Pauline M. Rudd
BlackRock
GlycomicsGlycoproteinInternal medicineMolecular biologyGlycomeAntibodyMass spectrometryChemistryGlycobiologyGlycanFucoseGlycosylationFucosylationSialic acidN-linked glycosylationComputer scienceChromatographyCancer researchMonoclonal antibodyBiochemistryComputational biologyMedicineBiology
95Publications
28H-index
2,308Citations
Publications 96
Newest
#1Hayden Wilkinson (UCD: University College Dublin)H-Index: 1
#2Kristina A. Thomsson (University of Gothenburg)H-Index: 20
Last. Radka Saldova (UCD: University College Dublin)H-Index: 29
view all 0 authors...
O-Glycosylation changes in misfolded proteins are of particular interest in understanding neurodegenerative conditions such as Parkinson's disease (PD) and incidental Lewy body disease (ILBD). This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approac...
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#1Gordon Greville (BlackRock)H-Index: 3
#2Esther Llop (University of Girona)H-Index: 10
Last. Radka Saldova (BlackRock)H-Index: 29
view all 9 authors...
BACKGROUND Glycosylation, one of the most fundamental post-translational modifications, is altered in cancer and is subject in part, to epigenetic regulation. As there are many epigenetic-targeted therapies currently in clinical trials for the treatment of a variety of cancers, it is important to understand the impact epi-therapeutics have on glycosylation. RESULTS Ovarian and triple negative breast cancer cells were treated with the DNA methyltransferase inhibitor, 5-AZA-2-deoxycytidine (5-AZA-...
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#1Sarah GilgunnH-Index: 6
#2Keefe Murphy (MU: Maynooth University)
Last. Radka SaldovaH-Index: 29
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The diagnosis and treatment of prostate cancer (PCa) is a major health-care concern worldwide. This cancer can manifest itself in many distinct forms and the transition from clinically indolent PCa to the more invasive aggressive form remains poorly understood. It is now universally accepted that glycan expression patterns change with the cellular modifications that accompany the onset of tumorigenesis. The aim of this study was to investigate if differential glycosylation patterns could disting...
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#1Edward Pallister (University of Manchester)H-Index: 4
#2Matthew S. F. Choo (Agency for Science, Technology and Research)H-Index: 3
Last. Terry Nguyen-Khuong (Agency for Science, Technology and Research)H-Index: 10
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High-throughput glycan analysis has become an important part of biopharmaceutical production and quality control. However, it is still a significant challenge in the field of glycomics to easily deduce isomeric glycan structures, especially in a high-throughput manner. Ion mobility spectrometry (IMS) is an excellent tool for differentiating isomeric glycan structures. However, demonstrations of the utility of IMS in high-throughput workflows such as liquid chromatography-fluorescence-mass spectr...
1 CitationsSource
#1Edward Pallister (University of Manchester)H-Index: 4
#2Matthew S. F. Choo (Agency for Science, Technology and Research)H-Index: 3
Last. Terry Nguyen-Khuong (Agency for Science, Technology and Research)H-Index: 10
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Sialic acids are sugars present in many animal glycoproteins and are of particular interest in biopharmaceuticals, where a lack of sialylation can reduce bioactivity. Here, we describe how α-2,6-sialyltransferase from Photobacterium damselae can be used to markedly increase the level of sialylation of CHO-produced α-1-antitrypsin. Detailed analysis of the sialylation products showed that in addition to the expected α-2,6-sialylation of galactose, a second disialyl galactose motif Neu5Ac-α2,3(Neu...
4 CitationsSource
#1Ian WalshH-Index: 21
#2Matthew S. F. Choo (Agency for Science, Technology and Research)H-Index: 3
Last. Terry Nguyen-KhuongH-Index: 10
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The accurate assessment of antibody glycosylation during bioprocessing requires the high-throughput generation of large amounts of glycomics data. This allows bioprocess engineers to identify critical process parameters that control the glycosylation critical quality attributes. The advances made in protocols for capillary electrophoresis-laser-induced fluorescence (CE-LIF) measurements of antibody N-glycans have increased the potential for generating large datasets of N-glycosylation values for...
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#1Juhi Samal (NUI: National University of Ireland)H-Index: 6
#1Juhi Samal (NUI: National University of Ireland)H-Index: 1
Last. Roisin O’FlahertyH-Index: 9
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N--glycan alterations in the nervous system can result in different neuropathological symptoms like mental retardation, seizures and epilepsy. Studies have reported the characterization of N-glycan...
1 CitationsSource
#1Gordon Greville (UCD: University College Dublin)H-Index: 3
#1Gordon Greville (UCD: University College Dublin)
Last. Radka SaldovaH-Index: 29
view all 11 authors...
Background: Glycosylation is one of the most fundamental post-translational modifications. Importantly, glycosylation is altered in many cancers. These alterations have been proven to impact on tumor progression and to promote tumor cell survival. From the literature, it is known that there is a clear link between chemoresistance and hypoxia, hypoxia and epigenetics and more recently glycosylation and epigenetics. Methods and Results: Our objective was to investigate these differential parameter...
2 CitationsSource
#1Hao D. Cheng (Dartmouth College)H-Index: 6
#2Irit Tirosh (Boston Children's Hospital)H-Index: 9
Last. Peter A. Nigrovic (Brigham and Women's Hospital)H-Index: 50
view all 17 authors...
IgG Fc glycans were characterized in 225 healthy children and 40 children with unexplained recurrent respiratory infections, revealing age-dependent variation and suggesting a role for IgG glycosylation in immunocompetence.
5 CitationsSource
#1Belinda Kumpel (NHSBT: NHS Blood and Transplant)H-Index: 13
#2Radka SaldovaH-Index: 29
Last. Manfred Wuhrer (LUMC: Leiden University Medical Center)H-Index: 83
view all 11 authors...
Anti-D immunoglobulin (Anti-D Ig) prophylaxis prevents haemolytic disease of the fetus and newborn. Monoclonal IgG anti-Ds (mAb-Ds) would enable unlimited supplies but have differed in efficacy in FcγRIIIa-mediated ADCC assays and clinical trials. Structural variations of the oligosaccharide chains of mAb-Ds are hypothesised to be responsible. Quantitative data on 12 Fc-glycosylation features of 23 mAb-Ds (12 clones, 5 produced from multiple cell lines) and one blood donor-derived anti-D Ig were...
2 CitationsSource