Fernando A. Fierro
University of California, Davis
HaematopoiesisStem cell transplantation for articular cartilage repairPathologyCellular differentiationMolecular biologyStromal cellChemistryMesenchymal stem cellProgenitor cellImmunologyVascular endothelial growth factorBone marrowWound healingAngiogenesisStem cellTransplantationCancer researchMedicineBiologyCell biology
55Publications
21H-index
1,374Citations
Publications 55
Newest
#1Amanda K. Hodson (UC Davis: University of California, Davis)H-Index: 10
#2Andrew C. Cicchetto (UC Davis: University of California, Davis)H-Index: 2
Last. Fernando A. Fierro (UC Davis: University of California, Davis)H-Index: 21
view all 3 authors...
Abstract Economically damaging populations of lesion nematode and ring nematode are managed in tree crops largely through pre-plant chemical fumigation, the use of which is increasingly restricted due to human health and environmental concerns. Reducing the use of fumigants requires precise knowledge of pest nematodes’ density and distribution, however; extensive sampling is costly due to the time intensive process of nematode counting and identification. In this study, species specific primers ...
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#1Hsin-ya Yang (UC Davis: University of California, Davis)H-Index: 2
#2Fernando A. Fierro (UC Davis: University of California, Davis)H-Index: 21
Last. Roslyn Rivkah Isseroff (UC Davis: University of California, Davis)H-Index: 58
view all 16 authors...
Diabetic foot ulcers are a major health care concern with limited effective therapies. Mesenchymal stem cell (MSC)-based therapies are promising treatment options due to their beneficial effects of immunomodulation, angiogenesis, and other paracrine effects. We investigated whether a bioengineered scaffold device containing hypoxia-preconditioned, allogeneic human MSCs combined with the beta-adrenergic antagonist timolol could improve impaired wound healing in diabetic mice. Different iterations...
5 CitationsSource
#1Vivian Alonso-Garcia (UFU: Federal University of Uberlandia)H-Index: 1
#2Cutter Chaboya (UC Davis: University of California, Davis)H-Index: 1
Last. Fernando A. Fierro (UC Davis: University of California, Davis)H-Index: 21
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For hundreds of indications, mesenchymal stromal cells (MSCs) have not achieved the expected therapeutic efficacy due to an inability of the cells to reach target tissues. We show that inducing high mannose N-glycans either chemically, using the mannosidase I inhibitor Kifunensine, or genetically, using an shRNA to silence the expression of mannosidase I A1 (MAN1A1), strongly increases the motility of MSCs. We show that treatment of MSCs with Kifunensine increases cell migration toward bone frac...
28 CitationsSource
#1Dayoung Park (UC Davis: University of California, Davis)H-Index: 12
#1Diane Dayoung Park (UC Davis: University of California, Davis)H-Index: 1
Last. Carlito B. Lebrilla (UC Davis: University of California, Davis)H-Index: 92
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Membrane-bound oligosaccharides form the interfacial boundary between the cell and its environment, mediating processes such as adhesion and signaling. These structures can undergo dynamic changes in composition and expression based on cell type, external stimuli, and genetic factors. Glycosylation, therefore, is a promising target of therapeutic interventions for presently incurable forms of advanced cancer. Here, we show that cholangiocarcinoma metastasis is characterized by down-regulation of...
11 CitationsSource
#1Ashley L. Ramsay (UC Davis: University of California, Davis)H-Index: 1
#2Vivian Alonso-Garcia (UC Davis: University of California, Davis)H-Index: 1
Last. Fernando A. Fierro (UC Davis: University of California, Davis)H-Index: 21
view all 8 authors...
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#1Ashley L. Ramsay (UC Davis: University of California, Davis)H-Index: 1
#2Vivian Alonso-Garcia (UC Davis: University of California, Davis)H-Index: 1
Last. Fernando A. Fierro (UC Davis: University of California, Davis)H-Index: 21
view all 8 authors...
Patients with Snyder-Robinson Syndrome (SRS) exhibit deficient Spermidine Synthase (SMS) gene expression, which causes neurodevelopmental defects and osteoporosis, often leading to extremely fragile bones. To determine the underlying mechanism for impaired bone formation, we modelled the disease by silencing SMS in human bone marrow - derived multipotent stromal cells (MSCs) derived from healthy donors. We found that silencing SMS in MSCs led to reduced cell proliferation and deficient bone form...
1 CitationsSource
#1Alberto PaneroH-Index: 4
#2Alan M. HiraharaH-Index: 5
Last. Fernando A. Fierro (UC Davis: University of California, Davis)H-Index: 21
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Background:In vivo amniotic fluid is known to contain a population of mesenchymal stem cells (MSCs) and growth factors and has been shown to assist in healing when used as an adjunct in procedures ...
5 CitationsSource
#1Fernando A. Fierro (UC Davis: University of California, Davis)H-Index: 21
#2Nataly L. Magner (UC Davis: University of California, Davis)H-Index: 5
Last. Jan A. Nolta (UC Davis: University of California, Davis)H-Index: 66
view all 14 authors...
: Mesenchymal stem/stromal cells (MSCs) may be able to improve ischemic conditions as they can actively seek out areas of low oxygen and secrete proangiogenic factors. In more severe trauma and chronic cases, however, cells alone may not be enough. Therefore, we have combined the stem cell and angiogenic factor approaches to make a more potent therapy. We developed an engineered stem cell therapy product designed to treat critical limb ischemia that could also be used in trauma-induced scarring ...
8 CitationsSource
#1Baarkullah Awan (UC Davis: University of California, Davis)H-Index: 1
#2David Turkov (UC Davis: University of California, Davis)H-Index: 1
Last. Fernando A. Fierro (UC Davis: University of California, Davis)H-Index: 21
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Summary Since hundreds of clinical trials are investigating the use of multipotent stromal cells (MSCs) for therapeutic purposes, effective delivery of the cells to target tissues is critical. We have found an unexplored mechanism, by which basic fibroblast growth factor (FGF2) induces expression of fucosyltransferase 8 (FUT8) to increase core fucosylations of N-linked glycans of membrane-associated proteins, including several integrin subunits. Gain- and loss-of-function experiments show that F...
12 CitationsSource
#1Edith P. Karuna (UC Davis: University of California, Davis)H-Index: 4
#2Shannon S Choi (UC Davis: University of California, Davis)H-Index: 6
Last. Hsin-Yi Henry Ho (UC Davis: University of California, Davis)H-Index: 20
view all 7 authors...
Noncanonical WNT pathways function independently of the β-catenin transcriptional co-activator to regulate diverse morphogenetic and pathogenic processes. Recent studies showed that noncanonical WNTs, such as WNT5A, can signal the degradation of several downstream effectors, thereby modulating these effectors’ cellular activities. The protein domain(s) that mediates the WNT5A-dependent degradation response, however, has not been identified. By coupling protein mutagenesis experiments with a flow...
5 CitationsSource