Richard Thomson-Luque
University Hospital Heidelberg
AntigenVirologyAntibodyTranscriptomeParasitologyIn vitroImmunologyTransmission (medicine)AsymptomaticMalariaPlasmodium falciparumReticulocytePlasmodium vivaxBone marrowImmunogenicityBlood stageTransferrin receptorBinding proteinParasite hostingBiologyImmune system
17Publications
9H-index
200Citations
Publications 18
Newest
#1Richard Thomson-Luque (University Hospital Heidelberg)H-Index: 9
#2Lasse Votborg-Novél (MPG: Max Planck Society)H-Index: 1
Last. Boureima Guindo (University of the Sciences)H-Index: 2
view all 22 authors...
Following Plasmodium falciparum infection, individuals can remain asymptomatic, present with mild fever in uncomplicated malaria cases, or show one or more severe malaria symptoms. Several studies have investigated associations between parasite transcription and clinical severity, but no broad conclusions have yet been drawn. Here, we apply a series of bioinformatic approaches based on P. falciparum's tightly regulated transcriptional pattern during its ~48-hour intraerythrocytic developmental c...
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#1Richard Thomson-Luque (University Hospital Heidelberg)H-Index: 9
#2José M. Bautista (Complutense University of Madrid)H-Index: 37
After a century of constant failure to in vitro culture the most widespread human malaria parasite Plasmodium vivax, recent advances have highlighted the difficulties to provide this parasite with a healthy host cell to invade, develop and multiply under in vitro conditions. The actual level of understanding of the heterogeneous populations of cells, framed under the name ‘reticulocyte’ and, importantly, its adequate in vitro progression from very immature reticulocytes to erythrocytes, is far f...
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#1Carolina M. Andrade (University Hospital Heidelberg)H-Index: 3
#2Hannah Fleckenstein (University Hospital Heidelberg)H-Index: 2
Last. Shanping Li (NIH: National Institutes of Health)H-Index: 19
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The dry season is a major challenge for Plasmodium falciparum parasites in many malaria endemic regions, where water availability limits mosquito vectors to only part of the year. How P. falciparum bridges two transmission seasons months apart, without being cleared by the human host or compromising host survival, is poorly understood. Here we show that low levels of P. falciparum parasites persist in the blood of asymptomatic Malian individuals during the 5- to 6-month dry season, rarely causin...
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Last. Silvia PortugalH-Index: 22
view all 37 authors...
#1Richard Thomson-Luque (University Hospital Heidelberg)H-Index: 9
#2John Adams (USF: University of South Florida)H-Index: 72
Last. Erica M. Pasini (Biomedical Primate Research Centre)H-Index: 17
view all 4 authors...
Historically neglected, due to its biological peculiarities, the absence of a continuous long-term in vitro blood stage culture system and a propensity towards high morbidity rather than mortality, Plasmodium vivax was put back on the agenda during the last decade by the paradigm shift in the fight against malaria from malaria control to malaria eradication. While the incidence of the deadliest form of malaria, Plasmodium falciparum malaria, has declined since this paradigm shift took hold, the ...
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#1Swamy R. Adapa (USF: University of South Florida)H-Index: 9
#2Rachel A. Taylor (USF: University of South Florida)H-Index: 9
Last. Rays H. Y. Jiang (USF: University of South Florida)H-Index: 28
view all 6 authors...
The lack of a continuous long-term in vitro culture system for Plasmodium vivax severely limits our knowledge of pathophysiology of the most widespread malaria parasite. To gain direct understanding of P. vivax human infections, we used Next Generation Sequencing data mining to unravel parasite in vivo expression profiles for P. vivax, and P. falciparum as comparison. We performed cloud and local computing to extract parasite transcriptomes from publicly available raw data of human blood samples...
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#1Richard Thomson-Luque (USF: University of South Florida)H-Index: 9
#2Chengqi Wang (USF: University of South Florida)H-Index: 10
Last. Rays H. Y. Jiang (USF: University of South Florida)H-Index: 28
view all 10 authors...
Abstract Progress towards an in-depth understanding of the final steps of the erythroid lineage development is paramount for many hematological diseases. We have characterized the final stages of reticulocyte maturation from bone marrow to peripheral blood using for the first time single-cell Mass Cytometry (CyTOF). We were able to measure the expression of 31 surface markers within a single red blood cell (RBC). We demonstrate the validity of CyTOF for RBC phenotyping by confirming the progress...
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#1Francis B. Ntumngia (USF: University of South Florida)H-Index: 14
#2Richard Thomson-Luque (USF: University of South Florida)H-Index: 9
Last. John Adams (USF: University of South Florida)H-Index: 72
view all 10 authors...
Erythrocyte invasion by malaria parasites is essential for blood-stage development. Consequently, parasite proteins critically involved in erythrocyte invasion, such as the Plasmodium vivax reticulocyte binding proteins (RBPs) that mediate preferential invasion of reticulocytes, are considered potential vaccine targets. Thus, targeting the RBPs could prevent blood-stage infection and disease. The RBPs are large, and little is known about their functional domains and whether individuals naturally...
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#1Alison Roth (USF: University of South Florida)H-Index: 7
#2Steven P. Maher (USF: University of South Florida)H-Index: 12
Last. John Adams (USF: University of South Florida)H-Index: 72
view all 27 authors...
The original version of this Article contained an error in the spelling of Richard Thomson-Luque, which was incorrectly given as Richard Thomson Luque. This error has now been corrected in both the PDF and HTML versions of the Article.
Source
#1Alison Roth (USF: University of South Florida)H-Index: 7
#2Steven P. Maher (USF: University of South Florida)H-Index: 12
Last. John Adams (USF: University of South Florida)H-Index: 72
view all 27 authors...
Malaria liver stages represent an ideal therapeutic target with a bottleneck in parasite load and reduced clinical symptoms; however, current in vitro pre-erythrocytic (PE) models for Plasmodium vivax and P. falciparum lack the efficiency necessary for rapid identification and effective evaluation of new vaccines and drugs, especially targeting late liver-stage development and hypnozoites. Herein we report the development of a 384-well plate culture system using commercially available materials,...
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