Soghra Khani
Pasteur Institute of Iran
Biological activityMode of actionCell wallGenotypeSingle-nucleotide polymorphismAffinity chromatographyAlleleChemistryImmunologyLactobacillus rhamnosusCyanogen bromideCell membraneAspergillusMacrophagePeptideFOXP3Aspergillus fumigatusLactobacillusProbioticCandida albicansTuberculosisHerpes simplex virusAntifungal drugRecombinant DNAPhysiologyBacteriaAntimicrobial peptidesEscherichia coliBiochemistryVirus quantificationMedicineBiologyEdman degradationImmune systemMicrobiology
15Publications
7H-index
193Citations
Publications 20
Newest
#1Sima Sadat Seyedjavadi (Pasteur Institute of Iran)H-Index: 10
#2Soghra Khani (Pasteur Institute of Iran)H-Index: 10
Last. Mehdi Razzaghi-Abyaneh (Pasteur Institute of Iran)H-Index: 24
view all 7 authors...
Due to increasing the rate of invasive fungal infections and emerging antifungal resistance, development of novel antifungal drugs has been an urgent necessity. Antifungal peptides (AFPs) have recently attracted attention due to their unique ability to evade the drug resistant fungal pathogens. In this study, a novel AFP, Cc-AFP1, with a molecular weight of ~ 3.759 kDa, was isolated from Carum carvi L., purified by ammonium sulfate precipitation and reverse phase HPLC and finally identified by s...
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#1Soghra Khani (Pasteur Institute of Iran)H-Index: 10
#2Sima Sadat Seyedjavadi (Pasteur Institute of Iran)H-Index: 10
Last. Mehdi Razzaghi-AbyanehH-Index: 24
view all 11 authors...
DiSkh-AMP1, a novel dimeric antifungal peptide contained 65 amino acid residues was recombinant produced by a flexible linker to improve the antifungal activity of native Skh-AMP1 isolated from Satureja khuzistanica leaves. To discover a new system for expressing DiSkh-AMP1 in Escherichia coli BL21, the DiSkh-AMP1 gene was synthesized and inserted into pET28a vector in which fusion peptide (3.5 kDa) was used as a fusion partner and an N-terminal 6-His as an affinity tag. The fusion/DiSkh-AMP1 pe...
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#1Soghra Khani (Pasteur Institute of Iran)H-Index: 10
#2Mina Barzegari (Pasteur Institute of Iran)H-Index: 1
Last. Ali Zekri (IUMS: Iran University of Medical Sciences)H-Index: 12
view all 13 authors...
OBJECTIVES This study aimed to evaluate the biochemical factors, genetic mutations, outcome of treatment, and clinical follow-up data of Iranian patients with tetrahydrobiopterin (BH4) deficiency from April/2016 to March/2020. METHODS Forty-seven BH4 deficiency patients were included in the study and underwent biochemical and genetic analyses. The clinical outcomes of the patients were evaluated after long-term treatment. RESULTS Out of the 47 (25 females and 22 males) BH4 deficiency patients en...
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#2Soghra KhaniH-Index: 10
#4Hadi Zare-ZardiniH-Index: 12
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#1Sima Sadat Seyedjavadi (Pasteur Institute of Iran)H-Index: 10
#2Soghra Khani (Pasteur Institute of Iran)H-Index: 10
Last. Mehdi Razzaghi-Abyaneh (Pasteur Institute of Iran)H-Index: 24
view all 10 authors...
Fungal species resistant to current antifungal agents are considered as a serious threat to human health; the dilemma that has dragged attentions toward other sources of antifungals such as antimicrobial peptides (AMPs). In order to improve biological activity of a recently described antifungal peptide MCh-AMP1 from Matricaria chamomilla flowers, MCh-AMP1dimer (DiMCh-AMP1), containing 61 amino acid residues connected by flexible linker (GPDGSGPDESGPDES) was designed, expressed in Escherichia col...
