Wolfgang Kaiser
Technische Universität München
Analytical chemistryOpticsPhysicsSemiconductor laser theoryFemtosecondUltrashort pulseExcited stateAtomic physicsChemistryMaterials scienceQuantum wellMoleculeRaman spectroscopyOptoelectronicsLaserPicosecondAbsorption (electromagnetic radiation)Quantum dot laserPhotochemistryExcitation
159Publications
30H-index
5,371Citations
Publications 111
Newest
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#1Felix Kroener (TUD: Dresden University of Technology)H-Index: 2
#2Andreas HeerwigH-Index: 12
Last. Ulrich RantH-Index: 26
view all 5 authors...
Development of electrically powered DNA origami nanomachines requires effective means to actuate moving origami parts by externally applied electric fields. We demonstrate how origami nanolevers on an electrode can be manipulated (switched) at high frequency by alternating voltages. Orientation switching is long-time stable and can be induced by applying low voltages of 200 mV. The mechanical response time of a 100 nm long origami lever to an applied voltage step is less than 100 μs, allowing dy...
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#1Thomas Welte (TUM: Technische Universität München)H-Index: 5
#2Ralf StrasserH-Index: 6
Last. Ulrich RantH-Index: 26
view all 5 authors...
switchSENSE is a novel chip-based bioanalytical method that employs electrically switchable DNA nanolevers as molecular probes. While the nanolevers are driven to oscillate on the surface of microelectrodes by alternating electric fields, their orientation-switching is detected by time-resolved single photon counting. By measuring the molecular dynamics of the switching process it is possible to gauge the hydrodynamic properties of analyte molecules which are bound to the oscillating nanolevers....
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#1Andreas Langer (TUM: Technische Universität München)H-Index: 8
#2Wolfgang KaiserH-Index: 30
Last. Ulrich Rant (TUM: Technische Universität München)H-Index: 26
view all 5 authors...
Self-assembled monolayers of charged polymers are an integral component of many state-of-the-art nanobiosensors. Electrical interactions between charged surfaces and charged biomolecules, adopting the roles of linkers or capture molecules, are not only crucial to the sensor performance but may also be exploited for novel sensing concepts based on electrically actuated interfaces. Here we introduce an analytical model describing the behavior of double-stranded DNA and proteins tethered to externa...
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#1Andreas Langer (TUM: Technische Universität München)H-Index: 8
#2Paul A. Hampel (TUM: Technische Universität München)H-Index: 3
Last. Ulrich Rant (TUM: Technische Universität München)H-Index: 26
view all 12 authors...
The comprehensive bioanalysis of proteins usually requires multi-step surface and mobile phase measurements. Here, the authors use chips functionalized with dynamically actuated nanolevers—DNA strands that can be switched in an electric field—to obtain motional dynamic measurements of proteins on a chip.
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#1Andreas LangerH-Index: 8
#2Paul A. HampelH-Index: 3
Last. Ulrich RantH-Index: 26
view all 10 authors...
Powerful methods for analyzing proteins and their interactions with small molecules, nucleic acids or other proteins are a cornerstone in the development of new drugs and next-generation disease diagnostics. Commonly, affinity and kinetics of molecular interactions are determined with surface biosensors, which measure the adsorption/desorption of solute target molecules to/from surface immobilized capture molecules. However, information on protein size, shape (folding state), molecular aggregate...
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#1Jelena Knezevic (TUM: Technische Universität München)H-Index: 4
#2Andreas LangerH-Index: 8
Last. Ulrich RantH-Index: 26
view all 6 authors...
A label-free method for the analysis of interactions of proteins with surface-tethered ligands is introduced. Short DNA levers are electrically actuated on microelectrodes by ac potentials, and their switching dynamics are measured in real-time by fluorescence energy transfer. Binding of proteins to ligands attached to the top of the DNA levers is detected by time-resolved measurements of the levers’ dynamic motion. We demonstrate the quantitation of binding kinetics (kon, koff rate constants), ...
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#1Donald F. NelsonH-Index: 2
#2Robert J. CollinsH-Index: 1
Last. Wolfgang KaiserH-Index: 30
view all 3 authors...
#1Wolfgang Kaiser (TUM: Technische Universität München)H-Index: 30
#2Ulrich RantH-Index: 26
We describe the behavior of 72mer oligonucleotides that are end-tethered to gold surfaces under the influence of applied electric fields. The DNA extension is measured by fluorescence energy transfer as a function of the DNA hybridization state (single- and double-stranded), the concentration of monovalent salt in solution (100 μM to 1 M NaCl), the applied electrode potential (−0.6 to +0.1 V vs Pt), and the temperature (1 to 50 °C). At high ionic strength, the DNA conformations are very robust a...
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#1Donald F. NelsonH-Index: 2
#2Robert J. CollinsH-Index: 1
Last. Wolfgang KaiserH-Index: 30
view all 3 authors...
In 1960 two rival laboratories reported the creation of lasers. Controversy over priority and proper behavior has persisted for half a century. Now three Bell Labs veterans of that confused but exciting summer tell the story as they remember it.
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