Camilla Siciliano
Sapienza University of Rome
Cell therapyClonogenic assayPhenotypePathologyReceptorAdipose tissueMesenchymal stem cellProgenitor cellIn vitroImmunologyPlatelet lysateStem-cell therapyStem cellTransdifferentiationCell growthMedicineParacrine signallingPI3K/AKT/mTOR pathwayBiologyCell biology
17Publications
10H-index
204Citations
Publications 17
Newest
#1Rita Businaro (Sapienza University of Rome)H-Index: 6
#1Rita Businaro (Sapienza University of Rome)H-Index: 18
Last. Elena De Falco (Sapienza University of Rome)H-Index: 19
view all 14 authors...
Neuropeptide Y (NPY), a powerful neurotransmitter of the central nervous system, is a key regulator of angiogenesis and biology of adipose depots. Intriguingly, its peripheral vascular and angiogenic powerful activity is strictly associated to platelets, which are source of clinical hemoderivates, such as platelet lysate (PL), routinely employed in several clinical applications as wound healing, and to preserve ex vivo the progenitor properties of the adipose stromal cells pool. So far, the pres...
8 CitationsSource
#1Francesca Pagano (Sapienza University of Rome)H-Index: 13
#2Francesco Angelini (Sapienza University of Rome)H-Index: 14
Last. Isotta Chimenti (Sapienza University of Rome)H-Index: 22
view all 10 authors...
Abstract Human cardiac progenitor cells (CPCs) offer great promises to cardiac cell therapy for heart failure. Many in vivo studies have shown their therapeutic benefits, paving the way for clinical translation. The 3D model of cardiospheres (CSs) represents a unique niche-like in vitro microenvironment, which includes CPCs and supporting cells. CSs have been shown to form through a process mediated by epithelial-to-mesenchymal transition (EMT). β2-Adrenergic signaling significantly affects stem...
14 CitationsSource
#1Francesca Pagano (Sapienza University of Rome)H-Index: 13
#2Cristina Nocella (Sapienza University of Rome)H-Index: 26
Last. Giacomo Frati (Sapienza University of Rome)H-Index: 47
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: Respiratory diseases represent a major healthcare burden worldwide. Lung transplantation (LTx) is the "gold standard" for end-stage patients, strongly limited by shortage of available/suitable donor lungs. Normothermic ex vivo lung perfusion (EVLP) has significantly increased the number of lungs suitable for transplantation. Steen solution is used for EVLP, but the mechanisms involved in its beneficial properties remain to be clarified. We investigated the effects of Steen solution in an in vi...
7 CitationsSource
#1Isotta Chimenti (Sapienza University of Rome)H-Index: 22
#2Francesca Pagano (Sapienza University of Rome)H-Index: 13
Last. Giacomo Frati (Sapienza University of Rome)H-Index: 47
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: Basic and translational research on lung biology has discovered multiple progenitor cell types, specialized or facultative, responsible for turnover, renewal, and repair. Isolation of populations of resident lung progenitor cells (LPCs) has been described by multiple protocols, and some have been successfully applied to healthy human lung tissue. We aimed at understanding how different cell culture conditions may affect, in vitro, the phenotype of LPCs to create an ideal niche-like microenviro...
18 CitationsSource
#1Elena De Falco (Sapienza University of Rome)H-Index: 19
#2Roberto Carnevale (Sapienza University of Rome)H-Index: 36
Last. Giacomo Frati (Sapienza University of Rome)H-Index: 47
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Abstract Senescence exerts a great impact on both biological and functional properties of circulating endothelial progenitor cells (EPCs), especially in cardiovascular diseases where the physiological process of aging is accelerated upon clinical administration of certain drugs such as doxorubicin. EPC impairment contributes to doxorubicin-induced cardiotoxicity. Doxorubicin accelerates EPC aging, although mechanisms underlying this phenomenon remain to be fully clarified. Here we investigated i...
24 CitationsSource
#1Camilla Siciliano (Sapienza University of Rome)H-Index: 10
#2Antonella Bordin (Sapienza University of Rome)H-Index: 8
Last. Elena De Falco (Sapienza University of Rome)H-Index: 19
view all 14 authors...
Abstract Indirect evidence suggests that adipose tissue-derived stromal cells (ASCs) possess different physiological and biological variations related to the anatomical localization of the adipose depots. Accordingly, to investigate the influence of the tissue origin on the intrinsic properties of ASCs and to assess their response to specific stimuli, we compared the biological, functional and ultrastructural properties of two ASC pools derived from mediastinal and subcutaneous depots (thoracic ...
16 CitationsSource
#1Rita Businaro (Sapienza University of Rome)H-Index: 18
#2Mariangela Corsi (Sapienza University of Rome)H-Index: 7
Last. Elena De Falco (Sapienza University of Rome)H-Index: 19
view all 11 authors...
New civil wars and waves of terrorism are causing crucial social changes, with consequences in all fields, including health care. In particular, skin injuries are evolving as an epidemic issue. From a physiological standpoint, although wound repair takes place more rapidly in the skin than in other tissues, it is still a complex organ to reconstruct. Genetic and clinical variables, such as diabetes, smoking, and inflammatory/immunological pathologies, are also important risk factors limiting the...
8 CitationsSource
#1Augusto Carducci (Sapienza University of Rome)H-Index: 2
#2Gaia Scafetta (Sapienza University of Rome)H-Index: 7
Last. Elena De Falco (Sapienza University of Rome)H-Index: 19
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Tenon's fibroblasts (TFs), widely employed as in vitro model for many ophthalmological studies, are routinely cultured with FBS. Platelet Lysate (PL), a hemoderivate enriched with growth factors and cytokines has been largely tested in several clinical applications and as substitute of FBS in culture. Here, we investigate whether PL can exert biological effects on TF populations similarly to other cell types. Results show that PL significantly enhances cell proliferation and migration vs. FBS, w...
8 CitationsSource
#1Camilla Siciliano (IIT: Istituto Italiano di Tecnologia)H-Index: 10
#2Isotta ChimentiH-Index: 22
Last. Elena De FalcoH-Index: 19
view all 11 authors...
Nowadays, cardiac regenerative medicine is facing many limitations because of the complexity to find the most suitable stem cell source and to understand the regenerative mechanisms involved. Mesenchymal stem cells (MSCs) have shown great regenerative potential due to their intrinsic properties and ability to restore cardiac functionality, directly by transdifferentiation and indirectly by paracrine effects. Yet, how MSCs could respond to definite cardiac-committing microenvironments, such as th...
19 CitationsSource
#1Camilla Siciliano (IIT: Istituto Italiano di Tecnologia)H-Index: 10
#2Isotta ChimentiH-Index: 22
Last. Elena De FalcoH-Index: 19
view all 11 authors...
Human adipose tissue-derived mesenchymal stem cells (ADMSCs) are considered eligible candidates for cardiovascular stem cell therapy applications due to their cardiac transdifferentiation potential and immunotolerance. Over the years, the in vitro culture of ADMSCs by platelet lysate (PL), a hemoderivate containing numerous growth factors and cytokines derived from platelet pools, has allowed achieving a safe and reproducible methodology to obtain high cell yield prior to clinical administration...
10 CitationsSource