Bhavani S. Shankar
Bhabha Atomic Research Centre
CytotoxicityDNA damageMolecular biologyCytotoxic T cellChemistryApoptosisConcanavalin AImmunologyPropidium iodideGrowth factorT cellTumor microenvironmentOxidative stressAntioxidantDendritic cellSpleenProgrammed cell deathCancer researchNitric oxideFlow cytometryMAPK/ERK pathwayIntracellularCell cultureBiologyImmune systemCell biology
34Publications
12H-index
460Citations
Publications 29
Newest
#1Kavitha Premkumar (BARC: Bhabha Atomic Research Centre)H-Index: 2
#2Prayag J. Amin (BARC: Bhabha Atomic Research Centre)H-Index: 5
Last. Bhavani S. Shankar (BARC: Bhabha Atomic Research Centre)H-Index: 12
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Abstract Tinospora cordifolia is a medically valuable plant widely used in Ayurveda and has been attributed with immunomodulatory properties. We compared the immunomodulation of repeated doses of the purified polysaccharide G1-4A and polysaccharide-rich extract (PRE) of T. cordifolia in BALB/c mice. Since both had comparable effects, we further compared the repeated dose effects of 50 and 500 mg/kg b.w PRE per dose with particular emphasis on histological changes in different organs, splenic cel...
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#1Poonam Yadav (HBNI: Homi Bhabha National Institute)H-Index: 1
#2Vipul K. Pandey (BARC: Bhabha Atomic Research Centre)H-Index: 4
Last. Bhavani S. Shankar (HBNI: Homi Bhabha National Institute)H-Index: 12
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Our previous studies have shown that MCF-7 breast cancer cell line exposed to 6 Gy and allowed to recover for 7 days (D7-6G) developed radio-resistance. In this study, we have tested the ability of these cells to form tumors in severe combined immunodeficiency (SCID) mice and characterized these tumors by proteomic analyses. Untreated (MCF-C) and D7-6G cells (MCF-R) were injected s.c. in SCID mice and tumor growth monitored. On Day 18, the mice were killed and tumor tissues were fixed in formali...
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#1Archana Mishra (BARC: Bhabha Atomic Research Centre)H-Index: 6
Biohybrids (a combination of biological material and inorganic nanoparticles) offer a number of advantages like improved functionality over conventional materials.Thus, to understand the practical application of biohybrids as drug carriers, a biohybrid drug carrier of colloidal silica nanoparticles (NP)-sodium alginate loaded with doxorubicin (Dox-biohybrid) was synthesized by evaporation induced self-assembly (EISA) using spray drying technique. Further, the morphology, size and interactions be...
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#1Kavitha Premkumar (HBNI: Homi Bhabha National Institute)H-Index: 2
#2Bhavani S. Shankar (HBNI: Homi Bhabha National Institute)H-Index: 12
The contribution of immune cells in soft tissue sarcomas (STS) is not completely known and understanding their role is very essential for employing immunotherapy strategies. Here, we show that murine fibrosarcoma-conditioned medium promoted total spleen cell proliferation but inhibited T cell responses to mitogenic and allo-antigen-mediated stimulation. This increased proliferation was found to be in B cells resulting in generation of Breg further leading to Treg population. This was found to be...
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#1Vipul K. Pandey (BARC: Bhabha Atomic Research Centre)H-Index: 4
#2Bhavani S. Shankar (HBNI: Homi Bhabha National Institute)H-Index: 12
: Purpose: To evaluate the effect of ionizing radiation exposure on differentiation and maturation of dendritic cells (DC).Materials and Methods: Bone marrow progenitor cells irradiated in vitro or isolated from mice exposed to whole body or localised tumour irradiation were differentiated into DC. Phenotypic maturation of DC was characterised by labelling with specific antibodies and flow cytometry analysis. Cytokines were estimated by ELISA.Results: Splenic and bone marrow derived DC from tumo...
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#1Poonam Yadav (HBNI: Homi Bhabha National Institute)H-Index: 1
#2Bhavani S. Shankar (HBNI: Homi Bhabha National Institute)H-Index: 12
Abstract Aims A major obstacle for effective cancer treatment by radiation therapy is the development of radio-resistance and identification of underlying mechanisms and activated pathways will lead to better combination therapies. Main methods Irradiated MCF-7 and MDA-MB-231 breast cancer cell lines were characterised following different recovery periods. Proliferation was assessed by MTT, BrdU and clonogenic assays and apoptosis by Annexin V/ propidium iodide staining and flow cytometry. Gene ...
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#1Kavitha Premkumar (BARC: Bhabha Atomic Research Centre)H-Index: 2
#2Jisha Nair (BARC: Bhabha Atomic Research Centre)H-Index: 1
Last. Bhavani S. Shankar (BARC: Bhabha Atomic Research Centre)H-Index: 12
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AbstractPurpose: Our earlier studies demonstrated that transient radio-adaptive responses (RAR) in BALB/c mice were due to MAPK hyperactivation. The objective of this study was to determine the time duration of this low dose induced MAPK activation in BALB/c mice and to find out if similar adaptive responses are observed in C57BL/6 mice.Materials and methods: Mice were irradiated with 0.1 Gy priming dose (PD), 2 Gy challenge dose (CD) with an interval of 4 h (P + CD) and radiation induced immuno...
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#1Rajshri Singh (BARC: Bhabha Atomic Research Centre)H-Index: 2
#2Priya Dagar (BARC: Bhabha Atomic Research Centre)H-Index: 1
Last. Bhavani S. Shankar (BARC: Bhabha Atomic Research Centre)H-Index: 12
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Abstract Background Tumor microenvironment is composed of a largely altered extracellular matrix with different cell types. The complex interplay between macrophages and tumor cells through several soluble factors and signaling is an important factor in breast cancer progression. Methods We have extended our earlier studies on monocyte and macrophage conditioned medium (MϕCM) and have carried out proteomic analysis to identify its constituents as well as validation. The 8-gene signature identifi...
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#1Shyama Pal (BARC: Bhabha Atomic Research Centre)H-Index: 4
#2Poonam Yadav (BARC: Bhabha Atomic Research Centre)H-Index: 1
Last. Bhavani S. Shankar (BARC: Bhabha Atomic Research Centre)H-Index: 12
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Abstract The mechanism by which tumor microenvironment derived cytokine network modulates therapy response is of great concern in lung cancer but is not completely understood. In this study, we evaluated the effects of tumor necrosis factor α (TNF-α) and insulin-like growth factor 1 (IGF-1) on response of lung cancer cell lines to ionizing radiation (IR). While TNF-α increased radio sensitivity and inhibited cell migration, treatment with IGF-1 promoted cell growth and increased migration. These...
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#1Priya DagarH-Index: 1
#2Bhavani S. Shankar (BARC: Bhabha Atomic Research Centre)H-Index: 12
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