Kimberly A. Mace
University of Manchester
HaematopoiesisHox geneHomeoboxEndothelial stem cellCellular differentiationRegeneration (biology)Progenitor cellImmunologyInflammationMacrophageMyeloidBone marrowWound healingAngiogenesisStem cellCancer researchMedicineTranscription factorBiologyCell biology
Publications 39
#1Laure Bridoux (University of Manchester)H-Index: 2
#2Peyman Zarrineh (RMIT: RMIT University)H-Index: 1
Last. Nicoletta Bobola (University of Manchester)H-Index: 19
view all 12 authors...
Gene expression programs determine cell fate in embryonic development and their dysregulation results in disease. Transcription factors (TFs) control gene expression by binding to enhancers, but how TFs select and activate their target enhancers is still unclear. HOX TFs share conserved homeodomains with highly similar sequence recognition properties, yet they impart the identity of different animal body parts. To understand how HOX TFs control their specific transcriptional programs in vivo, we...
2 CitationsSource
#1J. GherardiniH-Index: 3
#2Youhei Uchida (Kadai: Kagoshima University)H-Index: 1
Last. Ralf PausH-Index: 127
view all 7 authors...
Besides monocyte (MO)-derived macrophages (MACs), self-renewing tissue-resident macrophages (trMACs) maintain the intracutaneous MAC pool in murine skin. Here, we have asked whether the same phenomenon occurs in human skin using organ-cultured, full-thickness skin detached from blood circulation and bone marrow. Skin stimulation ex vivo with the neuropeptide substance P (SP), mimicking neurogenic skin inflammation, significantly increased the number of CD68+MACs in the papillary dermis without a...
2 CitationsSource
#1Holly N. Wilkinson (Hull York Medical School)H-Index: 8
#2Sophie E. Upson (Hull York Medical School)H-Index: 1
Last. Matthew J. Hardman (Hull York Medical School)H-Index: 34
view all 6 authors...
Iron is crucial for maintaining normal bodily function with well-documented roles in erythropoiesis, hemostasis, and inflammation. Despite this, little is known about the temporal regulation of iron during wound healing, or how iron contributes to wound biology and pathology. In this study, we profiled tissue iron levels across a healing time-course, identifying iron accumulation during late-stage repair. Diabetic murine wounds displayed significantly reduced iron levels, delayed extracellular m...
6 CitationsSource
#1Salma Alrdahe (MAHSC: Manchester Academic Health Science Centre)H-Index: 1
#2Hadeel Al Sadoun (KAU: King Abdulaziz University)H-Index: 3
Last. Kimberly A. Mace (MAHSC: Manchester Academic Health Science Centre)H-Index: 16
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: Controlled inflammatory responses of myeloid cells recruited to wounds are essential for effective repair. In diabetes, the inflammatory response is prolonged and augmented over time, with increased myeloid cells present in the wound that fail to switch from a pro-inflammatory phenotype to a pro-healing phenotype. These defects lead to delayed angiogenesis and tissue repair and regeneration, and contribute to chronic wound formation. In mouse models of diabetes, this aberrant phenotype is part...
1 CitationsSource
#1Holly N. Wilkinson (Hull York Medical School)H-Index: 8
#2Elizabeth Rose Roberts (Hull York Medical School)H-Index: 1
Last. Matthew J. Hardman (Hull York Medical School)H-Index: 34
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Abstract Macrophages are important for effective iron recycling and erythropoiesis, but also play a crucial role in wound healing, orchestrating tissue repair. Recently, we demonstrated a significant accumulation of iron in healing wounds, and a requirement of iron for effective repair. Here, we sought to determine the influence of iron on macrophage function in the context of wound healing. Interestingly, wound macrophages extensively sequestered iron throughout healing, associated with a pro-h...
12 CitationsSource
#1Tanja Torbica (University of Manchester)H-Index: 3
#2Kate Wicks (University of Manchester)H-Index: 9
Last. Kimberly A. Mace (University of Manchester)H-Index: 16
view all 8 authors...
Chronic inflammation is a hallmark of impaired healing in a plethora of tissues, including skin, and is associated with aging and diseases such as diabetes. Diabetic chronic skin wounds are characterized by excessive myeloid cells that display an aberrant phenotype, partially mediated by stable intrinsic changes induced during hematopoietic development. However, the relative contribution of myeloid cell–intrinsic factors to chronic inflammation versus aberrant signals from the local environmenta...
3 CitationsSource
#1Matthew Burgess (University of Manchester)H-Index: 3
#2Kate Wicks (University of Manchester)H-Index: 9
Last. Kimberly A. Mace (University of Manchester)H-Index: 16
view all 4 authors...
Bone marrow (BM)–derived classical monocytes are critical to wound repair, where they differentiate into macrophages and purge foreign materials and dead cells while also laying the framework for tissue repair and regeneration. A subset of this recruited population persists in the wound and acquires alternative activation states to promote cell proliferation and matrix remodeling. In diabetes, this phenotypic switch is impaired and inflammation persists in an elevated state, contributing to dela...
5 CitationsSource
#1Holly N. Wilkinson (Hull York Medical School)H-Index: 8
#2Christopher Clowes (Royal Stoke University Hospital)H-Index: 1
Last. Matthew J. Hardman (Hull York Medical School)H-Index: 34
view all 6 authors...
Cellular senescence can be broadly defined as a stable, but essentially irreversible, loss of proliferative capacity. Historically, senescence has been described as a negative outcome of advanced cellular age. It is now clear, however, that senescence represents a dynamic autonomous stress response, integral to long-term tumor suppression. Transient induction of a senescent phenotype has actually been suggested to promote regeneration in both liver and skin. Here, we explored the role of senesce...
17 CitationsSource
#1Takahiro Umehara (Nagasaki University)H-Index: 8
#2Ryoichi Mori (Nagasaki University)H-Index: 15
Last. Kazuya Ikematsu (Nagasaki University)H-Index: 14
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Neutrophils are involved in the first stage of acute inflammation. Following injury, they are mobilized and recruited to the injured tissue. In diabetes, wound healing is delayed and aberrant, leading to excessive recruitment and retention of neutrophils that fail to promote angiogenesis and prolong inflammation. However, the exact pathological mechanisms of diabetic-derived neutrophils in chronic inflammation remain unclear. Here, microRNA (miRNA) profiling of neutrophils from bone marrow in ty...
14 CitationsSource
#1Dina S. Vara (University of Exeter)H-Index: 12
#2Joanna M Watt (University of Bath)H-Index: 6
Last. Giordano Pula (University of Exeter)H-Index: 22
view all 11 authors...
Abstract Aims: Deoxyribose-1-phosphate (dRP) is a proangiogenic paracrine stimulus released by cancer cells, platelets, and macrophages and acting on endothelial cells. The objective of this study was to clarify how dRP stimulates angiogenic responses in human endothelial cells. Results: Live cell imaging, electron paramagnetic resonance, pull-down of dRP-interacting proteins, followed by immunoblotting, gene silencing of different NADPH oxidases (NOXs), and their regulatory cosubunits by small ...
16 CitationsSource