand breaks (mtDSBs) are toxic lesions that compromise the integrity of mitochondrial DNA (mtDNA) and alter mitochondrial function1. Communication between mitochondria and the nucleus is essential to maintain cellular homeostasis; however, the nuclear response to mtDSBs remains unknown2. Here, using mitochondrial-targeted transcription activator-like effector nucleases (TALENs)1,3,4, we show that mtDSBs activate a type-I interferon response that involves the phosphoryl...
5 CitationsSource
#1Marco Tigano (NYU: New York University)H-Index: 6
#2Aaron Fraser Phillips (NYU: New York University)
Last. Agnel Sfeir (NYU: New York University)H-Index: 23
view all 3 authors...
Single molecule analysis of replicating DNA (SMARD) is a powerful methodology that allows in vivo analysis of replicating DNA; identification of origins of replication, assessment of fork directionality, and measurement of replication fork speed. SMARD, which has been extensively used to study replication of nuclear DNA, involves incorporation of thymidine analogs to nascent DNA chains and their subsequent visualization through immune detection. Here, we adapt and fine-tune the SMARD technique t...
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#1Yi FuH-Index: 2
#2Marco TiganoH-Index: 6
Last. Agnel SfeirH-Index: 23
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Mitochondria respond to DNA damage and preserve their own genetic material in a manner distinct from that of the nucleus but that requires organized mito–nuclear communication. Failure to resolve mtDNA breaks leads to mitochondrial dysfunction and affects host cells and tissues. Here, we review the pathways that safeguard mitochondrial genomes and examine the insights gained from studies of cellular and tissue-wide responses to mtDNA damage and mito–nuclear genome incompatibility. Sfeir and coll...
3 CitationsSource
#1Flavia Fontanesi (UM: University of Miami)H-Index: 25
#2Marco Tigano (NYU: New York University)H-Index: 6
Last. Antoni Barrientos (UM: University of Miami)H-Index: 54
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Abstract The maintenance and expression of the mammalian mitochondrial genome (mtDNA) is a fundamental aspect of mitochondrial biogenesis. The mtDNA is transcribed as precursor polycistronic transcripts containing 11 mRNAs, 2 rRNAs, punctuated by 22 tRNAs. They require to be processed to engage in the assembly of unique mitochondrial ribosomes (the rRNAs), or mRNA translation into 13 proteins, all components of the OXPHOS enzymes, essential for aerobic cellular energy production. Through evoluti...
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#1Marco Tigano (NYU: New York University)H-Index: 6
#2Aaron Fraser Phillips (NYU: New York University)
Last. Agnel Sfeir (NYU: New York University)H-Index: 23
view all 3 authors...
Abstract DNA combing technology is a powerful methodology for the study of DNA replication in vivo. This tool can be used to identify origins of replication, assess of directionality of forks, and measure fork speed. Over the years, the method has been used extensively to study nuclear DNA replication. The first step involves the incorporation of thymidine analogs (CldU and IdU) into nascent DNA chains and followed by their visualization with immunofluorescence using antibodies that can distingu...
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#1Adrienne A. Phillips (NYU: New York University)H-Index: 15
#2Armêl R. Millet (French Institute of Health and Medical Research)H-Index: 1
Last. Agnel Sfeir (NYU: New York University)H-Index: 23
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Summary Mutations in mtDNA lead to muscular and neurological diseases and are linked to aging. The most frequent aberrancy is the "common deletion" that involves a 4,977-bp region flanked by 13-bp repeats. To investigate the basis of this deletion, we developed a single-molecule mtDNA combing method. The analysis of replicating mtDNA molecules provided in vivo evidence in support of the asymmetric mode of replication. Furthermore, we observed frequent fork stalling at the junction of the common ...
64 CitationsSource