Geneviève Guillemin
Paris Diderot University
AnatomyBiomedical engineeringSurgeryPathologyCoralResorptionMaterials scienceMesenchymal stem cellMetatarsal bonesBone tissueNonunionType I collagenLong boneImplantCortical boneStem cellBone regenerationBone tissue engineeringBone formationInduced membraneBone morphogenetic proteinTissue engineeringMedicineBone healingBiologyScaffold
13Publications
10H-index
2,050Citations
Publications 13
Newest
#1Véronique Viateau ('ENS Paris': École Normale Supérieure)H-Index: 14
#2Morad Bensidhoum (Paris Diderot University)H-Index: 21
Last. Philippe PélissierH-Index: 1
view all 7 authors...
Abstract Animal experiments using the induced membrane procedure for bone tissue engineering purposes have provided evidence that the membrane has structural characteristics and biologic properties that may be used for bone tissue engineering purposes. Clinically relevant animal models have demonstrated that standardized particulate bone constructs can be used to repair large bone defects using the procedure and that the osteogenic ability of these constructs partially approaches that of bone au...
77 CitationsSource
#1V. ViateauH-Index: 9
#1Véronique ViateauH-Index: 14
Last. Hervé PetiteH-Index: 32
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Purpose of the study: The objective of this study was to establish an experimental sheep model for a surgical procedure which has been clinically successful for repairing major loss of bone stock: the Masquelet technique. Material and methods: A 25 mm bone defect was created in a metatarsal bone then filled with a cement filler. After six weeks, the cement was removed after opening the neoformed pseudosynovial membrane. The cavity was left empty in group 1 (n=6) or filled with a morcelized cance...
3 Citations
#1V. Viateau (École nationale vétérinaire d'Alfort)H-Index: 9
Last. Hervé Petite (Paris Diderot University)H-Index: 32
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To assess the efficacy of engineered tissues, it is necessary to have (1) appropriate large animal models that mimic the clinical setting and (2) relevant methods of monitoring the biofuntionality of these tissues. However, developing these tissue constructs is a step-by-step process in which numerous variables such as scaffold design, source of stem cells and mode of growth factor application have to be optimized. After an in vitro optimization phase, the use of small animal models to optimize ...
18 CitationsSource
#1V. Viateau (École nationale vétérinaire d'Alfort)H-Index: 9
Last. Hervé PetiteH-Index: 32
view all 8 authors...
Standardized particulate bone constructs, obtained by expanding autologous mesenchymal stem cells (MSCs) onto coral granules in vitro, were transplanted into long-bone, critical-size defects in sheep. Control experiments were also performed in which autologous bone grafts were implanted. Defect cavities were lined with a preformed vascularized membrane (induced by temporarily inserting a cement spacer for 6 weeks prior to bone construct implantation), which served as a mold keeping the engineere...
248 CitationsSource
#1Véronique Viateau (CNRS: Centre national de la recherche scientifique)H-Index: 14
#2Geneviève Guillemin (CNRS: Centre national de la recherche scientifique)H-Index: 10
Last. Hervé Petite (CNRS: Centre national de la recherche scientifique)H-Index: 32
view all 9 authors...
Objectives— To report an ovine model that can be used to evaluate the efficacy of bone substitutes for repair of segmental diaphyseal bone defects. Study Design— Experimental study. Animals— Eleven 2-year-old Pre-Alpes Sheep. Methods— Mid-diaphyseal metatarsal bone defects (25 mm long) were stabilized by a dynamic compression plate over a polymethylmethacrylate (PMMA) cement spacer, and by external coaptation. The PMMA spacer was removed at 6 weeks by incising the encapsulating membrane. The def...
129 CitationsSource
#1Véronique Viateau ('ENS Paris': École Normale Supérieure)H-Index: 14
view all 7 authors...
Source
#1Wassila Bensaïd (CNRS: Centre national de la recherche scientifique)H-Index: 4
#2Karim OudinaH-Index: 18
Last. Hervé PetiteH-Index: 32
view all 9 authors...
Large bone defects are still a challenge to orthopedic surgeons. In this study, a massive bone defect with a clinically relevant volume was efficiently reconstructed by transplanting an engineered bone in which mesenchymal stem cells (MSCs) expanded in autologous serum (AS) were combined with a porous scaffold. In the first step, we established that the way in which the MSCs are distributed over the scaffold affects the ultimate bone-forming ability of the transplant: constructs consisting of a ...
127 CitationsSource
#1Véronique Viateau (CNRS: Centre national de la recherche scientifique)H-Index: 14
Last. Hervé PetiteH-Index: 32
view all 9 authors...
Objective—To develop a technique for use in investigation of healing of long-bone defects by creation of a critical-size defect in the left metarsal III and IV bone (metatarsus) of sheep. Animals—18 healthy adult sheep. Procedure—Sheep were allocated to 4 groups (3, 3, 5, and 7 sheep in groups 1 to 4, respectively). An ostectomy with various segmental length-to-diaphyseal diameter ratios (0.5, 1.0, 2.0, and 2.0 for groups 1 to 4, respectively) was performed on the left metatarsus of each sheep. ...
27 CitationsSource
#1Hervé Petite (CNRS: Centre national de la recherche scientifique)H-Index: 32
#2Véronique Viateau (CNRS: Centre national de la recherche scientifique)H-Index: 14
Last. Geneviève Guillemin (CNRS: Centre national de la recherche scientifique)H-Index: 10
view all 9 authors...
Bone lesions above a critical size become scarred rather than regenerated, leading to nonunion. We have attempted to obtain a greater degree of regeneration by using a resorbable scaffold with regeneration-competent cells to recreate an embryonic environment in injured adult tissues, and thus improve clinical outcome. We have used a combination of a coral scaffold with in vitro-expanded marrow stromal cells (MSC) to increase osteogenesis more than that obtained with the scaffold alone or the sca...
1,887 CitationsSource
#1Gerard CuninH-Index: 1
#2Herve BoissonnetH-Index: 1
Last. Geneviève GuilleminH-Index: 10
view all 5 authors...
STUDY DESIGN: Osteoporotic human cadaveric thoracic vertebral bodies and vertebral bodies from mature sheep were used as model systems to assess coral resorption and new bone formation after injection of coral granules. OBJECTIVE: To evaluate the use of natural coral exoskeleton, an osteoconductive material, for the filling of vertebral bodies. SUMMARY OF BACKGROUND DATA: Percutaneous injection of polymethylmetacrylate (PMMA) is often proposed for prophylactically stabilizing osteoporotic verteb...
129 CitationsSource