Yifa Zhou
Northeast Normal University
PolysaccharideGalectinEnzymeChemistryApoptosisGlucanGinsenosideGalactoseGalactanArabinogalactanGinsengPectinLymphocyte proliferationChromatographyBiochemistryMolecular massStereochemistryProtein kinase BBiologyHydrolysisCell biologyPharmacology
152Publications
29H-index
2,668Citations
Publications 153
Newest
A method using UPLC-HRMS has been developed for a rapid, simultaneous qualitative and quantitative analysis of twenty-five ginsenosides. Chromatographic separation was achieved on a C18 analytical column with an elution gradient comprising 0.1% aqueous formate/acetonitrile as the mobile phase. HRMS detection acquired full mass data for quantification and fullms-ddms2 (i.e., data-dependent scan mode) yielded product ion spectra for identification. Furthermore, quantitative analysis of multiginsen...
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#1Siying Zhang (Northeast Normal University)H-Index: 1
#2Zhonghui Qiao (Northeast Normal University)
Last. Yifa Zhou (Northeast Normal University)H-Index: 29
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Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum. Their chemical structures investigated by IR, NMR, enzymolysis and methylation analysis indicate that both IPS and EPS are galactomannans composed of α-1,2- mannopyranose (Manp) and α-1,6-Manp in a backbone ratio of ~3:1, respectively, both decorated with β-l,5-galactofuranose (Galf...
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#1Xin Ning (Northeast Normal University)H-Index: 1
#2Ying Liu (Northeast Normal University)
Last. Lin Sun (Northeast Normal University)H-Index: 14
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Abstract Two pectic polysaccharides (WRSP-A2b and WRSP-A3a) have been obtained from Radix Sophorae Tonkinensis and comparatively investigated in terms of their physical properties and antioxidant activities. Monosaccharide composition, FT-IR, NMR and enzymatic analyses indicate that both WRSP-A2b (13.6 kDa) and WRSP-A3a (44.6 kDa) consist of homogalacturonan (HG), rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II) domains, with mass ratios of 0.9:1.8:1 and 2.3:2.9:1, respectively. The...
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#1Zihan Zhao (Northeast Normal University)H-Index: 2
#2Xuejiao Xu (Northeast Normal University)H-Index: 1
Last. Yifa Zhou (Northeast Normal University)H-Index: 29
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Galectin-3 (Gal-3) has a long, aperiodic, and dynamic proline-rich N-terminal tail (NT). The functional role of the NT with its numerous prolines has remained enigmatic since its discovery. To provide some resolution to this puzzle, we individually mutated all 14 NT prolines over the first 68 residues and assessed their effects on various Gal-3-mediated functions. Our findings show that mutation of any single proline (especially P37A, P55A, P60A, P64A/H, and P67A) dramatically and differentially...
1 CitationsSource
#1Yunhe Qu (Northeast Normal University)H-Index: 5
#2Xiaolin Zhao (Northeast Normal University)
Last. Lin Sun (Northeast Normal University)H-Index: 14
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The present study was designed to investigate the structure and immunomodulatory activity of a polysaccharide. A novel acidic β‑glucan (WCCP‑A‑b; molecular weight, 7.3 kDa) was purified from the fruiting bodies of the edible mushroom Cantharellus cibarius, which possesses high nutritional values. WCCP‑A‑b was composed primarily of glucose (89.7%) and glucuronic acid (8.8%). Methylation and nuclear magnetic resonance analysis suggested that WCCP‑A‑b contained β‑D‑1,6‑glucan as its main chain, whi...
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#1Yi Zheng (Northeast Normal University)H-Index: 6
#2Jiyong Su (Northeast Normal University)H-Index: 10
Last. Guihua Tai (Northeast Normal University)H-Index: 21
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Galectin-3 is crucial to many physiological and pathological processes. The generally accepted dogma is that galectins function extracellularly by binding specifically to β(1→4)-galactoside epitopes on cell surface glycoconjugates. Here, we used crystallography and NMR spectroscopy to demonstrate that negatively charged homogalacturonans (HG, linear polysaccharides of α(1→4)-linked-D-galacturonate (GalA)) bind to the galectin-3 carbohydrate recognition domain. The HG carboxylates at the C6 posit...
1 CitationsSource
#1Yanzhe LiuH-Index: 2
#2Dayun Sui (JLU: Jilin University)H-Index: 10
Last. Huali Xu
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Panax ginseng is a traditional medicine used in China to treat many diseases. Polysaccharides are primary active components and have many pharmacological effects. Gastric ulcer is a serious gastrointestinal disease. However, whether polysaccharides influence gastric ulcers is unclear. In this study, the effective gastroprotective impacts and potential mechanisms of Panax ginseng polysaccharides (GPS) on gastric damage induced by ethanol in rats were investigated by macroscopically evaluating gas...
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#1Xinyu Zhang (UJN: University of Jinan)H-Index: 2
#2Guicong Yu (UJN: University of Jinan)
Last. Juan Gao (UJN: University of Jinan)H-Index: 4
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Abstract Lactobacillus plantarum is a useful microorganism that metabolizes galactose-containing polysaccharides. Genome analysis has shown that L. plantarum contains four β-galactosidase-related genes. Here, we cloned the β-galactosidase gene that encodes the glycoside hydrolase family 2 (GH2) enzyme LpGal2. Recombinant LpGal2 (rLpGal2, 72 kDa) is a homodimer with maximal enzymatic activity at pH 7.0 and 50 °C. Under these conditions, rLpGal2 hydrolyzes p-nitrophenyl-β-D-galactopyranoside (pNPβ...
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#1Jing Cui (Northeast Normal University)
#1Jing Cui (Changchun Normal University)H-Index: 2
Last. Yuying Fan (Northeast Normal University)H-Index: 13
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Abstract Ethnopharmacological Relevance Ginsenoside Rg2 is an important ingredient of Panax ginseng which often appears in ancient prescriptions for forgetfulness. Ginsenoside Rg2 exert neuroprotective effects and could be a new potential medicine to treat AD. In our previous study, we reported that ginsenoside Rg2 appears protect PC12 cells against Amyloid β-fragment (25-35) (Aβ25-35)-induced apoptosis via the PI3K/Akt pathway. However, there are no in vivo studies on the protective effects of ...
3 CitationsSource
#1Xiaoxia Peng (Shanxi University)H-Index: 2
#2Guang Yang (Northeast Normal University)H-Index: 6
Last. Mengshan Zhang (Northeast Normal University)H-Index: 4
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A natural low-methoxyl pectin (termed AHP, ) was extracted from dried heads of sunflower and showed better film-forming performance blended with hydroxypropyl methylcellulose (HPMC). The solutions and films of different HPMC/AHP blends were characterized by viscosity, transparency, mechanical properties, loss on drying, water drop penetration time (WDPT), disintegration and SEM. In order to analyze the structure-property relationship of film forming, AHP was separated by ion-exchange chromatogra...
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