Zhensheng Li
University of Washington
BiophysicsBiomedical engineeringElectrospinningNanotechnologyMaterials scienceMesenchymal stem cellGrowth factorBone tissueChitosanChondrocytePopulationBiological propertyBone regenerationChemical engineeringTissue engineeringPolymerCell growthCell cultureScaffoldNanofiberCell biology
11Publications
9H-index
1,516Citations
Publications 11
Newest
#1Stephen J. Florczyk (UW: University of Washington)H-Index: 18
#2Matthew Leung (UW: University of Washington)H-Index: 17
Last. Miqin Zhang (UW: University of Washington)H-Index: 81
view all 6 authors...
This study investigated the use of three-dimensional porous chitosan–alginate (CA) scaffolds for critical size calvarial defect (diameter, 5.0 mm) repair in Sprague–Dawley rats. CA scaffolds have been used for in vitro culture of many cell types and demonstrated osteogenesis in ectopic locations in vivo, but have yet to be evaluated for functional bone tissue engineering applications. CA scaffolds demonstrated the ability to support undifferentiated mesenchymal stem cells (MSCs) in culture for 1...
50 CitationsSource
#1Stephen J. Florczyk (UW: University of Washington)H-Index: 18
#2Matthew Leung (UW: University of Washington)H-Index: 17
Last. Miqin Zhang (UW: University of Washington)H-Index: 81
view all 8 authors...
Increasing cell seeding efficiency in a tissue engineering construct can enhance cellular activity and tissue formation in vivo. Here, we demonstrate the use of alginate gel as a secondary phase material in 3D porous β-tricalcium phosphate scaffolds to improve cell seeding and provide controlled release of growth factors for bone tissue engineering. Cells were seeded in scaffolds in three ways: conventional seeding (CS), alginate gel-assisted seeding (GS), and alginate GS with bone morphogenetic...
25 CitationsSource
#1Zhensheng Li (UW: University of Washington)H-Index: 9
#2Matthew Leung (UW: University of Washington)H-Index: 17
Last. Miqin Zhang (UW: University of Washington)H-Index: 81
view all 5 authors...
Human embryonic stem cells (hESCs) are routinely cultured on fibroblast feeder layers or in fibroblast-conditioned medium, which requires continued supply of feeder cells and poses the risks of xenogenic contamination and other complications such as feeder-dependent outcome. Here, we demonstrate a strategy that supports sustained self-renewal of hESCs in a three-dimensional porous natural polymer scaffold, comprised of chitosan and alginate, without the support of feeder cells or conditioned med...
162 CitationsSource
#1Narayan Bhattarai (UW: University of Washington)H-Index: 36
#2Zhensheng Li (UW: University of Washington)H-Index: 9
Last. Miqin Zhang (UW: University of Washington)H-Index: 81
view all 11 authors...
142 CitationsSource
#1Zhensheng Li (UW: University of Washington)H-Index: 9
#2Jonathan Gunn (UW: University of Washington)H-Index: 17
Last. Miqin Zhang (UW: University of Washington)H-Index: 81
view all 5 authors...
High cell density and uniformity in a tissue-engineered construct is essential to expedite the formation of a uniform extracellular matrix. In this study, we demonstrated an on-site gelation approach to increase cellular population and uniformity through porous scaffolds using alginate as gelling material. The on-site gelation was triggered during cell seeding and was shown to effectively restrain the cells in the porous scaffold during subsequent cell cultivation. The initial demonstration of t...
35 CitationsSource
#1Narayan Bhattarai (UW: University of Washington)H-Index: 36
#2Zhensheng Li (UW: University of Washington)H-Index: 9
Last. Miqin Zhang (UW: University of Washington)H-Index: 81
view all 4 authors...
311 CitationsSource
#1Zhensheng Li (UW: University of Washington)H-Index: 9
#2Hassna R. Ramay (UW: University of Washington)H-Index: 7
Last. Miqin Zhang (UW: University of Washington)H-Index: 81
view all 4 authors...
This paper reports the development of a biodegradable porous scaffold made from naturally derived chitosan and alginate polymers for bone tissue engineering. The scaffold has a 3-D interconnected porous structure and was fabricated through thermally induced phase separation. The mechanical test showed that the scaffold has compressive strength of 0.46 ± 0.02 MPa — about 4 times that of the pure chitosan scaffold. The cell-material interaction study indicated that osteoblast cells seeded on the c...
Source
#1Zhensheng Li (UW: University of Washington)H-Index: 9
#2Miqin Zhang (UW: University of Washington)H-Index: 81
Tissue compatibility of chitosan–alginate scaffolds was studied in vitro in terms of cell morphology, proliferation, and functionality using HTB-94 cells. The scaffold has an interconnected 3D porous structure, and was fabricated by thermally induced phase separation followed by freeze drying. Cell proliferation on the chitosan–alginate scaffold was found to be faster than on a pure chitosan scaffold. After cell culture for 2 weeks in vitro, the cells on the chitosan scaffold gradually assumed a...
246 CitationsSource
#1Hassna R. RamayH-Index: 7
#2Zhensheng LiH-Index: 9
Last. Miqin ZhangH-Index: 81
view all 4 authors...
10 CitationsSource
#1Zhensheng Li (UW: University of Washington)H-Index: 9
#2Hassna R. Ramay (UW: University of Washington)H-Index: 7
Last. Miqin Zhang (UW: University of Washington)H-Index: 81
view all 5 authors...
A biodegradable scaffold in tissue engineering serves as a temporary skeleton to accommodate and stimulate new tissue growth. Here we report on the development of a biodegradable porous scaffold made from naturally derived chitosan and alginate polymers with significantly improved mechanical and biological properties as compared to its chitosan counterpart. Enhanced mechanical properties were attributable to the formation of a complex structure of chitosan and alginate. Bone-forming osteoblasts ...
943 CitationsSource