Olivier Zelphati
University of California, San Francisco
Genetic enhancementTransduction (genetics)BiophysicsOligonucleotideGene deliveryCellMolecular biologyCationic liposomeMagnetofectionCationic polymerizationLiposomeChemistryIn vitroIn vivoSmall interfering RNANucleic acidRetinaDrug deliveryEndocytosisTransfectionDNABiochemistryRNAMedicineBiologyMagnetic nanoparticlesCell biology
27Publications
14H-index
2,091Citations
Publications 26
Newest
#1Marco BassettoH-Index: 1
#2Merve Sen (University of Tübingen)H-Index: 2
Last. Olivier ZelphatiH-Index: 14
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The prevalence of retinal disorders associated with visual impairment and blindness is increasing worldwide, while most of them remain without effective treatment. Pharmacological and molecular therapy development is hampered by the lack of effective drug delivery into the posterior segment of the eye. Among molecular approaches, RNA-interference (RNAi) features strong advantages, yet delivering it to the inner layer of the retina appears extremely challenging. To address this, we developed an o...
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#1Merve Sen (University of Tübingen)H-Index: 2
#2Marco BassettoH-Index: 1
Last. Blanca Arango-Gonzalez (University of Tübingen)H-Index: 18
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The use of synthetic RNA for research purposes as well as RNA-based therapy and vaccination has gained increasing importance. Given the anatomical seclusion of the eye, small interfering RNA (siRNA)-induced gene silencing bears great potential for targeted reduction of pathological gene expression that may allow rational treatment of chronic eye diseases in the future. However, there is yet an unmet need for techniques providing safe and efficient siRNA delivery to the retina. We used magnetic n...
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#1Marco BassettoH-Index: 1
#2Daniel Roberto Ajoy Moreno (UDS: University of Strasbourg)
Last. Olivier ZelphatiH-Index: 14
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#1Daniel Roberto Ajoy Moreno (UDS: University of Strasbourg)
Last. Vincent Marion (UDS: University of Strasbourg)H-Index: 19
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#1C. Di Scala (AMU: Aix-Marseille University)
#2M. Tessier (AMU: Aix-Marseille University)
Last. Christophe Pellegrino (AMU: Aix-Marseille University)H-Index: 21
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Abstract Background Gene delivery within the central nervous system at postnatal age is one of the most challenging tasks in neuroscience and currently only a few effective methods are available. Comparison with existing methods For postnatal central nervous system cells, viral approaches are commonly used for genetic engineering but they face several biosafety requirements for production and use making them less accessible to the community. Conversely, lipid-based methods are widely used in cel...
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Advances in RNA technology during the past two decades have led to the construction of replication-competent RNA, termed replicons, RepRNA, or self-amplifying mRNA, with high potential for vaccine applications. Cytosolic delivery is essential for their translation and self-replication, without infectious progeny generation, providing high levels of antigen expression for inducing humoral and cellular immunity. Synthetic nanoparticle-based delivery vehicles can both protect the RNA molecules and ...
17 CitationsSource
#1Silke Smolders (Katholieke Universiteit Leuven)H-Index: 4
#2Sofie KesselsH-Index: 4
Last. Bert BrôneH-Index: 17
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We thank Petra Bex for helping with all experiments. Financial support for this research was granted by the UHasselt (BOF13N01, BOF16NI04), the Research Foundation of Flanders 7 (FWO G0A0513) and Rotary campaign “Hope in Head”.
10 CitationsSource
#1Sofie KesselsH-Index: 4
#2Silke SmoldersH-Index: 4
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We thank Petra Bex for helping with all experiments. Financial support for this research was granted by the UHasselt (BOF13N01, BOF16N104), the Research Foundation of Flanders 7 (FWO G0A0513) and Rotary campaign "Hope in Head".
#1Cédric SapetH-Index: 8
#2Nicolas LaurentH-Index: 12
Last. Christophe BeclinH-Index: 14
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Protocol Summary Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well as for high-throughput projects. Our method yields high transfection efficiency and can be used for deciphering the genetic control of neural cell differentiation.
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#1Cédric SapetH-Index: 8
#2Cécile FormosaH-Index: 1
Last. Nicolas LaurentH-Index: 12
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Background: 3D matrices are widely used as cell growth supports in basic research, regenerative medicine or cell-based drug assays. In order to genetically manipulate cells cultured within 3D matrices, two novel non-viral transfection reagents allowing preparation of matrices for in situ cell transfection were evaluated. Results: Two lipidic formulations, 3D-Fect™ and 3D-FectIN™, were assessed for their ability to transfect cells cultured within 3D solid scaffolds and 3D hydrogels, respectively....
7 CitationsSource