Atsuo Ogura
University of Tsukuba
GeneSpermatogenesisInternal medicineEndocrinologySomatic cellEmbryonic stem cellCellular differentiationMolecular biologyAndrologyEmbryoGenomic imprintingSomatic cell nuclear transferCloningImmunologySpermStem cellTransplantationGeneticsBiologyCell biology
Publications 326
#1Hiroko Morimoto (Kyoto University)H-Index: 19
#2Narumi OgonukiH-Index: 53
Last. Takashi Shinohara (Kyoto University)H-Index: 57
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Spermatogonial transplantation has been used as a standard assay for spermatogonial stem cells (SSCs). After transplantation into the seminiferous tubules, SSCs transmigrate through the blood-testis barrier (BTB) between Sertoli cells and settle in a niche. Unlike in the repair of other self-renewing systems, SSC transplantation is generally performed after complete destruction of endogenous spermatogenesis. Here, we examined the impacts of recipient conditioning on SSC homing. Germ cell ablatio...
#1Satoshi KamimuraH-Index: 13
#2Kimiko Inoue (University of Tsukuba)H-Index: 53
Last. Atsuo Ogura (University of Tsukuba)H-Index: 75
view all 15 authors...
In mammalian cloning by somatic cell nuclear transfer (SCNT), treatment of reconstructed embryos with histone deacetylase (HDAC) inhibitors improves efficiency. So far, most of those used for SCNT are hydroxamic acid derivatives-such as trichostatin A-characterized by their broad inhibitory spectrum. Here, we examined whether mouse SCNT efficiency could be improved using chlamydocin analogues, a family of newly designed agents that specifically inhibit Class I and IIa HDACs. Development of SCNT-...
#1Kento Miura (Hiroshima University)H-Index: 6
#2Atsuo Ogura (University of Tsukuba)H-Index: 75
Last. Osamu Kaminuma (Hiroshima University)H-Index: 23
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Following the development of genome editing technology, it has become more feasible to create genetically modified animals such as knockout (KO), knock-in, and point-mutated animals. The genome-edited animals are useful to investigate the roles of various functional genes in many fields of biological science including radiation research. Nevertheless, some researchers may experience difficulty in generating genome-edited animals, probably due to the requirement for equipment and techniques for e...
#1Yuki Kobayashi (YCU: Yokohama City University)H-Index: 1
#2Shin-ichi Tomizawa (YCU: Yokohama City University)H-Index: 10
Last. Kazuyuki Ohbo (YCU: Yokohama City University)H-Index: 14
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During spermatogenesis, intricate gene expression is coordinately regulated by epigenetic modifiers, which are required for differentiation of spermatogonial stem cells (SSCs) contained among undifferentiated spermatogonia. We previously found that KMT2B conveys H3K4me3 at bivalent and monovalent promoters in undifferentiated spermatogonia. Because these genes are expressed late in spermatogenesis or during embryogenesis, we expect that many of them are potentially programmed by KMT2B for future...
#1Shuai Sun (UTokyo: University of Tokyo)
#2Shota Yano (UTokyo: University of Tokyo)
Last. Satoshi Tanaka (UTokyo: University of Tokyo)H-Index: 33
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Mouse trophoblast stem cells (TSCs) can differentiate into trophoblast cells, which constitute the placenta. Under conventional culture conditions, in a medium supplemented with 20% fetal bovine serum (FBS), fibroblast growth factor 4 (FGF4), and heparin and in the presence of mouse embryonic fibroblast cells (MEFs) as feeder cells, TSCs maintain their undifferentiated, proliferative status. MEFs can be replaced by a 70% MEF-conditioned medium (MEF-CM) or by TGF-s/activin A. To find out if Knock...
#1Yoshifumi Mori (Kyoto University)H-Index: 3
#2Narumi OgonukiH-Index: 53
Last. Takashi Shinohara (Kyoto University)H-Index: 57
view all 8 authors...
Although reactive oxygen species (ROS) are required for spermatogonial stem cell (SSC) self-renewal, they induce DNA damage and are harmful to SSCs. However, little is known about how SSCs protect their genome during self-renewal. Here, we report that Ogg1 is essential for SSC protection against ROS. While cultured SSCs exhibited homologous recombination-based DNA double-strand break repair at levels comparable with those in pluripotent stem cells, they were significantly more resistant to hydro...
#1Atsuo OguraH-Index: 75
#2Shogo MatobaH-Index: 26
Last. Kimiko InoueH-Index: 53
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Twenty-five years have passed since the birth of Dolly the sheep, the first mammalian clone produced by adult somatic cell nuclear transfer (SCNT). During that time, the main thrust of SCNT-related research has been the elucidation of SCNT-associated epigenetic abnormalities and their correction, with the aim of improving the efficiency of cloned animal production. Through these studies, it has become clear that some epigenomic information can be reprogrammed by the oocyte, while some cannot. No...
#1Zuzana LoubalovaH-Index: 1
#2Helena FulkaH-Index: 17
Last. Petr SvobodaH-Index: 37
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PIWI-associated RNAs (piRNAs) support the germline by suppressing retrotransposons and genes. In mice, piRNAs are essential for spermatogenesis but not oogenesis. To test how this applies to other mammals, we deleted Mov10l1 helicase in golden hamster, whose piRNA pathway is configured more similarly to that of other mammals. Mov10l1-/- male hamsters showed impaired establishment of spermatogonia accompanied by transcriptome dysregulation and a surge in MYSERV retrotransposon expression. The rar...
1 CitationsSource
#1Hiroko MorimotoH-Index: 19
#2Takuya YamamotoH-Index: 45
Last. Takashi ShinoharaH-Index: 57
view all 12 authors...
Reactive oxygen species (ROS) produced by NADPH1 oxidase 1 (NOX1) are thought to drive spermatogonial stem cell (SSC) self-renewal through feed-forward production of ROS by the ROS-BCL6B-NOX1 pathway. Here we report the critical role of oxygen on ROS-induced self-renewal. Cultured SSCs proliferated poorly and lacked BCL6B expression under hypoxia despite increase in mitochondria-derived ROS. Due to lack of ROS amplification under hypoxia, NOX1-derived ROS were significantly reduced, and Nox1-def...
#1Kento Miura (Hiroshima University)H-Index: 6
#2Shogo Matoba (TUAT: Tokyo University of Agriculture and Technology)H-Index: 26
Last. Atsuo Ogura (University of Tsukuba)H-Index: 75
view all 4 authors...
Conditional knockout (cKO) mice have contributed greatly to understanding the tissue- or stage-specific functions of genes in vivo. However, the current cKO method requires considerable time and effort because of the need to generate two gene-modified mouse strains (Cre transgenic and loxP knockin) for crossing. Here we examined whether we could analyze the germ cell-related functions of embryonic lethal genes in F0 chimeric mice by restricting the origin of germ cells to mutant embryonic stem c...
2 CitationsSource