Marie-Luise Lippert
University of Marburg
ATP synthaseInorganic chemistryEnzymeChemistryElectron paramagnetic resonanceBiocatalysisCofactorBenzylsuccinate synthaseMethyl groupDouble bondStereochemistry
Publications 2
#1Silke von Horsten (University of Marburg)H-Index: 4
#2Marie-Luise Lippert (University of Marburg)H-Index: 1
Last. Johann Heider (University of Marburg)H-Index: 54
view all 7 authors...
Anaerobic toluene degradation proceeds by fumarate addition to produce (R)-benzylsuccinate as first intermediate, which is further degraded via β-oxidation by five enzymes encoded in the conserved bbs operon. This study characterizes two enzymes of this pathway, (E)-benzylidenesuccinyl-CoA hydratase (BbsH), and (S,R)-2-(α-hydroxybenzyl)succinyl-CoA dehydrogenase (BbsCD) from Thauera aromatica. BbsH, a member of the enoyl-CoA hydratase family, converts (E)-benzylidenesuccinyl-CoA to 2-(α-hydroxyb...
#1Markus Hilberg (University of Marburg)H-Index: 3
#2Antonio J. Pierik (University of Marburg)H-Index: 63
Last. Johann Heider (University of Marburg)H-Index: 54
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The anaerobic degradation pathway of toluene is initiated by the addition of the methyl group of toluene to the double bond of fumarate. This reaction is catalyzed by a novel glycyl-radical enzyme, (R)-benzylsuccinate synthase (BSS). The enzyme consists of three subunits, α, β, and γ, and differs from most other glycyl-radical enzymes in having additional cofactors. We have purified a Strep-tagged nonactivated BSS from recombinant Escherichia coli and identified the additional cofactors as FeS c...
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