Frank J. Carr
Merck KGaA
Fragment crystallizable regionPeripheral blood mononuclear cellNeutralizationImmunoglobulin EAntigenMolecular biologyCytotoxic T cellVirologyAntibodyMonoclonalAvidityFramework regionEpitopeIn vitroImmunologyIn vivoVirusT cellHepatitis C virusHerpesviridaeAntagonistHumanized antibodyCongenital cytomegalovirus infectionBetaherpesvirinaeImmunotherapyCD80CytokineELISPOTHuman cytomegalovirusParamyxoviridaeImmunogenicityEpitope mappingHepacivirusImmunoglobulin Fc FragmentsDeimmunizationImmunoglobulin light chainRecombinant DNATransfectionMonoclonal antibodyPotencyComplementarity determining regionBiologyCompetitive antagonistImmune system
Publications 7
#1Frank J. CarrH-Index: 6
#2Matthew BakerH-Index: 18
1 CitationsSource
#1Tim JonesH-Index: 8
Last. Matthew BakerH-Index: 18
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Abstract Immunogenicity is a major limitation to therapy with certain monoclonal antibodies and proteins. A major driver for immunogenicity is the presence of human T-cell epitopes within the protein sequence which can activate helper T-cells resulting in the sustained production of antibodies and neutralization of the therapeutic effect. Deimmunization is a new technology for location and removal of T-cell epitopes through the combined use of immunological and molecular biology techniques. In t...
51 CitationsSource
Interferon-α (IFN-α), in conjunction with ribavirin, is the current standard for the treatment of chronic hepatitis C virus (HCV) infection. This treatment requires frequent dosing, with a significant risk of the development of anti-IFN-α neutralizing antibodies that correlates with lack of efficacy or relapse. We have developed an IFN-α linked to the Fc region of human IgG1 for improved half-life and less frequent dosing. We have also identified, using a human T cell proliferation assay, three ...
67 CitationsSource
#1Andrew M. ScottH-Index: 87
#2Detlef GeleickH-Index: 3
Last. Lloyd J. OldH-Index: 166
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The Lewis Y (Le y ) antigen is a blood group-related antigen that is expressed in a high proportion of epithelial cancers (including breast, colon, ovary, and lung cancer) and is an attractive target for monoclonal antibody-directed therapy. The murine monoclonal 3S193 (IgG3) was generated in BALB/c mice by immunization with Le y -expressing cells of the MCF-7 breast carcinoma cell-line. The murine 3S193 showed high specificity for Le y in ELISA tests with synthetic Le y and Le y -containing gly...
93 Citations
A humanized monoclonal antibody that binds to the 86-kDa glycoprotein, gpUL75 (gH), of human cytomegalovirus (CMV) has been developed. The six complementarity determining regions of the heavy and light chains of the mouse antibody HCMV16 were transferred to human antibody framework sequences and combined with human antibody constant regions to produce a complete antibody. The reshaped antibody recognized cells infected with a variety of virus strains and neutralized clinical isolates of CMV as e...
25 CitationsSource
#1Philip R. TempestH-Index: 7
#2Elena BarbantiH-Index: 3
Last. Fabrizio MarcucciH-Index: 24
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ABSTRACT We have constructed several humanized versions of a monoclonal antibody (MAb78) against human tumor necrosis factor-α (huTNF-α) retaining the complementarity-determining regions (CDR) of the original mouse MAb with or without a variable number of original framework region (FR) residues. All versions, except one, showed a loss of binding affinity and neutralizing potency of at least 10-fold compared to the original mouse MAb or its chimeric equivalent. In some cases, however, the decreas...
12 CitationsSource
#1Philip R. Tempest (Aberd.: University of Aberdeen)H-Index: 7
#2Patricia Bremner (Aberd.: University of Aberdeen)H-Index: 2
Last. William J. Harris (Aberd.: University of Aberdeen)H-Index: 25
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Reshaping a Human Monoclonal Antibody to Inhibit Human Respiratory Syncytial Virus Infection in Vivo
356 CitationsSource