Forest M. White
Massachusetts Institute of Technology
Receptor tyrosine kinasePhosphorylationCell signalingMolecular biologyMass spectrometryKinaseChemistryTyrosine phosphorylationEpidermal growth factor receptorComputer scienceProteomicsCancer researchBiochemistryBioinformaticsSignal transductionPhosphoproteomicsComputational biologyBiologyProtein phosphorylationCell biology
Publications 177
#1Lauren E. StopferH-Index: 6
Last. Forest M. WhiteH-Index: 60
view all 3 authors...
#1Walid M. Abdelmoula (Brigham and Women's Hospital)H-Index: 14
#2Begoña Giménez-Cassina López (Brigham and Women's Hospital)H-Index: 13
Last. Nathalie Y. R. Agar (Brigham and Women's Hospital)H-Index: 32
view all 9 authors...
Mass spectrometry imaging (MSI) is an emerging technology that holds potential for improving, biomarker discovery, metabolomics research, pharmaceutical applications and clinical diagnosis. Despite many solutions being developed, the large data size and high dimensional nature of MSI, especially 3D datasets, still pose computational and memory complexities that hinder accurate identification of biologically relevant molecular patterns. Moreover, the subjectivity in the selection of parameters fo...
Many pathologies, including cancer, have been associated with aberrant phosphorylation-mediated signaling networks that drive altered cell proliferation, migration, metabolic regulation, and can le...
#1Lauren E. Stopfer (MIT: Massachusetts Institute of Technology)H-Index: 6
#2Aaron S. Gajadhar (Thermo Fisher Scientific)H-Index: 3
Last. Forest M. White (MIT: Massachusetts Institute of Technology)H-Index: 60
view all 8 authors...
Absolute quantification measurements (copies per cell) of peptide major histocompatibility complex (pMHC) antigens are necessary to inform targeted immunotherapy drug design; however, existing methods for absolute quantification have critical limitations. Here, we present a platform termed SureQuant-IsoMHC, utilizing a series of pMHC isotopologues and internal standard-triggered targeted mass spectrometry to generate an embedded multipoint calibration curve to determine endogenous pMHC concentra...
#1Forest M. WhiteH-Index: 60
#2Lauren E. StopferH-Index: 6
#1Jamie M. KellyH-Index: 2
#2Peter D. IvattH-Index: 5
Last. Noelle E. SelinH-Index: 44
view all 9 authors...
Scientists and regulators commonly use benzo[a]pyrene concentrations to assess cancer risk from complex mixtures of atmospheric polycyclic aromatic hydrocarbons (PAHs). Here, we show that benzo[a]p...
#1Begoña Giménez-Cassina López (Brigham and Women's Hospital)H-Index: 13
#2Ishwar N. Kohale (MIT: Massachusetts Institute of Technology)H-Index: 1
Last. Michael S. Regan (Brigham and Women's Hospital)H-Index: 7
view all 28 authors...
Background null Response to targeted therapy varies between patients for largely unknown reasons. Here, we developed and applied an integrative platform using mass spectrometry imaging (MSI), phosphoproteomics, and multiplexed tissue imaging for mapping drug distribution, target engagement, and adaptive response to gain insights into heterogeneous response to therapy. null Methods null Patient derived xenograft (PDX) lines of glioblastoma were treated with adavosertib, a Wee-1 inhibitor, and tis...
#1Owen K Leddy (Ragon Institute of MGH, MIT and Harvard)
#2Forest M. White (MIT: Massachusetts Institute of Technology)H-Index: 60
Last. Bryan D. Bryson (Ragon Institute of MGH, MIT and Harvard)H-Index: 15
view all 3 authors...
T cells must recognize pathogen-derived peptides bound to major histocompatibility complexes (MHCs) in order to initiate a cell-mediated immune response against an infection, or to support the development of high-affinity antibody responses. Identifying antigens presented on MHCs by infected cells and professional antigen-presenting cells (APCs) during infection may therefore provide a route toward developing new vaccines. Peptides bound to MHCs can be identified at whole-proteome scale using ma...
#1Ishwar N. Kohale (MIT: Massachusetts Institute of Technology)H-Index: 1
#2Danielle M. Burgenske (Mayo Clinic)H-Index: 2
Last. Forest M. White (MIT: Massachusetts Institute of Technology)H-Index: 60
view all 12 authors...
Human tissue samples commonly preserved as formalin-fixed paraffin-embedded (FFPE) tissues after diagnostic or surgical procedures in the clinic represent an invaluable source of clinical specimens for in-depth characterization of signaling networks to assess therapeutic options. Tyrosine phosphorylation (pTyr) plays a fundamental role in cellular processes and is commonly dysregulated in cancer but has not been studied to date in FFPE samples. Additionally, pTyr analysis that may otherwise info...
Effective immunosurveillance of cancer requires the presentation of peptide antigens on major histocompatibility complex Class I (MHC-I). Recent developments in proteomics have improved the identification of peptides that are naturally presented by MHC-I, collectively known as the "immunopeptidome". Current approaches to profile tumor immunopeptidomes have been limited to in vitro investigation, which fails to capture the in vivo repertoire of MHC-I peptides, or bulk tumor lysates, which are obs...
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