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#1Sima Sadat Seyedjavadi (Pasteur Institute of Iran)H-Index: 10
#2Soghra Khani (Pasteur Institute of Iran)H-Index: 10
Last. Mehdi Razzaghi-Abyaneh (Pasteur Institute of Iran)H-Index: 24
view all 11 authors...
The rise of antifungal drug resistance in Candida species responsible for life threatening candidiasis is considered as an increasing challenge for the public health. MCh-AMP1 has previously been reported as a natural peptide from Matricaria chamomilla L. flowers with broad-spectrum antifungal activity against human pathogenic moulds and yeasts. In the current study, the mode of action of synthetic MCh-AMP1 was investigated against Candida albicans, the major etiologic agent of life-threatening ...
13 CitationsSource
#1Soghra Khani (Pasteur Institute of Iran)H-Index: 10
#2Sima Sadat Seyedjavadi (Pasteur Institute of Iran)H-Index: 10
Last. Mehdi Razzaghi-Abyaneh (Pasteur Institute of Iran)H-Index: 24
view all 11 authors...
Abstract Skh-AMP1 (GRTSKQELCTWERGSVRQADKTIAG) is an antifungal peptide isolated from Satureja khuzistanica which has been shown to have strong antifungal activity against Aspergillus and Candida species, but no obvious hemolytic effects or cell cytotoxicity in vitro. In the present study, Skh-AMP1 was synthesized, and its mode of action on the plasma membrane, mitochondria, and morphological and ultrastructural changes against conidia and hyphae of Aspergillus fumigatus were evaluated. The resul...
5 CitationsSource
#1Soghra Khani (Pasteur Institute of Iran)H-Index: 10
#2Sima Sadat Seyedjavadi (Pasteur Institute of Iran)H-Index: 10
Last. Mehdi Razzaghi-Abyaneh (Pasteur Institute of Iran)H-Index: 24
view all 9 authors...
Abstract The increasing resistance of pathogenic fungi to conventional antifungal therapies is a major global health concern. Currently, antifungal peptides are receiving increasing attention as suitable candidates for antifungal drug discovery. In the present study, an antifungal peptide was isolated from Satureja khuzistanica by reverse phase-HPLC column and sequenced by de novo sequencing and Edman degradation. The peptide cytotoxicity on human red blood cells and HEK293 cells was assessed us...
7 CitationsSource
#1Sima Sadat Seyedjavadi (Pasteur Institute of Iran)H-Index: 10
#2Soghra Khani (Pasteur Institute of Iran)H-Index: 10
Last. Mehdi Razzaghi-Abyaneh (Pasteur Institute of Iran)H-Index: 24
view all 9 authors...
: The antimicrobial activities of natural products have attracted much attention due to the increasing incidence of pathogens that have become resistant to drugs. Thus, it has been attempted to promisingly manage infectious diseases via a new group of therapeutic agents called antimicrobial peptides. In this study, a novel antifungal peptide, MCh-AMP1, was purified by reverse phase HPLC and sequenced by de novo sequencing and Edman degradation. The antifungal activity, safety, thermal, and pH st...
12 CitationsSource
#1Elham Beiranvand (Mazandaran University of Medical Sciences)H-Index: 3
#2Saeid Abediankenari (Mazandaran University of Medical Sciences)H-Index: 14
Last. Sima Sadat Seyedjavadi (Pasteur Institute of Iran)H-Index: 10
view all 8 authors...
Forkhead box protein 3 (FoxP3) is an important factor for development and function of Regulatory T cells (Treg). Studies have found an association between common gene polymorphisms in FoxP3 and some infectious diseases. The aim of this study was to evaluate possible associations between two Single nucleotide polymorphisms (SNPs) in the promoter of the FoxP3 gene to susceptibility to tuberculosis (TB) and the alteration of Foxp3 gene expression. The pattern distribution of genotype at two positio...
2 CitationsSource