Randy M. Whittal
University of California, San Francisco
MALDI imagingTranslocaseAnalytical chemistryBiophysicsPeroxidaseMass spectrometryTime-of-flight mass spectrometrySample preparation in mass spectrometryChemistryProtein foldingPeptideSteroidogenic acute regulatory proteinPregnenoloneMitochondrionElectrospray ionizationChromatographyBiochemistryMatrix-assisted laser desorption/ionizationInner mitochondrial membraneCholesterol side-chain cleavage enzymeBiologyResolution (mass spectrometry)Cell biology
84Publications
33H-index
4,212Citations
Publications 84
Newest
#1Himangshu S. Bose (Mercer University)H-Index: 24
#2Randy M. WhittalH-Index: 33
Last. Neal M Hall (Mercer University)
view all 13 authors...
Estradiol is essential for the development of female sex characteristics and fertility. Postmenopausal women and breast cancer patients have high levels of estradiol. Aromatase catalyzes estradiol synthesis; however, the factors regulating aromatase activity are unknown. We identified a new 22-kDa protein, aromatase interacting partner in breast (AIPB), from the endoplasmic reticulum of human breast tissue. AIPB expression is reduced in tumorigenic breast and further reduced in triple-negative t...
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#1Himangshu S. Bose (Mercer University)H-Index: 24
#2Randy M. Whittal (U of A: University of Alberta)H-Index: 33
Last. Walter L. Miller (UCSF: University of California, San Francisco)H-Index: 110
view all 9 authors...
Aldosterone, which regulates renal salt retention, is synthesized in adrenocortical mitochondria in response to angiotensin II. Excess aldosterone causes myocardial injury and heart failure, but potential intra-cardiac aldosterone synthesis has been controversial. We hypothesized that the stressed heart might produce aldosterone. We used blue native gel electrophoresis, immunoblotting, protein crosslinking, co-immunoprecipitations and mass spectrometry to assess rat cardiac aldosterone synthesis...
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#1Himangshu S. Bose (MUN: Memorial University of Newfoundland)H-Index: 24
#2Brendan MarshallH-Index: 16
Last. Randy M. Whittal (U of A: University of Alberta)H-Index: 33
view all 5 authors...
Summary The first steroidogenic enzyme, cytochrome P450-side-chain-cleavage (SCC), requires electron transport chain (ETC) Complexes III and IV to initiate steroid metabolic processes for mammalian survival. ETC Complex II, containing succinate dehydrogenase (quinone), acts with the TCA cycle and has no proton pumping capacity. We show that Complex II is required for SCC activation through the proton pump, generating an intermediate state for addition of phosphate by succinate. Phosphate anions ...
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#1Jasmine Bhangu (U of A: University of Alberta)H-Index: 2
#2Randy M. Whittal (U of A: University of Alberta)H-Index: 33
Last. Dennis G. Hall (U of A: University of Alberta)H-Index: 80
view all 3 authors...
This study describes the design and synthesis of arylboronic acid 2, the first example of a permanently open 'frustrated' benzoxaborole, along with an exploration of its application in bioconjugation. An efficient and high yielding seven-step synthesis was optimized. NMR experiments confirmed that compound 2 exists in the open ortho-hydroxyalkyl arylboronic acid structure 2-I, a form that is effectively prevented to undergo a dehydrative cyclization as a result of unfavorable geometry. Compound ...
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#1Himangshu S. Bose (Mercer University)H-Index: 24
#2Brendan MarshallH-Index: 16
Last. Randy M. Whittal (Mercer University)H-Index: 33
view all 5 authors...
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#1Himangshu S. Bose (Mercer University)H-Index: 24
#2Randy M. Whittal (Mercer University)H-Index: 33
Last. Walter L. MillerH-Index: 110
view all 8 authors...
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#1Maria Lorna A. De Leoz (NIST: National Institute of Standards and Technology)H-Index: 14
#2David L. Duewer (NIST: National Institute of Standards and Technology)H-Index: 22
Last. Stephen E. SteinH-Index: 49
view all 162 authors...
Glycosylation is a topic of intense current interest in the development of biopharmaceuticals because it is related to drug safety and efficacy. This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material. Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions...
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#1Lindsey Y.K. Suh (U of A: University of Alberta)H-Index: 2
#2Dinesh Babu (U of A: University of Alberta)H-Index: 14
Last. Arno G. Siraki (U of A: University of Alberta)H-Index: 21
view all 9 authors...
Abstract Edaravone is considered to be a potent antioxidant drug known to scavenge free radical species and prevent free radical-induced lipid peroxidation. In this study, we investigated the effect of edaravone on the myeloperoxidase (MPO) activity, an enzyme responsible for the production of an array of neutrophil-derived oxidants that can cause cellular damage. The addition of edaravone to the reaction of MPO and hydrogen peroxide (H2O2) significantly enhanced the reduction of MPO Compound II...
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#1Andrew G.M. Morgan (U of A: University of Alberta)H-Index: 5
#2Dinesh Babu (U of A: University of Alberta)H-Index: 14
Last. Arno G. Siraki (U of A: University of Alberta)H-Index: 21
view all 6 authors...
Abstract Phenol red (PR) is the standard pH indicator in various cell and tissue culture media, as it provides a quick check for the health of the culture. PR has also been used in multiple protocols to detect cellular hydrogen peroxide as well as peroxidase activity from human peroxidase enzymes. The majority of promyelocytic leukemia cell lines (e.g. HL-60 cells) express myeloperoxidase (MPO), which may react with PR, especially as the latter is present in cell culture media at sufficient conc...
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#1Dinesh Babu (U of A: University of Alberta)H-Index: 14
#2Andrew G.M. Morgan (U of A: University of Alberta)H-Index: 5
Last. Arno G. Siraki (U of A: University of Alberta)H-Index: 21
view all 7 authors...
Abstract The formation of isonicotinyl-nicotinamide adenine dinucleotide (INH-NAD+) by the mycobacterial catalase-peroxidase enzyme, KatG, was known to be the major component of the mode of action of isoniazid (INH), an anti-tuberculosis drug. However, there are other enzymes that may catalyze this reaction. We have previously reported that neutrophil myeloperoxidase (MPO) is capable of metabolizing INH through the formation of INH-NAD+ adduct, which could be attributed to being a possible mode ...
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