Jian-Ming Gao
University of Agriculture, Faisalabad
EndocrinologyEmbryonic stem cellMolecular biologyAndrologyChemistryApoptosisEmbryoLipid dropletOocytePTENBlastocystRHEBBerberineIcariinCell growthBlotPI3K/AKT/mTOR pathwayBiologyEmbryogenesisEukaryotic initiation factor
8Publications
3H-index
43Citations
Publications 7
Newest
#1Bing Liu (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 2
#2Jun-Li Wang (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 2
Last. Jian-Ming Gao (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 3
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Abstract To explore the repair effect of lycium barbarum polysaccharide (LBP) on ovarian injuries induced by repeated superovulation in mice, a model of ovarian injury was established, and ovarian repair was assessed after intragastric administration of LBP. The oocyte quality and blastocyst rates of pronuclear embryos in vitro were observed. The levels of 8-hydroxydeoxyguanosine (8-OHdG) and lipid peroxide (LPO) in ovarian tissue were measured, and ovarian damage was assessed in paraffin sectio...
1 CitationsSource
#1Jun-Li Wang (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 2
#2Chao Zhang (CAU: China Agricultural University)H-Index: 94
Last. Jian-Ming Gao (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 3
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: The effect of berberine (Ber) on in vitro fertilization (IVF) embryo development in pigs and the associated differential expression of microRNAs (miRNAs) in the embryo were investigated. NCSU-23 embryonic culture medium was used for a control group, while NCSU-23 embryonic culture medium added with Ber was used for a Ber group. The embryo development rates in these groups were determined, and the zygotes, 4- and 8-cell embryos, and blastocysts were collected for cDNA microarray analysis. The d...
4 CitationsSource
#1Jun-Li Wang (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 2
#2Bing Liu (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 2
Last. Jian-Ming Gao (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 3
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Abstract In this study, effects of icariin (Ica) on were examined in a mouse model of d -galactose (D-gal)-induced ovarian aging. Kunming white mice were divided into three groups: aging group induced with D-gal, experiment group treated with Ica at low (50 mg/kg), middle (100 mg/kg) and high (200 mg/kg) concentrations, and control group with no treatment. Ovarian histomorphology, serum FSH, LH and E2 levels, and reproductive function were compared among the groups. Ovarian expression of Amh, Ba...
13 CitationsSource
#1Chao Zhang (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 3
#1Chao Zhang (PKU: Peking University)H-Index: 94
Last. Jian-Ming Gao (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 3
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The aim of the present study was to clarify the roles of the mammalian target of rapamycin (mTOR) signalling pathway in follicular growth and development of thecal cells. Using in vivo-grown and in vitro-cultured ovaries, histological changes were evaluated using haematoxylin and eosin (HE) staining. Differentially expressed genes (DEGs) from 0 day post partum (d.p.p.) to 8 d.p.p. ovaries were screened by microarray and verified by quantitative real-time polymerase chain reaction. Forty-two DEGs...
2 CitationsSource
#1Chao Zhang (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 3
#1Chao Zhang (PKU: Peking University)H-Index: 94
Last. Jian-Ming Gao (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 3
view all 9 authors...
Traditional Chinese medicinal herbs containing berberine have been historically used to prevent miscarriage. Here, we investigated whether the anti-apoptotic effects of berberine on pre-implantation embryonic development are regulated by miRNA-21. Mouse pronuclear embryos were cultured in medium with or without berberine, and some were then microinjected with a miRNA-21 inhibitor. The in vitro developmental rates of 2- and 4-cell embryos and blastocysts, blastocyst cell numbers, apoptotic rates,...
11 CitationsSource
#1Chao Zhang (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 3
#1Chao Zhang (PKU: Peking University)H-Index: 94
Last. Jian-Ming Gao (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 3
view all 9 authors...
Abstract We constructed a model of apoptosis in mouse preimplantation embryos and investigated the effect of the flavonol icariin on embryonic development in vitro in embryos with reduced microRNA-21 (miR-21). The model was generated by microinjecting an miR-21 inhibitor into the cytoplasm of mouse pronuclear embryos, which were cultured in vitro using modified CZB (mCZB) basal medium (model group), or using mCZB medium with 0.6 μg/mL icariin as an experimental group (model-Ica). These were comp...
7 CitationsSource
#1Ya-Ran Shi (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 3
#2Zhan-he Wang (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 1
Last. Jian-Ming Gao (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 3
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Abstract In this study, the effect of icariin, a flavonoid from the Chinese traditional medicine epimedium, on miRNA-21 of mouse developmental blastocysts in vitro and the development of preimplantation embryos were studied. The possible effective targets of icariin promoting preimplantation embryo development in vitro and anti-apoptosis were determined. The embryos were cultured in modified CZB medium (mCZB) as control group. The experimental group (Ica group) was supplemented with 0.6 μg mL−1 ...
3 CitationsSource