Silke von Horsten
University of Marburg
Hydrogen bondBiophysicsIsomerizationImportinCryptochromeKineticsPhotolyaseHydrogen–deuterium exchangeChemistryNLSChromophoreChlamydomonas reinhardtiiStructural communicationBifunctionalNucleusResonance Raman spectroscopyPhytochrome APhytochrome BLight controlActin cytoskeletonLight drivenPhototropinDNA binding siteNuclear localization sequenceDNADouble bondFar-redStereochemistryFlavoproteinBiologyPhytochromeCell biology
Publications 6
#1Silke von Horsten (University of Marburg)H-Index: 4
#2Marie-Luise Lippert (University of Marburg)H-Index: 1
Last. Johann Heider (University of Marburg)H-Index: 54
view all 7 authors...
Anaerobic toluene degradation proceeds by fumarate addition to produce (R)-benzylsuccinate as first intermediate, which is further degraded via β-oxidation by five enzymes encoded in the conserved bbs operon. This study characterizes two enzymes of this pathway, (E)-benzylidenesuccinyl-CoA hydratase (BbsH), and (S,R)-2-(α-hydroxybenzyl)succinyl-CoA dehydrogenase (BbsCD) from Thauera aromatica. BbsH, a member of the enoyl-CoA hydratase family, converts (E)-benzylidenesuccinyl-CoA to 2-(α-hydroxyb...
Phytochrome activity is not only controlled by light but also by post-translational modifications, e. g. phosphorylation. One of the phosphatases responsible for plant phytochrome dephospho-rylation and thereby increased activity is the phytochrome-associated protein phosphatase 5 (PAPP5). We show that PAPP5 recognizes phospho-site mimicking mutants of phytochrome B, when being activated by arachidonic acid (AA). Addition of AA to PAPP5 decreases the -helical content as tracked by CD-spectrosco...
#1Sophie Franz-BadurH-Index: 2
#2Alexander PennerH-Index: 1
Last. Lars-Oliver EssenH-Index: 54
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Cryptochromes (CRYs) are an ubiquitously occurring class of photoreceptors, which are important for regulating the circadian rhythm of animals via a time-delayed transcription-translation feedback loop (TTFL). Due to their protein architecture and common FAD chromophore, they belong to the same superfamily as photolyases (PHLs), an enzyme class that repairs UV-induced DNA lesions upon blue light absorption. Apart from their different functions the only prominent structural difference between CRY...
#1Hanna HelizonH-Index: 2
#2Jutta Rösler-Dalton (University of California, Berkeley)H-Index: 1
Last. Mathias ZeidlerH-Index: 13
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: Phytochrome A (phyA) is a red and far-red (FR) sensing photoreceptor regulating plant growth and development. Its biologically active FR-absorbing form Pfr translocates into the nucleus and subsequently regulates gene expression. Two transport facilitators, FR elongated hypocotyl 1 (FHY1) and FHY1-like (FHL), are crucial for its cytoplasmic-nuclear translocation. FHY1 interacts preferentially with activated phyA (Pfr) in assays with recombinant phyA and FHY1 and in vivo. Nuclear translocation ...
#1Francisco Velazquez Escobar (Technical University of Berlin)H-Index: 15
#2David Buhrke (Technical University of Berlin)H-Index: 10
Last. Peter Hildebrandt (Technical University of Berlin)H-Index: 75
view all 8 authors...
The N-terminal extension (NTE) of plant phytochromes has been suggested to play a functional role in signaling photoinduced structural changes. Here, we use resonance Raman spectroscopy to study the effect of the NTE on the chromophore structure of B-type phytochromes from two evolutionarily distant plants. NTE deletion seems to have no effect on the chromophore in the inactive Pr state, but alters the torsion of the C-D ring methine bridge and the surrounding hydrogen bonding network in the phy...
#1Silke von HorstenH-Index: 4
#2Simon StraßH-Index: 2
Last. Lars-Oliver Essen (University of Marburg)H-Index: 54
view all 9 authors...
Mapping light-driven conformational changes within the photosensory module of plant phytochrome B
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